Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L0994_RS02595 Genome accession   NZ_CP090847
Coordinates   514165..514638 (-) Length   157 a.a.
NCBI ID   WP_016790756.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain WXL032     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 509165..519638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0994_RS02575 (L0994_02565) coaE 509683..510297 (-) 615 WP_010435920.1 dephospho-CoA kinase -
  L0994_RS02580 (L0994_02570) pilD 510300..511169 (-) 870 WP_373929936.1 A24 family peptidase Machinery gene
  L0994_RS02585 (L0994_02575) pilC 511236..512465 (-) 1230 WP_016768204.1 type II secretion system F family protein Machinery gene
  L0994_RS02590 (L0994_02580) pilB 512480..514165 (-) 1686 WP_065100288.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L0994_RS02595 (L0994_02585) pilA 514165..514638 (-) 474 WP_016790756.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  L0994_RS02600 (L0994_02590) nadC 514892..515782 (-) 891 WP_004738163.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L0994_RS02605 (L0994_02595) ampD 515866..516465 (+) 600 WP_016790757.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L0994_RS02610 (L0994_02600) pdhR 516947..517708 (+) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16745.10 Da        Isoelectric Point: 5.8402

>NTDB_id=645214 L0994_RS02595 WP_016790756.1 514165..514638(-) (pilA) [Vibrio cyclitrophicus strain WXL032]
MNNKSKRTNQKGFTLIELMIVVAIIGILSAFAVPAYQDYTKKATLSEFPKAAAAVKMAVELCAHESASDGDTFKTNCVST
TDNIPAAFDLNSMNILAIGGTSSGAVDVRVKATAIKGPIAANETYVMTATYTTNGITWESKCYIDAALNNQQETYCP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=645214 L0994_RS02595 WP_016790756.1 514165..514638(-) (pilA) [Vibrio cyclitrophicus strain WXL032]
ATGAATAACAAAAGTAAAAGAACAAATCAGAAAGGCTTTACGCTGATTGAATTGATGATTGTAGTGGCGATTATTGGGAT
CTTATCGGCGTTTGCTGTTCCTGCTTATCAAGATTACACGAAAAAAGCGACTCTATCCGAATTTCCGAAAGCAGCCGCTG
CTGTAAAAATGGCTGTTGAATTATGTGCACATGAAAGTGCTTCGGATGGTGATACATTTAAAACAAATTGTGTAAGTACT
ACCGACAATATTCCCGCAGCATTTGACCTTAACAGTATGAACATATTAGCTATTGGAGGCACCAGTTCAGGAGCTGTAGA
TGTAAGAGTCAAAGCGACTGCAATCAAAGGGCCTATTGCTGCAAATGAAACTTACGTAATGACAGCAACGTATACTACAA
ATGGCATCACGTGGGAGAGTAAGTGTTATATAGACGCAGCATTGAACAATCAACAAGAGACCTACTGCCCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

58.17

97.452

0.567
  pilA Haemophilus influenzae 86-028NP

39.333

95.541

0.376
  comP Acinetobacter baylyi ADP1

40.426

89.809

0.363