Detailed information NTG0638416

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: LVC68_RS15685
• Genome accession: NZ_CP089742
• Coordinates: 3387957..3388484 (+)
• Length: 175 a.a.
• NCBI ID: WP_269783622.1
• Uniprot ID: -
• Organism: Melaminivora jejuensis strain KACC 19629
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3382957..3393484

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LVC68_RS15650 (LVC68_15650)	sucD	3383289..3384182 (+)	894	WP_209423588.1	succinate--CoA ligase subunit alpha	-
LVC68_RS15655 (LVC68_15655)	-	3384468..3385241 (+)	774	WP_209423587.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
LVC68_RS15660 (LVC68_15660)	-	3385337..3385945 (+)	609	WP_209424164.1	FHA domain-containing protein	-
LVC68_RS15665 (LVC68_15665)	-	3386026..3386109 (+)	84	WP_232654404.1	hypothetical protein	-
LVC68_RS15670 (LVC68_15670)	-	3386156..3387412 (+)	1257	WP_209423586.1	RNA-guided endonuclease TnpB family protein	-
LVC68_RS15675 (LVC68_15675)	-	3387490..3387999 (+)	510	WP_232653791.1	hypothetical protein	-
LVC68_RS15685 (LVC68_15685)	pilE	3387957..3388484 (+)	528	WP_269783622.1	pilin	Machinery gene
LVC68_RS15690 (LVC68_15690)	-	3388606..3389379 (+)	774	WP_209423585.1	hypothetical protein	-
LVC68_RS15700 (LVC68_15700)	pilE	3389513..3390031 (+)	519	WP_209423584.1	pilin	Machinery gene
LVC68_RS15705 (LVC68_15705)	-	3390467..3390922 (+)	456	WP_209423583.1	DUF4351 domain-containing protein	-
LVC68_RS15710 (LVC68_15710)	-	3391014..3391472 (+)	459	WP_209423582.1	carboxymuconolactone decarboxylase family protein	-
LVC68_RS15715 (LVC68_15715)	-	3391484..3392083 (-)	600	WP_232653793.1	Uma2 family endonuclease	-
LVC68_RS15725 (LVC68_15725)	-	3392339..3392755 (-)	417	WP_209423580.1	type II toxin-antitoxin system HicB family antitoxin	-
LVC68_RS15730 (LVC68_15730)	-	3392764..3392949 (-)	186	WP_209423579.1	type II toxin-antitoxin system HicA family toxin	-

Sequence

Protein

• Length: 175 a.a.
• Molecular weight: 17959.53 Da
• Isoelectric Point: 6.2693

>NTDB_id=638416 LVC68_RS15685 WP_269783622.1 3387957..3388484(+) (pilE) [Melaminivora jejuensis strain KACC 19629]
MQRTMRSVQKGFTLIELMIVVAIIGILAAVALPAYQDYTARAKISEIIIAASSPKSLISEAFQADSLAGVAAAATEYNAR
AQTEKQSKIVDNIQIDGANGVITVTSVTGGGLPSDAQTTTLTWTPNVQGALLAAGVSGAIDWACASTTNTTATARGLVVG
TPGTMPAKYAPSECR

Nucleotide

• Length: 528 bp

>NTDB_id=638416 LVC68_RS15685 WP_269783622.1 3387957..3388484(+) (pilE) [Melaminivora jejuensis strain KACC 19629]
ATGCAACGCACCATGCGCTCCGTACAAAAGGGCTTCACCCTGATCGAACTGATGATCGTCGTGGCGATCATCGGCATCCT
GGCCGCCGTGGCCCTGCCCGCCTACCAGGACTACACCGCCCGCGCCAAGATCTCTGAAATCATCATCGCCGCCAGCTCGC
CCAAGAGCCTGATCAGCGAGGCCTTCCAAGCCGACAGTCTGGCCGGCGTGGCCGCTGCTGCGACCGAGTACAACGCCCGC
GCTCAGACTGAGAAGCAGTCGAAGATTGTGGACAACATCCAGATCGACGGCGCCAACGGCGTGATCACCGTGACCAGCGT
GACTGGCGGCGGCTTGCCCAGCGATGCCCAGACCACAACGCTGACCTGGACGCCCAACGTGCAGGGCGCTCTCCTGGCTG
CCGGGGTTTCGGGCGCCATCGACTGGGCTTGCGCCTCGACCACCAACACCACCGCCACTGCACGCGGCCTGGTAGTGGGT
ACACCGGGCACCATGCCCGCCAAGTACGCACCTTCCGAGTGCCGCTGA

Domains

Predicted by InterProScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (7-32)

• Pilin (bacterial filament) (Pilin) (40-175)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	48.691	100	0.531
pilA	Ralstonia pseudosolanacearum GMI1000	41.848	100	0.44
pilA2	Legionella pneumophila strain ERS1305867	43.195	96.571	0.417
pilA2	Legionella pneumophila str. Paris	43.195	96.571	0.417
comP	Acinetobacter baylyi ADP1	40.588	97.143	0.394
pilE	Neisseria gonorrhoeae MS11	36.957	100	0.389
pilE	Neisseria gonorrhoeae strain FA1090	37.64	100	0.383
pilA/pilA1	Eikenella corrodens VA1	41.935	88.571	0.371