Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IHC89_RS01055 Genome accession   NZ_CP090495
Coordinates   221060..222787 (+) Length   575 a.a.
NCBI ID   WP_370469523.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain AS-15-0759-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 216060..227787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHC89_RS01035 (IHC89_01035) pilM 218289..219287 (+) 999 WP_068945319.1 type IV pilus biogenesis protein PilM -
  IHC89_RS01040 (IHC89_01040) - 219284..219925 (+) 642 WP_068945318.1 PilN domain-containing protein -
  IHC89_RS01045 (IHC89_01045) - 219922..220512 (+) 591 WP_237031269.1 type 4a pilus biogenesis protein PilO -
  IHC89_RS01050 (IHC89_01050) - 220521..221036 (+) 516 WP_237031270.1 pilus assembly protein PilP -
  IHC89_RS01055 (IHC89_01055) pilQ 221060..222787 (+) 1728 WP_370469523.1 type IV pilus secretin PilQ Machinery gene
  IHC89_RS01060 (IHC89_01060) aroK 223117..223635 (+) 519 WP_036765246.1 shikimate kinase AroK -
  IHC89_RS01065 (IHC89_01065) aroB 223665..224744 (+) 1080 WP_005302661.1 3-dehydroquinate synthase -
  IHC89_RS01070 (IHC89_01070) - 224759..226267 (+) 1509 WP_237031271.1 SPOR domain-containing protein -
  IHC89_RS01075 (IHC89_01075) dam 226375..227217 (+) 843 WP_065172417.1 adenine-specific DNA-methyltransferase -
  IHC89_RS01080 (IHC89_01080) - 227201..227398 (-) 198 WP_237031272.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62710.18 Da        Isoelectric Point: 4.9002

>NTDB_id=642894 IHC89_RS01055 WP_370469523.1 221060..222787(+) (pilQ) [Photobacterium damselae subsp. damselae strain AS-15-0759-2]
MKRMLSVSLGWQRCWVVMLCCWLGLSQAAIAAGMNIVNNQITDLDFKRDNRGRGVLTVQLAKPQSLVDMRRSGKQLHIDI
FDTAIKDDMLYVLDVVDFATVVRSIETFRTEKGSKLILNMAGVFDYDYQLKGSRLEVVLNAQKKKASSTAKAKQGKIDYK
GKPISINFQDVPVRNVLQLVAEYNDFNLVVSDTVQGNVTLRLDDVPWPQVLDIILQSKGLDKRVQGSVVLIAPKSELATN
ERQEMEAKRKEDELASLRSELIQINYAKAGDIADLLLGTKDGVSMLSQRGSLHVDERTNAIIIKDIPESIDSIEDIITAL
DIPVKQVQIEARIVTVDEGDIDELGVRWGVVNQNGSTTIGGSIEGNLASSGLLGDDATVDDFLNVNLGAITPGASSIAFQ
VAKLGDILLDLELSALQAESKAEIISSPRLVTTNKKAAYIEQGTEIPYLEAASSGAVSVDFKKAVLSLMVTPQITPDDKL
VLDLLVTQDKVGETVVTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIYQHQVNNSVRKVPLLGDIPGLGVLFRHKLQEMG
KRELLIFVTPKIITQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=642894 IHC89_RS01055 WP_370469523.1 221060..222787(+) (pilQ) [Photobacterium damselae subsp. damselae strain AS-15-0759-2]
ATGAAAAGGATGTTATCGGTCAGCTTGGGTTGGCAACGTTGTTGGGTAGTTATGTTGTGCTGCTGGCTTGGTCTCTCCCA
AGCTGCCATTGCTGCTGGCATGAATATTGTGAATAACCAAATCACCGACTTAGATTTTAAGCGTGATAATCGTGGACGAG
GCGTGCTCACTGTTCAGTTAGCCAAACCTCAATCTCTGGTTGATATGCGCCGTTCAGGGAAGCAATTACATATCGATATC
TTTGATACCGCGATTAAAGATGACATGTTGTATGTTCTCGATGTAGTCGACTTTGCAACGGTTGTCCGTTCGATTGAAAC
ATTTCGCACCGAGAAAGGCAGCAAGCTTATTCTCAATATGGCTGGCGTATTTGATTATGATTACCAATTAAAAGGCTCTC
GTCTTGAAGTTGTTTTGAACGCCCAGAAAAAGAAAGCCAGCAGTACAGCCAAGGCTAAGCAAGGCAAAATTGATTATAAA
GGTAAGCCCATCTCCATTAACTTCCAAGATGTTCCTGTGCGTAACGTATTGCAGTTGGTTGCCGAATATAATGATTTTAA
TCTCGTGGTTTCCGATACTGTGCAGGGTAATGTGACCTTACGTCTTGATGATGTGCCTTGGCCACAAGTGCTCGATATTA
TTTTGCAATCAAAAGGGTTGGATAAACGAGTTCAAGGTTCTGTGGTACTGATTGCGCCAAAATCTGAGTTGGCAACCAAT
GAGCGTCAAGAGATGGAAGCCAAACGTAAAGAAGATGAGTTAGCTTCGCTTCGTTCGGAGCTGATTCAAATTAATTACGC
TAAAGCAGGAGATATCGCAGACCTTTTACTCGGAACGAAAGATGGAGTCAGTATGCTATCTCAACGCGGGTCTTTGCATG
TTGATGAGCGTACTAATGCCATAATCATTAAAGATATTCCTGAGAGTATTGATAGCATTGAAGATATTATCACGGCACTA
GATATTCCCGTTAAACAGGTTCAGATAGAAGCTCGTATTGTTACTGTTGATGAAGGGGATATTGACGAGTTAGGGGTACG
TTGGGGTGTCGTTAATCAAAACGGTAGTACAACCATTGGGGGCTCAATTGAAGGTAACTTGGCCAGTTCGGGCTTATTGG
GTGATGACGCGACTGTTGATGATTTCTTAAATGTAAATTTAGGTGCAATTACTCCTGGGGCATCGAGTATTGCATTTCAG
GTGGCAAAACTGGGCGATATTTTGCTCGATCTTGAATTATCCGCTCTACAGGCAGAATCGAAAGCAGAGATTATTTCTAG
TCCTCGATTAGTAACCACCAATAAAAAAGCGGCTTACATCGAACAAGGTACAGAGATCCCTTATTTAGAAGCGGCATCTA
GTGGTGCGGTATCGGTAGACTTTAAAAAGGCCGTATTGAGCTTGATGGTCACACCACAAATTACCCCTGATGATAAATTG
GTGCTCGATCTGCTGGTAACGCAAGATAAAGTAGGGGAAACAGTTGTTACCGGAACCGGAGAAGCGGTCGCTATCGATAC
TCAACGAATTGGCACGCAAGTTCTGGTCAATAATGGCGAGACTGTAGTCTTAGGGGGTATCTATCAGCATCAGGTGAATA
ATTCTGTCCGTAAAGTCCCTTTGTTGGGCGATATTCCAGGATTGGGCGTATTGTTCCGTCATAAGCTGCAAGAGATGGGC
AAGCGTGAACTGTTGATTTTCGTCACACCCAAAATTATCACCCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-326)

Secretin and TonB N terminus short domain (STN)

(186-234)

Bacterial type II and III secretion system protein (Secretin)

(415-573)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

61.121

99.304

0.607
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

61.121

99.304

0.607
  pilQ Vibrio campbellii strain DS40M4

60.932

97.043

0.591
  pilQ Legionella pneumophila strain Lp02

42.647

94.609

0.403
  pilQ Legionella pneumophila strain ERS1305867

42.647

94.609

0.403
  pilQ Pseudomonas aeruginosa PAK

41.103

97.739

0.402
  comQ Acinetobacter baylyi ADP1

40.388

98.609

0.398
  pilQ Acinetobacter baumannii D1279779

39.138

100

0.395
  pilQ Acinetobacter baumannii strain A118

39.138

100

0.395