Detailed information NTG0641000

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: LXM48_RS00480
• Genome accession: NZ_CP090180
• Coordinates: 96937..97995 (+)
• Length: 352 a.a.
• NCBI ID: WP_058870420.1
• Uniprot ID: A0A345B4L2
• Organism: Acinetobacter johnsonii strain DJ-Red
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 91937..102995

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LXM48_RS00465 (LXM48_00465)	mraY	92217..93335 (+)	1119	WP_004691502.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
LXM48_RS00470 (LXM48_00470)	-	93405..94211 (+)	807	WP_125280896.1	putative RNA methyltransferase	-
LXM48_RS00475 (LXM48_00475)	ponA	94243..96771 (-)	2529	WP_125280897.1	penicillin-binding protein PBP1a	-
LXM48_RS00480 (LXM48_00480)	pilM	96937..97995 (+)	1059	WP_058870420.1	pilus assembly protein PilM	Machinery gene
LXM48_RS00485 (LXM48_00485)	pilN	97995..98651 (+)	657	WP_004691498.1	PilN domain-containing protein	Machinery gene
LXM48_RS00490 (LXM48_00490)	pilO	98648..99370 (+)	723	WP_234304417.1	type 4a pilus biogenesis protein PilO	Machinery gene
LXM48_RS00495 (LXM48_00495)	pilP	99370..99897 (+)	528	WP_004691496.1	pilus assembly protein PilP	Machinery gene
LXM48_RS00500 (LXM48_00500)	pilQ	99917..101995 (+)	2079	WP_234304418.1	type IV pilus secretin PilQ family protein	Machinery gene
LXM48_RS00505 (LXM48_00505)	-	102084..102566 (+)	483	WP_234305130.1	shikimate kinase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38953.50 Da
• Isoelectric Point: 4.4181

>NTDB_id=641000 LXM48_RS00480 WP_058870420.1 96937..97995(+) (pilM) [Acinetobacter johnsonii strain DJ-Red]
MLRLYRKPSKGLIGVDISSTSVKVLELSVKNNRYWVESYALVPLPEGSVVEKNILNPEAVGDALERAVNLANVQSTDVAL
AVPTSMVITKIIEMDADMNDDEREIQIRDDAEQYIPFPLDEASLDFEVLPDRLANPNRVNVLLVATRIENVEARSEALEL
GGLTPKIADVESFAIENAFKVFSDTLPMGVNTVGILDIGHTMTTLSVMQNNKVIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDIEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=641000 LXM48_RS00480 WP_058870420.1 96937..97995(+) (pilM) [Acinetobacter johnsonii strain DJ-Red]
GTGCTCAGGTTATATCGTAAGCCAAGTAAGGGGTTAATAGGAGTAGATATTAGTTCGACTTCTGTTAAGGTATTGGAACT
TTCTGTTAAAAACAATCGGTATTGGGTGGAGAGCTATGCACTAGTCCCATTACCAGAAGGAAGTGTGGTCGAAAAAAATA
TATTGAATCCAGAAGCGGTTGGTGATGCCCTTGAGCGTGCAGTAAATTTGGCCAATGTTCAATCGACAGACGTAGCACTG
GCAGTTCCGACTTCCATGGTGATTACTAAAATCATTGAGATGGATGCCGATATGAACGATGACGAACGTGAAATTCAGAT
TCGTGATGATGCTGAGCAATATATTCCATTCCCATTAGATGAAGCGAGTTTGGACTTTGAAGTCCTTCCAGACCGTTTAG
CCAATCCCAATCGTGTCAATGTACTCCTTGTTGCAACCCGCATTGAAAATGTAGAGGCGCGTTCCGAAGCATTGGAGTTA
GGTGGTTTAACGCCTAAGATTGCCGATGTTGAAAGTTTCGCCATTGAAAATGCATTCAAAGTCTTTTCAGATACGCTTCC
AATGGGTGTAAACACCGTTGGGATTCTGGACATCGGTCATACCATGACCACCTTATCTGTCATGCAGAATAATAAAGTCA
TTTATACCCGTGAGCAGGTGTTCGGTGGTAAACAACTGACTCAAGAAATTCAAAACCGTTATGGTTTGTCTTTTGAAGAA
GCTGGACGTGCCAAAAAAAGCCGTACCTTGCCTGACGACTATGATATTGAAGTCCTTGAACCTTTTCTTGAAGCTGTGGT
GCAACAAGCAGCACGTTCTTTGCAGTTCTTCTTTTCGTCTTCTCAATTCAATGAGATCGACCATATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGACTGGCGAAGTTATTACAACAAAAATTAGGTTATCGGGTCACTATTGCCAACCCATTTTTA
CAGATGGGCTTTTCTCCACAAATCGACATTAAAAAAATTGAAAATGATGCTTCATCACTCATGGTGGCATGTGGATTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A345B4L2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.227	100	0.852
comM	Acinetobacter nosocomialis M2	85.227	100	0.852
comM	Acinetobacter baylyi ADP1	80.398	100	0.804
pilM	Legionella pneumophila strain ERS1305867	40.96	100	0.412