Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LXM48_RS13480 Genome accession   NZ_CP090180
Coordinates   2751820..2753532 (+) Length   570 a.a.
NCBI ID   WP_234304143.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain DJ-Red     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2746820..2758532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXM48_RS13435 (LXM48_13435) nusA 2747405..2748889 (-) 1485 WP_078425744.1 transcription termination factor NusA -
  LXM48_RS13440 (LXM48_13440) rimP 2748929..2749453 (-) 525 WP_004692708.1 ribosome maturation factor RimP -
  LXM48_RS13470 (LXM48_13470) secG 2750405..2750734 (-) 330 WP_078388529.1 preprotein translocase subunit SecG -
  LXM48_RS13475 (LXM48_13475) tpiA 2750748..2751539 (-) 792 WP_078425745.1 triose-phosphate isomerase -
  LXM48_RS13480 (LXM48_13480) pilB 2751820..2753532 (+) 1713 WP_234304143.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LXM48_RS13485 (LXM48_13485) pilC 2753563..2754789 (+) 1227 WP_234304144.1 type II secretion system F family protein Machinery gene
  LXM48_RS13490 (LXM48_13490) pilD 2754789..2755649 (+) 861 WP_058868793.1 A24 family peptidase Machinery gene
  LXM48_RS13495 (LXM48_13495) coaE 2755658..2756257 (+) 600 WP_234304145.1 dephospho-CoA kinase -
  LXM48_RS13500 (LXM48_13500) - 2756272..2757189 (-) 918 Protein_2615 DMT family transporter -
  LXM48_RS13505 (LXM48_13505) rlmB 2757222..2757971 (-) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LXM48_RS13510 (LXM48_13510) - 2758114..2758437 (-) 324 WP_078388524.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63593.92 Da        Isoelectric Point: 4.9493

>NTDB_id=641029 LXM48_RS13480 WP_234304143.1 2751820..2753532(+) (pilB) [Acinetobacter johnsonii strain DJ-Red]
MSVSNGLPKFTGFIRRLVEDGRMTAENMQQAMLSAKKAQQDIVPYLIENYKLSSQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKAYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=641029 LXM48_RS13480 WP_234304143.1 2751820..2753532(+) (pilB) [Acinetobacter johnsonii strain DJ-Red]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGGCGCATGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATTGTTCCTTATCTAATTGAAAATTATAAACTCTCCT
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGAGAGCCTGTTTTTGACTTAAGTGTCTATGATTCGGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTCATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCCACCAGCAATCCAACCAACATGGATGCGATGGACGCGATTCGCTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAACATCCCCAGCTTGAAAAACTGATCGAACAAAATTTTGCACAAGCCGATAGTTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCAAATGACGAAGAAGATGACTCACCTCAAGGGGATGAAGCTCCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCCTCAGACTTACACTTTGAGCCTTATGAAAAGGCTT
ACCGTGTTCGTTATCGTGTAGATGGGGTTCTGCGTCATATTGCCAGCCCACCACTACAACTGGCGACTCGTCTTGCTTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGCCGGATTAAATTAAAGCTGTCAAA
AAGTAAAGCCATCGACTTTCGTGTCAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CCAGTGCTATGCTGGGAATTGATGCTTTAGGTTATGAACCAGACCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCC
CAAGGCATGTTGTTAATTACTGGCCCGACAGGTTCTGGTAAAACGGTATCGCTATATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATTTCAACTGCGGAAGACCCTGTTGAGATTAACTTAGAAGGTATTAATCAGGTCAATGTGAACAATA
AAGTCGGCCTCACCTTCTCTGCTGCGCTGAAGTCATTCTTACGTCAAGATCCTGATATTGTGATGGTGGGTGAGATTCGT
GACCTTGAAACAGCTGAAATCGCGATTAAAGCCGCGCAGACAGGTCATATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACCCGCTTACGCAATATGGGGGTTCCATCTTTTAACATTGCGACCTCGGTCAATTTGGTGATTGCAC
AACGTTTAGCACGCCGCTTATGCAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGATCCAGATTTCAAAGTTTATCAACCGGTGGGGTGTGCTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.877

100

0.809
  pilB Acinetobacter baylyi ADP1

76.316

100

0.763
  pilB Legionella pneumophila strain ERS1305867

53.933

93.684

0.505
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio cholerae strain A1552

48.677

99.474

0.484
  pilB Vibrio campbellii strain DS40M4

48.446

95.965

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

50

92.632

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

33.981

100

0.368
  pilF Thermus thermophilus HB27

36.427

100

0.365