Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   U771_RS05040 Genome accession   NC_023064
Coordinates   1127002..1128210 (-) Length   402 a.a.
NCBI ID   WP_024073384.1    Uniprot ID   -
Organism   Pseudomonas sp. TKP     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1122002..1133210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U771_RS05000 (U771_05100) - 1122887..1123060 (-) 174 WP_024073379.1 DUF3094 family protein -
  U771_RS05005 (U771_05105) - 1123150..1123779 (-) 630 WP_017845475.1 DUF1780 domain-containing protein -
  U771_RS05010 (U771_05110) - 1123880..1124347 (+) 468 WP_024073380.1 hypothetical protein -
  U771_RS05015 (U771_05115) - 1124344..1125033 (+) 690 WP_024073381.1 energy-coupling factor ABC transporter permease -
  U771_RS05020 (U771_05120) - 1125110..1125325 (+) 216 WP_003171680.1 hypothetical protein -
  U771_RS05025 (U771_05125) yacG 1125322..1125522 (-) 201 WP_017845472.1 DNA gyrase inhibitor YacG -
  U771_RS05030 (U771_05130) coaE 1125519..1126142 (-) 624 WP_024073382.1 dephospho-CoA kinase -
  U771_RS05035 (U771_05135) pilD 1126139..1127005 (-) 867 WP_024073383.1 prepilin peptidase Machinery gene
  U771_RS05040 (U771_05140) pilC 1127002..1128210 (-) 1209 WP_024073384.1 type II secretion system F family protein Machinery gene
  U771_RS05045 (U771_05145) - 1128412..1128825 (+) 414 WP_024073385.1 pilin -
  U771_RS05050 (U771_05150) - 1128860..1130269 (+) 1410 WP_024073386.1 O-antigen ligase family protein -
  U771_RS05060 (U771_05160) - 1130532..1130837 (+) 306 WP_024073387.1 DUF6388 family protein -
  U771_RS05065 (U771_05165) nadC 1130959..1131807 (-) 849 WP_024073388.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43372.75 Da        Isoelectric Point: 10.2419

>NTDB_id=64095 U771_RS05040 WP_024073384.1 1127002..1128210(-) (pilC) [Pseudomonas sp. TKP]
MDNASRTYAWEGTNRQGRRVSGHTTGHNLALIKAKLRQQGIRPSRVRRQLPGLACLAAPIKPTDIALLTRQLATLLKAGI
PVLQAFDIISDGFESRPMQELLKGLKQDIAAGTALATALRKQPRYFDDLYCNLIAAGEQAGALETLLERVATHLEKSEQL
KARIKKAMTYPIAVLVVASLVSSILLIHVVPQFQNLFAGVDRQLPGFTLHVIGLSTFMQQAWWALALGLGAGFFALRHAY
RVAPGFRQGLDTGLLKAPLAGTLLKKSAVARYARTLSTTFAAGVPLVQALDSVAGAVGNGPYKQAIKHMRHEVSTGMQLN
QSMAASGLFPGMAIQMTAIGEESGTLERMLEKVANHYESEVDNLVDNLTSLMEPLIMVVLGGIVGALVIAMYLPVFQLGT
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=64095 U771_RS05040 WP_024073384.1 1127002..1128210(-) (pilC) [Pseudomonas sp. TKP]
ATGGACAACGCTTCAAGGACCTACGCCTGGGAAGGCACCAACCGCCAAGGGCGCAGGGTGTCCGGGCACACCACGGGCCA
CAACCTCGCCCTGATCAAGGCCAAGCTGCGCCAGCAAGGGATCCGCCCCAGCCGTGTGCGCAGGCAACTCCCCGGCCTGG
CCTGCCTCGCCGCGCCGATCAAACCCACTGACATCGCCCTGCTCACCCGCCAGTTGGCGACCTTGCTGAAAGCCGGCATT
CCTGTGCTGCAAGCCTTCGATATCATCAGCGACGGCTTCGAGAGCCGGCCGATGCAGGAACTGCTCAAGGGGTTGAAGCA
GGACATCGCCGCCGGCACCGCACTGGCGACCGCGCTGCGCAAACAGCCACGCTATTTCGATGACCTGTACTGCAACCTGA
TTGCCGCCGGCGAACAGGCCGGGGCATTGGAAACGCTGCTGGAACGCGTTGCCACTCACCTGGAAAAGAGTGAGCAGCTA
AAGGCGCGAATCAAGAAAGCCATGACCTACCCGATCGCCGTCCTGGTGGTGGCGAGCCTGGTCAGCAGCATCCTGCTGAT
CCACGTGGTGCCGCAATTTCAGAACCTGTTCGCCGGGGTCGACCGCCAGTTGCCCGGTTTTACCCTGCACGTCATCGGCC
TGTCCACGTTCATGCAGCAAGCCTGGTGGGCTCTGGCGCTTGGCCTCGGGGCCGGTTTTTTCGCGCTGCGCCACGCCTAT
CGCGTAGCCCCTGGCTTTCGTCAGGGGCTGGATACGGGTTTGTTGAAAGCCCCCTTGGCAGGCACACTGCTGAAGAAATC
GGCCGTGGCACGCTATGCCCGCACGCTTTCCACCACGTTCGCGGCTGGGGTGCCGCTGGTACAGGCATTGGATTCAGTGG
CAGGTGCCGTCGGCAACGGGCCGTACAAGCAGGCGATCAAACATATGCGTCATGAGGTATCCACAGGCATGCAGTTGAAC
CAATCCATGGCCGCCAGCGGTCTCTTTCCAGGCATGGCAATTCAGATGACGGCCATCGGCGAGGAGTCGGGCACACTCGA
GCGCATGCTGGAAAAAGTCGCGAACCACTATGAATCGGAAGTCGACAACCTGGTCGACAACCTCACCAGCCTGATGGAGC
CGCTGATAATGGTGGTGCTGGGGGGCATTGTCGGGGCGCTGGTAATTGCGATGTACCTGCCGGTCTTCCAACTCGGTACA
GCGTTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.071

98.507

0.562
  pilC Acinetobacter baylyi ADP1

51.768

98.507

0.51
  pilC Acinetobacter baumannii D1279779

50.251

99.005

0.498
  pilC Legionella pneumophila strain ERS1305867

48.99

98.507

0.483
  pilC Vibrio cholerae strain A1552

40.602

99.254

0.403
  pilC Vibrio campbellii strain DS40M4

38.191

99.005

0.378
  pilG Neisseria gonorrhoeae MS11

38.287

98.756

0.378
  pilC Thermus thermophilus HB27

37.656

99.751

0.376
  pilG Neisseria meningitidis 44/76-A

37.879

98.507

0.373

Multiple sequence alignment