Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L0Z67_RS03455 Genome accession   NZ_CP090667
Coordinates   729284..730417 (+) Length   377 a.a.
NCBI ID   WP_062570118.1    Uniprot ID   A0AAQ1CBU1
Organism   Pseudoalteromonas sp. PB2-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 724284..735417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0Z67_RS03425 - 725015..725614 (-) 600 WP_062570112.1 XTP/dITP diphosphatase -
  L0Z67_RS03430 - 725622..726062 (-) 441 WP_062570113.1 DUF4426 domain-containing protein -
  L0Z67_RS03435 - 726117..726650 (-) 534 WP_062570114.1 YggT family protein -
  L0Z67_RS03440 proC 726674..727495 (-) 822 WP_062570115.1 pyrroline-5-carboxylate reductase -
  L0Z67_RS03445 - 727513..728226 (-) 714 WP_405128450.1 YggS family pyridoxal phosphate-dependent enzyme -
  L0Z67_RS03450 pilT 728229..729269 (+) 1041 WP_062570117.1 type IV pilus twitching motility protein PilT Machinery gene
  L0Z67_RS03455 pilU 729284..730417 (+) 1134 WP_062570118.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L0Z67_RS03460 - 730417..731286 (+) 870 WP_405128451.1 hypothetical protein -
  L0Z67_RS03465 ruvX 731283..731732 (-) 450 WP_193988351.1 Holliday junction resolvase RuvX -
  L0Z67_RS03470 - 731763..732320 (-) 558 WP_062570121.1 YqgE/AlgH family protein -
  L0Z67_RS03475 gshB 732345..733298 (-) 954 WP_193988352.1 glutathione synthase -
  L0Z67_RS03480 rsmE 733367..734101 (-) 735 WP_062570123.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L0Z67_RS03485 - 734161..734625 (-) 465 WP_062570124.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41784.75 Da        Isoelectric Point: 5.9727

>NTDB_id=644281 L0Z67_RS03455 WP_062570118.1 729284..730417(+) (pilU) [Pseudoalteromonas sp. PB2-1]
MTIPLNRFLQVMIDKNGSDLFVSSGLPVSAKINGELQPLTDDKLSDEQALAMVESAMNEKQRNEFHATKECNFAIATEEG
RFRVSAFWQRDQAGMVIRRIVTQIPDVNDLGLPSILTDVIMAKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIE
DPIEFVHEHQKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLVPTADGEGRVAAIEILLNSPMVAELIKRGDIGSIKETMAKSKEMGMQTFDQ
ALFELYKHQRINYADALHHADSPNDLRLMIKLQNNEQKGAGFLQGVTIDGLDEKGNS

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=644281 L0Z67_RS03455 WP_062570118.1 729284..730417(+) (pilU) [Pseudoalteromonas sp. PB2-1]
ATGACAATACCTTTAAATCGCTTTTTACAGGTGATGATCGATAAGAATGGTTCAGATTTATTTGTGTCTAGTGGCTTACC
TGTTAGTGCCAAAATTAACGGTGAATTGCAACCGCTGACCGACGATAAGTTATCTGATGAGCAGGCACTGGCTATGGTTG
AGTCTGCGATGAACGAAAAGCAACGTAATGAGTTTCACGCAACAAAAGAATGTAACTTTGCCATTGCAACAGAAGAAGGG
CGGTTTCGTGTTTCTGCATTTTGGCAGCGAGACCAAGCGGGTATGGTTATTCGCCGAATTGTGACGCAAATTCCTGATGT
AAACGATTTAGGATTACCGTCTATTCTTACCGATGTGATAATGGCAAAACGTGGCTTAGTGCTGTTTGTGGGGGGGACCG
GTACCGGTAAGTCAACCTCGCTTGCTGCGCTTTTAGGTTATCGAAATCGTAATCAACGTGGGCATATTCTTACCATTGAA
GATCCAATCGAGTTTGTGCATGAGCACCAAAAAAGTATTATTACTCAACGCGAAGTAGGTCTTGATACTGAAAGCTTTGA
GTCAGCGTTAAAAAGCTCATTACGCCAAGCGCCTGATGTTATTTTAATTGGTGAAATCCGTTCACAAGAAACCATGGAAT
ATGCATTAAGTTTTGCTGAAACAGGGCATTTATGTGTCGCAACGTTGCACGCTAATAATGCCAACCAAGCCATTGACCGT
ATTATGCACTTAGTACCAAAAGAAAAGCACGATAAGCTAAAATACGACTTAGCACTGAACCTACGCGCAATTGTTGCACA
GCAACTGGTGCCAACAGCAGATGGGGAAGGCCGTGTGGCTGCTATCGAGATCTTGCTTAATTCTCCTATGGTCGCTGAAC
TGATAAAGCGCGGTGATATTGGTTCAATAAAAGAAACTATGGCGAAATCAAAAGAAATGGGTATGCAGACTTTTGACCAA
GCCTTGTTTGAGCTTTATAAACACCAGCGTATTAATTATGCCGATGCCTTGCATCATGCTGACTCGCCAAACGATTTACG
CTTGATGATCAAGTTACAAAATAACGAGCAAAAAGGGGCTGGCTTTTTACAAGGTGTGACTATTGATGGCCTTGATGAAA
AGGGTAATAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.838

98.143

0.568
  pilU Acinetobacter baylyi ADP1

56.533

99.469

0.562
  pilU Vibrio cholerae strain A1552

56.403

97.347

0.549
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.056

95.491

0.411
  pilT Acinetobacter baumannii strain A118

41.088

87.798

0.361
  pilT Acinetobacter nosocomialis M2

41.088

87.798

0.361
  pilT Acinetobacter baumannii D1279779

41.088

87.798

0.361
  pilT Acinetobacter baylyi ADP1

40

90.186

0.361