Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LMJ37_RS10160 Genome accession   NZ_CP090523
Coordinates   2303505..2304635 (-) Length   376 a.a.
NCBI ID   WP_003489921.1    Uniprot ID   A0AAI7ZGP8
Organism   Xanthomonas citri pv. glycines strain ICMP5732     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2298505..2309635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMJ37_RS10135 (LMJ37_10135) ruvX 2299346..2299813 (-) 468 WP_016851093.1 Holliday junction resolvase RuvX -
  LMJ37_RS10140 (LMJ37_10140) - 2299806..2300372 (-) 567 WP_003489930.1 YqgE/AlgH family protein -
  LMJ37_RS10145 (LMJ37_10145) - 2300661..2301233 (+) 573 WP_016851094.1 DNA-3-methyladenine glycosylase I -
  LMJ37_RS10150 (LMJ37_10150) - 2301241..2302167 (+) 927 WP_016851095.1 DUF72 domain-containing protein -
  LMJ37_RS10155 (LMJ37_10155) - 2302342..2303016 (-) 675 WP_016851096.1 YitT family protein -
  LMJ37_RS10160 (LMJ37_10160) pilU 2303505..2304635 (-) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LMJ37_RS10165 (LMJ37_10165) pilT 2304758..2305795 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  LMJ37_RS10170 (LMJ37_10170) - 2306207..2306899 (+) 693 WP_016851097.1 YggS family pyridoxal phosphate-dependent enzyme -
  LMJ37_RS10175 (LMJ37_10175) proC 2306943..2307797 (+) 855 WP_011051928.1 pyrroline-5-carboxylate reductase -
  LMJ37_RS10180 (LMJ37_10180) - 2308357..2308782 (+) 426 WP_003489904.1 HU family DNA-binding protein -
  LMJ37_RS10185 (LMJ37_10185) - 2308889..2309530 (+) 642 WP_016851098.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42044.26 Da        Isoelectric Point: 6.8286

>NTDB_id=643215 LMJ37_RS10160 WP_003489921.1 2303505..2304635(-) (pilU) [Xanthomonas citri pv. glycines strain ICMP5732]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=643215 LMJ37_RS10160 WP_003489921.1 2303505..2304635(-) (pilU) [Xanthomonas citri pv. glycines strain ICMP5732]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATTACCCAGACCCCGCTGACCGCGCAGCAGAGTCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTTCGTGTCAGCTGCTTCTACCAGCGTAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAACTGAGCCTGCCGCCGGTGATCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGGACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGGCACATCATCACC
ATCGAAGATCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCATTCGCCGAAACCGGCCATCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGATCGCCGCAACCAGCTGTTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGACGGCCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATACGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAAATCAGTTACGAAGACGCGTTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAGGGCGGCGATGCCAAGACCTTGTCGCAGGGCCTGGACGGCGTGGAAATTTCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.457

94.947

0.394
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364