Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LZG54_RS12165 Genome accession   NZ_CP090382
Coordinates   2533685..2535397 (+) Length   570 a.a.
NCBI ID   WP_234114090.1    Uniprot ID   -
Organism   Acinetobacter towneri strain SCLZS30     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2528685..2540397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZG54_RS12125 (LZG54_12140) nusA 2529214..2530698 (-) 1485 WP_234114087.1 transcription termination factor NusA -
  LZG54_RS12130 (LZG54_12145) rimP 2530734..2531258 (-) 525 WP_234114088.1 ribosome maturation factor RimP -
  LZG54_RS12155 (LZG54_12170) secG 2532273..2532602 (-) 330 WP_004975868.1 preprotein translocase subunit SecG -
  LZG54_RS12160 (LZG54_12175) tpiA 2532616..2533407 (-) 792 WP_234114089.1 triose-phosphate isomerase -
  LZG54_RS12165 (LZG54_12180) pilB 2533685..2535397 (+) 1713 WP_234114090.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LZG54_RS12170 (LZG54_12185) pilC 2535428..2536654 (+) 1227 WP_004975864.1 type II secretion system F family protein Machinery gene
  LZG54_RS14710 pilD 2536654..2537514 (+) 861 WP_267970595.1 prepilin peptidase Machinery gene
  LZG54_RS12185 (LZG54_12200) coaE 2537524..2538123 (+) 600 WP_004975860.1 dephospho-CoA kinase -
  LZG54_RS12190 (LZG54_12205) - 2538129..2539007 (-) 879 WP_234115030.1 DMT family transporter -
  LZG54_RS12195 (LZG54_12210) rlmB 2539102..2539851 (-) 750 WP_234114091.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LZG54_RS12200 (LZG54_12215) - 2540039..2540362 (-) 324 WP_004975854.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63514.73 Da        Isoelectric Point: 4.8163

>NTDB_id=641888 LZG54_RS12165 WP_234114090.1 2533685..2535397(+) (pilB) [Acinetobacter towneri strain SCLZS30]
MSVQQATPRFTGFIRRLVEDGLVSAENMQTAIMAAHKDNVDIVPYLIQNLNISPLLIAEKISNEFGEPIFDLSVYDSALI
VREGIDEKLFSKYCLLPIFKRNHLLYVATSNPTNIEAMDAIRFNSKMNIEAIVVEHDKLVKLIEQNFASESAFEFEDDDF
DLNFEEGNSEPEQAAEDEPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKNKSIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKAPSDIPKQSLLEMGF
TEADLSNPEFQVYQPVGCSECREGYKGRVGVYEVMKVTPEISRIIMEDGNAIQIADASAKSGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=641888 LZG54_RS12165 WP_234114090.1 2533685..2535397(+) (pilB) [Acinetobacter towneri strain SCLZS30]
ATGTCAGTACAACAAGCTACACCTAGATTTACAGGTTTTATTCGACGCTTGGTCGAAGATGGGCTCGTTTCTGCTGAAAA
TATGCAAACTGCCATTATGGCCGCTCACAAAGATAATGTTGATATTGTTCCCTATCTCATTCAAAACCTAAATATTTCAC
CTCTGCTGATTGCTGAAAAAATTTCTAATGAGTTTGGCGAACCTATTTTTGACTTGAGTGTCTATGACTCCGCTTTAATC
GTGCGTGAAGGCATTGATGAAAAACTGTTCAGCAAATATTGTCTATTGCCTATTTTTAAACGCAATCATCTGCTTTATGT
TGCAACCAGTAACCCGACCAATATCGAAGCAATGGATGCCATTCGTTTCAACAGTAAAATGAATATTGAAGCCATTGTGG
TCGAACACGATAAATTGGTAAAACTGATTGAACAAAACTTTGCGAGTGAGTCTGCTTTTGAATTTGAAGATGATGACTTC
GATCTCAACTTTGAAGAGGGTAACAGCGAACCAGAACAAGCTGCAGAAGATGAACCGCAGGGTGATGAATCCCCAATTGT
TAAATACATCAACAAATTACTGATTGATGCAATTCGCATGGGTGCTTCGGACTTACACTTTGAACCGTATGAAAAAACCT
ATCGAGTGCGTTACCGTGTCGATGGTGTATTACGTCAAATTGCCACCCCACCCTTACAGTTAGCTAACCGCTTGGCATCA
CGTTTAAAAGTGATGTCCCAAATGGACATTTCTGAAAAGCGTGTCCCGCAAGATGGTCGTATTAAATTAAAACTATCTAA
AAATAAATCCATCGACTTCCGTGTGAACTCGTTGCCAACATTGTTCGGTGAAAAAATCGTACTGCGTATTCTTGACCCAT
CTAGCGCGATGCTTGGGATTGACGCTTTGGGCTATGAACCAGAACAAAAAGAATTGTTCATGGAAGCCTTAGAAAAACCG
CAAGGCATGTTACTGATTACAGGTCCAACGGGTTCGGGTAAAACGGTATCGCTATATACAGGCTTAAATATCTTAAACAG
AGAAGATACCAACATTTCCACTGCCGAAGACCCTGTGGAAATTAACTTAGAAGGCATCAACCAGGTTAACGTCAACAATA
AAGTGGGCTTAACCTTCGCTGCCGCTCTGAAATCTTTCTTACGTCAAGACCCTGATATTGTCATGGTGGGTGAGATTCGT
GACCTTGAAACTGCAGAAATTGCAATTAAAGCAGCCCAAACAGGTCACATGGTTTTATCTACCCTCCACACCAACAGTGC
GCCAGAAACTTTAACGCGTTTACGCAATATGGGCGTTCCTTCCTTTAACATCGCAACTTCGGTAAACCTCGTCATTGCAC
AGCGTTTGGCACGACGCCTATGCCCGCATTGCAAAGCTCCTTCCGATATTCCAAAACAAAGCTTATTGGAAATGGGCTTT
ACTGAAGCCGATTTGAGCAATCCAGAATTTCAGGTCTATCAACCTGTAGGCTGTTCTGAATGCCGTGAAGGTTATAAAGG
ACGTGTTGGTGTTTATGAAGTGATGAAAGTCACACCAGAAATTTCTAGAATTATTATGGAGGATGGTAATGCCATCCAAA
TTGCTGATGCGTCAGCAAAATCGGGGTTTAACAATCTGCGTCGTTCGGGGTTACTTAAGGTCATGCAGGGGGTGACTTCA
TTGCAAGAAGTCAATCGTGTGACCAGTGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

78.421

100

0.784
  pilB Acinetobacter baylyi ADP1

76.316

100

0.763
  pilB Legionella pneumophila strain ERS1305867

52.593

94.737

0.498
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio cholerae strain A1552

48.587

99.298

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

48.393

98.246

0.475
  pilB Vibrio campbellii strain DS40M4

50.289

91.053

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.484

100

0.386
  pilF Thermus thermophilus HB27

37.435

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.388

98.07

0.367