Detailed information NTG0638441

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ND010_RS10265
• Genome accession: NZ_CP106826
• Coordinates: 1951779..1953005 (-)
• Length: 408 a.a.
• NCBI ID: WP_047920901.1
• Uniprot ID: -
• Organism: Neisseria gonorrhoeae strain 9464
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1946779..1958005

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ND010_RS10255 (ND010_10255)	-	1948553..1948837 (+)	285	WP_003692093.1	GIY-YIG nuclease family protein	-
ND010_RS10260 (ND010_10260)	yccS	1949512..1951662 (-)	2151	WP_003697785.1	YccS family putative transporter	-
ND010_RS10265 (ND010_10265)	pilU	1951779..1953005 (-)	1227	WP_047920901.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ND010_RS10270 (ND010_10270)	pilT	1953169..1954212 (-)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
ND010_RS10275 (ND010_10275)	-	1954332..1955018 (+)	687	WP_003690201.1	YggS family pyridoxal phosphate-dependent enzyme	-
ND010_RS10280 (ND010_10280)	-	1955018..1955425 (+)	408	WP_235220100.1	RNA-binding protein	-
ND010_RS10285 (ND010_10285)	proC	1955474..1956265 (+)	792	WP_260245430.1	pyrroline-5-carboxylate reductase	-
ND010_RS10290 (ND010_10290)	dksA	1956414..1956830 (+)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45670.27 Da
• Isoelectric Point: 7.0921

>NTDB_id=638441 ND010_RS10265 WP_047920901.1 1951779..1953005(-) (pilU) [Neisseria gonorrhoeae strain 9464]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGTHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=638441 ND010_RS10265 WP_047920901.1 1951779..1953005(-) (pilU) [Neisseria gonorrhoeae strain 9464]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCACACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTGGGCTTACCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGATCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387