Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   LVC68_RS15685 Genome accession   NZ_CP089742
Coordinates   3387957..3388484 (+) Length   175 a.a.
NCBI ID   WP_269783622.1    Uniprot ID   -
Organism   Melaminivora jejuensis strain KACC 19629     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 3386156..3387412 3387957..3388484 flank 545


Gene organization within MGE regions


Location: 3386156..3388484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVC68_RS15670 (LVC68_15670) - 3386156..3387412 (+) 1257 WP_209423586.1 RNA-guided endonuclease TnpB family protein -
  LVC68_RS15675 (LVC68_15675) - 3387490..3387999 (+) 510 WP_232653791.1 hypothetical protein -
  LVC68_RS15685 (LVC68_15685) pilE 3387957..3388484 (+) 528 WP_269783622.1 pilin Machinery gene

Sequence

Protein


Download         Length: 175 a.a.        Molecular weight: 17959.53 Da        Isoelectric Point: 6.2693

>NTDB_id=638416 LVC68_RS15685 WP_269783622.1 3387957..3388484(+) (pilE) [Melaminivora jejuensis strain KACC 19629]
MQRTMRSVQKGFTLIELMIVVAIIGILAAVALPAYQDYTARAKISEIIIAASSPKSLISEAFQADSLAGVAAAATEYNAR
AQTEKQSKIVDNIQIDGANGVITVTSVTGGGLPSDAQTTTLTWTPNVQGALLAAGVSGAIDWACASTTNTTATARGLVVG
TPGTMPAKYAPSECR

Nucleotide


Download         Length: 528 bp        

>NTDB_id=638416 LVC68_RS15685 WP_269783622.1 3387957..3388484(+) (pilE) [Melaminivora jejuensis strain KACC 19629]
ATGCAACGCACCATGCGCTCCGTACAAAAGGGCTTCACCCTGATCGAACTGATGATCGTCGTGGCGATCATCGGCATCCT
GGCCGCCGTGGCCCTGCCCGCCTACCAGGACTACACCGCCCGCGCCAAGATCTCTGAAATCATCATCGCCGCCAGCTCGC
CCAAGAGCCTGATCAGCGAGGCCTTCCAAGCCGACAGTCTGGCCGGCGTGGCCGCTGCTGCGACCGAGTACAACGCCCGC
GCTCAGACTGAGAAGCAGTCGAAGATTGTGGACAACATCCAGATCGACGGCGCCAACGGCGTGATCACCGTGACCAGCGT
GACTGGCGGCGGCTTGCCCAGCGATGCCCAGACCACAACGCTGACCTGGACGCCCAACGTGCAGGGCGCTCTCCTGGCTG
CCGGGGTTTCGGGCGCCATCGACTGGGCTTGCGCCTCGACCACCAACACCACCGCCACTGCACGCGGCCTGGTAGTGGGT
ACACCGGGCACCATGCCCGCCAAGTACGCACCTTCCGAGTGCCGCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)

Pilin (bacterial filament) (Pilin)

(40-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

48.691

100

0.531
  pilA Ralstonia pseudosolanacearum GMI1000

41.848

100

0.44
  pilA2 Legionella pneumophila strain ERS1305867

43.195

96.571

0.417
  pilA2 Legionella pneumophila str. Paris

43.195

96.571

0.417
  comP Acinetobacter baylyi ADP1

40.588

97.143

0.394
  pilE Neisseria gonorrhoeae MS11

36.957

100

0.389
  pilE Neisseria gonorrhoeae strain FA1090

37.64

100

0.383
  pilA/pilA1 Eikenella corrodens VA1

41.935

88.571

0.371