Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IHC87_RS00990 Genome accession   NZ_CP090501
Coordinates   205377..207104 (+) Length   575 a.a.
NCBI ID   WP_414477947.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain CSP DAM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 200377..212104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHC87_RS00970 (IHC87_00970) pilM 202606..203604 (+) 999 WP_237033307.1 type IV pilus biogenesis protein PilM -
  IHC87_RS00975 (IHC87_00975) - 203601..204242 (+) 642 WP_237029642.1 PilN domain-containing protein -
  IHC87_RS00980 (IHC87_00980) - 204239..204829 (+) 591 WP_068969671.1 type 4a pilus biogenesis protein PilO -
  IHC87_RS00985 (IHC87_00985) - 204838..205353 (+) 516 WP_237031270.1 pilus assembly protein PilP -
  IHC87_RS00990 (IHC87_00990) pilQ 205377..207104 (+) 1728 WP_414477947.1 type IV pilus secretin PilQ Machinery gene
  IHC87_RS00995 (IHC87_00995) aroK 207434..207952 (+) 519 WP_036765246.1 shikimate kinase AroK -
  IHC87_RS01000 (IHC87_01000) aroB 207982..209061 (+) 1080 WP_044174096.1 3-dehydroquinate synthase -
  IHC87_RS01005 (IHC87_01005) - 209076..210584 (+) 1509 WP_237033308.1 SPOR domain-containing protein -
  IHC87_RS01010 (IHC87_01010) dam 210692..211534 (+) 843 WP_005302664.1 adenine-specific DNA-methyltransferase -
  IHC87_RS01015 (IHC87_01015) - 211518..211715 (-) 198 WP_237033309.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62696.20 Da        Isoelectric Point: 4.9606

>NTDB_id=642964 IHC87_RS00990 WP_414477947.1 205377..207104(+) (pilQ) [Photobacterium damselae subsp. damselae strain CSP DAM1]
MKRMLSVSLGWQRCWVVMLCCWLGLSQAAIAAGMNIVNNQITDLDFKRDNRGRGVLTVQLAKPQSLVDMRRSGKQLHIDI
FDTAIKDDMLYVLDVVDFATVVRSIETFRTEKGSKLILNMAGVFDYDYQLKGSRLEVVLNAQKKKTSSTAKAKQGKIDYK
GKPISINFQDVPVRNVLQLVAEYNDFNLVVSDTVQGNVTLRLDDVPWPQVLDIILQSKGLDKRVQGSVVLIAPKSELATN
ERQEMEAKRKEDELASLRSELIQINYAKAGDIADLLLGTKDGVSMLSQRGSLHVDERTNAIIIKDIPESIGSIEEIITAL
DIPVKQVQIEARIVTVDEGDIDELGVRWGVVNQNGSTTIGGSIEGNLASSGLLGDDATVDDFLNVNLGAITPGASSIAFQ
VAKLGDILLDLELSALQAESKAEIISSPRLVTTNKKAAYIEQGTEIPYLEAASSGAVSVDFKKAVLSLMVTPQITPDDKL
VLDLLVTQDKVGETVVTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIYQHQVNNSVRKVPLLGDIPGLGVLFRHKLQEMG
KRELLIFVTPKIITQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=642964 IHC87_RS00990 WP_414477947.1 205377..207104(+) (pilQ) [Photobacterium damselae subsp. damselae strain CSP DAM1]
ATGAAAAGGATGTTATCGGTCAGCTTGGGTTGGCAACGTTGTTGGGTAGTTATGTTGTGCTGCTGGCTTGGTCTCTCCCA
AGCTGCCATTGCTGCTGGCATGAATATTGTGAATAACCAAATCACCGACTTAGATTTTAAGCGTGATAATCGTGGACGAG
GTGTGCTCACTGTTCAGTTAGCCAAACCTCAATCTCTGGTTGATATGCGCCGTTCAGGGAAGCAATTACATATCGATATC
TTTGATACCGCGATTAAAGATGACATGTTGTATGTTCTCGATGTAGTCGACTTTGCAACGGTTGTTCGTTCTATTGAAAC
ATTTCGCACCGAGAAAGGCAGTAAGCTGATTCTCAATATGGCTGGCGTGTTTGATTATGATTACCAATTAAAAGGCTCCC
GTCTTGAAGTTGTTTTGAACGCCCAGAAAAAGAAAACCAGTAGTACTGCCAAGGCTAAGCAAGGCAAAATTGATTATAAA
GGTAAGCCCATTTCCATTAACTTCCAAGATGTTCCTGTGCGTAACGTACTGCAGTTGGTTGCCGAATATAATGATTTTAA
TCTCGTGGTTTCCGATACCGTGCAGGGTAATGTGACCTTACGTCTTGATGATGTGCCTTGGCCACAAGTGCTCGATATTA
TTTTGCAATCAAAAGGGTTGGATAAACGAGTTCAAGGTTCTGTGGTACTGATTGCGCCAAAATCTGAGTTGGCAACCAAT
GAGCGTCAAGAGATGGAAGCTAAACGTAAAGAAGATGAGTTAGCTTCTCTTCGTTCGGAGCTGATTCAAATTAATTACGC
TAAAGCAGGAGATATCGCAGACCTTTTACTCGGAACGAAAGATGGGGTCAGTATGCTATCTCAACGTGGATCTTTGCATG
TTGATGAGCGTACTAATGCCATTATCATCAAAGATATCCCTGAGAGTATTGGTAGCATTGAAGAGATTATCACAGCATTA
GATATTCCCGTTAAACAGGTTCAGATAGAAGCTCGTATTGTTACTGTTGATGAGGGGGATATTGACGAGTTAGGGGTACG
TTGGGGTGTCGTTAATCAAAACGGTAGTACAACCATTGGTGGCTCAATTGAAGGTAACCTGGCCAGTTCGGGCTTATTGG
GTGATGACGCGACTGTTGATGATTTCTTAAATGTAAATTTAGGAGCCATTACTCCAGGGGCATCGAGTATTGCATTTCAG
GTGGCAAAACTGGGCGATATTTTGCTCGATCTTGAATTATCCGCTCTACAGGCAGAATCAAAAGCAGAGATTATTTCTAG
TCCTCGATTAGTGACTACCAATAAAAAAGCGGCCTACATCGAACAAGGTACTGAGATCCCTTATTTAGAAGCGGCGTCCA
GTGGTGCGGTATCGGTAGACTTTAAAAAGGCCGTATTGAGTTTGATGGTCACACCACAAATTACCCCAGATGATAAATTG
GTGCTCGATCTGCTGGTCACGCAAGATAAAGTAGGGGAAACAGTTGTGACCGGAACCGGAGAAGCGGTCGCTATTGATAC
TCAACGAATTGGTACGCAAGTTCTGGTCAATAACGGCGAGACTGTGGTCTTAGGTGGTATCTACCAGCATCAGGTGAATA
ATTCTGTCCGTAAAGTCCCTTTGTTGGGTGATATTCCAGGATTGGGCGTATTGTTCCGTCATAAGCTGCAAGAGATGGGC
AAGCGTGAACTGTTGATTTTCGTCACACCAAAAATTATCACCCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(415-573)

Secretin and TonB N terminus short domain (STN)

(186-234)

Bacterial type II/III secretion system short domain (Secretin_N)

(260-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

61.121

99.304

0.607
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

61.121

99.304

0.607
  pilQ Vibrio campbellii strain DS40M4

60.573

97.043

0.588
  pilQ Legionella pneumophila strain Lp02

42.647

94.609

0.403
  pilQ Legionella pneumophila strain ERS1305867

42.647

94.609

0.403
  pilQ Pseudomonas aeruginosa PAK

40.608

97.217

0.395
  comQ Acinetobacter baylyi ADP1

40.035

98.609

0.395
  pilQ Acinetobacter baumannii D1279779

38.966

100

0.393
  pilQ Acinetobacter baumannii strain A118

38.966

100

0.393