Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N4T39_RS20410 Genome accession   NZ_CP107720
Coordinates   4143841..4144281 (+) Length   146 a.a.
NCBI ID   WP_000360901.1    Uniprot ID   -
Organism   Escherichia coli strain 2021CK-01361     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4138841..4149281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4T39_RS20390 (N4T39_20390) aroP 4139839..4141212 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  N4T39_RS20395 (N4T39_20395) ampE 4141255..4142109 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  N4T39_RS20400 (N4T39_20400) ampD 4142106..4142657 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N4T39_RS20405 (N4T39_20405) nadC 4142745..4143638 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N4T39_RS20410 (N4T39_20410) pilA 4143841..4144281 (+) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  N4T39_RS20415 (N4T39_20415) pilB 4144291..4145676 (+) 1386 WP_001025188.1 type II secretion system protein GspE Machinery gene
  N4T39_RS20420 (N4T39_20420) hofC 4145666..4146868 (+) 1203 WP_000157277.1 protein transport protein HofC -
  N4T39_RS20425 (N4T39_20425) guaC 4146903..4147946 (-) 1044 WP_001217338.1 GMP reductase -
  N4T39_RS20430 (N4T39_20430) - 4148102..4148146 (-) 45 WP_175126954.1 hypothetical protein -
  N4T39_RS20435 (N4T39_20435) coaE 4148171..4148791 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15648.78 Da        Isoelectric Point: 4.3938

>NTDB_id=644994 N4T39_RS20410 WP_000360901.1 4143841..4144281(+) (pilA) [Escherichia coli strain 2021CK-01361]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQNDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=644994 N4T39_RS20410 WP_000360901.1 4143841..4144281(+) (pilA) [Escherichia coli strain 2021CK-01361]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCTGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAATGACAGCGCATTGCAGC
AAGCCTGTGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363