Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L0994_RS02590 Genome accession   NZ_CP090847
Coordinates   512480..514165 (-) Length   561 a.a.
NCBI ID   WP_065100288.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain WXL032     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 507480..519165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0994_RS02560 (L0994_02550) rplS 507998..508351 (+) 354 WP_004735514.1 50S ribosomal protein L19 -
  L0994_RS02565 (L0994_02555) yacG 508637..508834 (-) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  L0994_RS02570 (L0994_02560) zapD 508916..509656 (-) 741 WP_010435917.1 cell division protein ZapD -
  L0994_RS02575 (L0994_02565) coaE 509683..510297 (-) 615 WP_010435920.1 dephospho-CoA kinase -
  L0994_RS02580 (L0994_02570) pilD 510300..511169 (-) 870 WP_373929936.1 A24 family peptidase Machinery gene
  L0994_RS02585 (L0994_02575) pilC 511236..512465 (-) 1230 WP_016768204.1 type II secretion system F family protein Machinery gene
  L0994_RS02590 (L0994_02580) pilB 512480..514165 (-) 1686 WP_065100288.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L0994_RS02595 (L0994_02585) pilA 514165..514638 (-) 474 WP_016790756.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  L0994_RS02600 (L0994_02590) nadC 514892..515782 (-) 891 WP_004738163.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L0994_RS02605 (L0994_02595) ampD 515866..516465 (+) 600 WP_016790757.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L0994_RS02610 (L0994_02600) pdhR 516947..517708 (+) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62090.84 Da        Isoelectric Point: 5.4344

>NTDB_id=645213 L0994_RS02590 WP_065100288.1 512480..514165(-) (pilB) [Vibrio cyclitrophicus strain WXL032]
MLTNLPTVLRQAGLLSLTQEQAVAEHVEASGISTPEALLALNFFSSDSLANNIKTIFGLPLVRLANTDYESLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQVELVVANYSELEGAIRKLYGRSISGQYSKRKEITQDELAN
LVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKTADDSPDIYLRHSIPNSQT
IYKANSQGCNECNQGYSGRVGIYEVMPFTDKLKTSLINKPNALAIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=645213 L0994_RS02590 WP_065100288.1 512480..514165(-) (pilB) [Vibrio cyclitrophicus strain WXL032]
ATGTTAACCAACCTCCCAACCGTTCTCCGTCAGGCTGGCTTACTTAGCCTGACTCAGGAACAAGCTGTTGCGGAACATGT
GGAAGCTTCAGGTATATCTACACCTGAAGCTTTACTCGCTCTGAATTTCTTCAGCAGCGATTCCTTAGCAAACAACATTA
AAACCATCTTCGGCTTACCGTTAGTACGATTAGCCAATACTGACTACGAATCCCTGTGTGAACAATTAGGACTTCGTGAA
CTGATTACTAAGTATCGTGCTATTCCAATTTCAGTTTCTAGTTCAACTCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAGGCTGAAGATGACTTCCGCTTTGCGACAGGATTGCAAGTTGAATTGGTTGTCGCTAACTACTCTGAATTAGAGGGTG
CAATACGAAAATTGTATGGTCGTTCTATTTCCGGGCAATACTCCAAACGCAAAGAGATCACCCAAGATGAGCTAGCTAAC
CTCGTCAAGGTATCTGACGACGAAATAACGTCGATAGAAGATCTCAGCCAAGATGACTCTCCTGTGAGTCGCTTTATCAA
TCAGATACTGGTTGATGCGGTGCGTAAAGGCGCATCGGACATCCACTTCGAACCTTATGAAGAAAACTACCGCGTACGTC
TACGTTGCGATGGCATCCTTGTCGAGATCCAACAACCCGCCTCTCATTTAAGCCGTCGTCTGTCAGCTCGCTTAAAGATC
CTCGCTAAACTCGATATTGCCGAACGTCGCTTACCTCAAGATGGCCGTATCAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGCGTATCAACGCTGCCTACCTTATGGGGCGAGAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATAGACAAGCTCGGTTACAGCGAGGCTCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGACTTCGAGTACTCAACACCTCTGAACGCAA
CATCTCAACAGCTGAAGACCCAGTAGAGATTAACCTGTGTGGTATCAATCAGGTACAGGTGACGCCTAAGATTGGCTTTG
GTTTCGCCGAGGCACTACGATCGTTTTTACGACAAGACCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAAACC
GCCGAAATCGCGATCAAAGCCTCCCAAACCGGGCATTTAGTACTTTCAACACTGCACACTAACTCAGCCGCAGAAACCGT
GACCCGGCTTGCTCATATGGGAATCGAGCCCTTCAACCTTGCTTCGTCTTTGAGCTTGATTATTGCTCAGCGTCTTGCGA
GACGCTTGTGTAACCACTGTAAAACCGCAGACGACTCACCAGACATTTATCTGCGCCACTCTATCCCAAACAGCCAAACC
ATCTACAAAGCCAACTCTCAAGGCTGTAATGAATGTAACCAAGGTTACTCAGGTCGAGTCGGTATCTATGAAGTGATGCC
GTTTACCGACAAGCTAAAAACCAGCCTTATCAACAAACCCAATGCGCTAGCGATTGAAGATCTCGCGCGTAGAGAAGGCA
TGCGCACTCTGCAAGAGTCAGGGCTAGATAAACTGCTTGAAGGCACCACCAGCTATCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

74.113

100

0.745
  pilB Vibrio campbellii strain DS40M4

71.174

100

0.713
  pilB Vibrio parahaemolyticus RIMD 2210633

70.996

100

0.711
  pilB Legionella pneumophila strain ERS1305867

48.007

98.396

0.472
  pilB Acinetobacter baumannii D1279779

50.867

92.513

0.471
  pilB Acinetobacter baylyi ADP1

49.715

93.939

0.467
  pilF Neisseria gonorrhoeae MS11

44.464

99.822

0.444
  pilF Thermus thermophilus HB27

38.964

92.87

0.362