Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N4T39_RS20415 Genome accession   NZ_CP107720
Coordinates   4144291..4145676 (+) Length   461 a.a.
NCBI ID   WP_001025188.1    Uniprot ID   -
Organism   Escherichia coli strain 2021CK-01361     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4139291..4150676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4T39_RS20390 (N4T39_20390) aroP 4139839..4141212 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  N4T39_RS20395 (N4T39_20395) ampE 4141255..4142109 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  N4T39_RS20400 (N4T39_20400) ampD 4142106..4142657 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N4T39_RS20405 (N4T39_20405) nadC 4142745..4143638 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N4T39_RS20410 (N4T39_20410) pilA 4143841..4144281 (+) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  N4T39_RS20415 (N4T39_20415) pilB 4144291..4145676 (+) 1386 WP_001025188.1 type II secretion system protein GspE Machinery gene
  N4T39_RS20420 (N4T39_20420) hofC 4145666..4146868 (+) 1203 WP_000157277.1 protein transport protein HofC -
  N4T39_RS20425 (N4T39_20425) guaC 4146903..4147946 (-) 1044 WP_001217338.1 GMP reductase -
  N4T39_RS20430 (N4T39_20430) - 4148102..4148146 (-) 45 WP_175126954.1 hypothetical protein -
  N4T39_RS20435 (N4T39_20435) coaE 4148171..4148791 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  N4T39_RS20440 (N4T39_20440) zapD 4148791..4149534 (+) 744 WP_001194735.1 cell division protein ZapD -
  N4T39_RS20445 (N4T39_20445) yacG 4149544..4149741 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  N4T39_RS20450 (N4T39_20450) mutT 4149957..4150346 (-) 390 WP_000736009.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50617.30 Da        Isoelectric Point: 6.5786

>NTDB_id=644995 N4T39_RS20415 WP_001025188.1 4144291..4145676(+) (pilB) [Escherichia coli strain 2021CK-01361]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARHAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=644995 N4T39_RS20415 WP_001025188.1 4144291..4145676(+) (pilB) [Escherichia coli strain 2021CK-01361]
ATGAATATTCCACAGCTCACGGCCCTGTGCCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCAGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCATTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACTGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
TGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Legionella pneumophila strain ERS1305867

49.738

82.863

0.412
  pilB Vibrio campbellii strain DS40M4

48.705

83.731

0.408
  pilB Acinetobacter baylyi ADP1

39.957

100

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.667

84.599

0.395
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilF Neisseria gonorrhoeae MS11

44.675

83.514

0.373
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36