Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LQF09_RS14610 Genome accession   NZ_CP088935
Coordinates   3187210..3188928 (+) Length   572 a.a.
NCBI ID   WP_002942984.1    Uniprot ID   A0A351A623
Organism   Thauera sp. WB-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3182210..3193928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQF09_RS14590 (LQF09_14595) - 3182714..3183499 (-) 786 WP_002924661.1 EI24 domain-containing protein -
  LQF09_RS14595 (LQF09_14600) ffh 3183511..3184881 (-) 1371 WP_002924663.1 signal recognition particle protein -
  LQF09_RS14600 (LQF09_14605) ccsA 3184930..3185763 (+) 834 WP_002924665.1 cytochrome c biogenesis protein CcsA -
  LQF09_RS14605 (LQF09_14610) - 3185841..3187133 (+) 1293 WP_270068299.1 CNNM domain-containing protein -
  LQF09_RS14610 (LQF09_14615) pilB 3187210..3188928 (+) 1719 WP_002942984.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LQF09_RS14615 (LQF09_14620) pilC 3188948..3190180 (+) 1233 WP_002942986.1 type II secretion system F family protein Machinery gene
  LQF09_RS14620 (LQF09_14625) pilD 3190181..3191032 (+) 852 WP_002942988.1 A24 family peptidase Machinery gene
  LQF09_RS14625 (LQF09_14630) - 3191162..3191425 (+) 264 WP_002942990.1 zinc ribbon domain-containing protein -
  LQF09_RS14630 (LQF09_14635) - 3191466..3192092 (+) 627 WP_270068300.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62376.66 Da        Isoelectric Point: 5.5843

>NTDB_id=634078 LQF09_RS14610 WP_002942984.1 3187210..3188928(+) (pilB) [Thauera sp. WB-2]
MAAATKPTLSGFARALIQHGHLQEADAQACSSHANEGSNAFILEVASRGLMKCADMARFAAETFGYPLLDIAAVDASSYA
RDAVDRKLIQKHQIAPLAKRQNRLTLATADPSNMRALDEIRFQTGMQLDLVIAEADKLKRVIDALVESAADTLRDLTGEA
FDMDLLQQDTPAQQQRDEDEASEVDDAPVVKFIQKVLIDAINEGASDIHFEPYEKFYRIRVRTDGILRDIAQPPLVLKEK
IAARIKVISRLDISERRVPQDGRMKLVLSKNKAIDFRVSTLPTLHGEKIVMRILDPSSAMLGIDALGYEPDQRKALLAAV
ERPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPAEINLPGINQVNVNEKAGLTFSVALRAFLRQDPDIIMVG
EIRDLETAEISIKAAQTGHLVMSTLHTNDAPTTLERLKNMGVAPFNIASSVILITAQRLARRLCSCKKAADIPFEALIEA
GFSAEELDGSWQPMGPVGCDRCKGSGYKGRLGIYQVMPISEEIAHIIMTGGNSMDIAAQAEREGVRDLRKSGLLKVKQGI
TSLEEVLATTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=634078 LQF09_RS14610 WP_002942984.1 3187210..3188928(+) (pilB) [Thauera sp. WB-2]
ATGGCGGCAGCGACAAAACCGACCCTGAGCGGGTTCGCCAGGGCACTGATACAGCACGGACATCTGCAGGAAGCAGATGC
GCAGGCCTGTTCCTCCCATGCCAACGAGGGCAGCAACGCATTCATCCTCGAGGTCGCCAGCCGCGGCCTGATGAAGTGCG
CCGACATGGCGCGTTTTGCGGCCGAGACCTTCGGTTACCCCCTGCTCGACATCGCAGCGGTCGACGCCTCCTCGTACGCA
CGTGACGCAGTCGATCGCAAGCTGATACAGAAGCACCAGATCGCACCTCTGGCCAAACGCCAGAATCGCCTCACGCTGGC
TACCGCCGATCCGTCGAACATGCGGGCGCTTGACGAGATCCGCTTCCAGACCGGAATGCAGCTCGACCTCGTGATCGCCG
AGGCCGACAAGCTCAAGCGCGTCATCGATGCGCTGGTCGAATCCGCGGCGGACACGCTGCGCGACCTCACGGGTGAAGCC
TTCGACATGGACCTGCTGCAGCAGGACACGCCTGCGCAGCAGCAGCGCGACGAGGACGAGGCCTCAGAGGTCGATGACGC
CCCCGTCGTCAAGTTCATCCAGAAGGTCCTGATCGACGCAATCAACGAGGGGGCCTCCGACATCCACTTCGAGCCCTACG
AGAAGTTCTATCGCATCCGCGTACGCACCGATGGCATCCTGCGCGACATCGCCCAGCCGCCGTTGGTACTCAAGGAGAAG
ATCGCTGCCCGCATCAAGGTGATCTCGCGCCTCGACATCTCCGAGCGACGCGTCCCTCAGGACGGCCGCATGAAGCTGGT
TTTGTCAAAGAACAAGGCCATCGACTTTCGCGTATCGACCCTGCCGACACTGCATGGCGAGAAGATCGTCATGCGCATCC
TCGACCCGAGCTCGGCGATGCTCGGCATCGATGCGCTCGGCTACGAGCCCGACCAGCGCAAGGCGCTGCTCGCAGCGGTC
GAGCGCCCCTACGGCATGATCCTCGTCACCGGCCCGACGGGCTCGGGCAAGACCGTGTCGCTCTACACCTGCCTCAACCT
GCTCAACAAGCCGGGCGTGAATATCTCCACGGCAGAGGACCCGGCCGAAATCAACCTGCCCGGCATCAACCAGGTCAACG
TCAACGAGAAGGCGGGGCTCACCTTTTCGGTCGCACTCAGGGCCTTCCTGCGCCAGGATCCCGATATCATCATGGTCGGT
GAAATTCGCGACCTCGAGACCGCGGAGATCTCGATCAAGGCGGCGCAGACCGGCCACCTGGTGATGTCGACCCTGCACAC
GAACGACGCCCCCACCACGCTCGAGCGACTCAAGAACATGGGCGTGGCGCCGTTCAACATTGCATCGTCGGTGATCCTCA
TCACGGCACAGCGCCTCGCGCGCCGGCTATGCTCGTGCAAGAAGGCCGCGGACATTCCCTTCGAGGCGCTCATCGAGGCG
GGATTCAGCGCCGAGGAACTGGACGGCAGCTGGCAACCCATGGGGCCGGTCGGTTGCGACCGCTGCAAGGGCAGTGGCTA
CAAGGGGCGCCTGGGCATCTACCAGGTCATGCCGATCAGCGAGGAGATCGCCCACATCATCATGACCGGCGGCAACTCGA
TGGACATCGCGGCGCAGGCGGAACGCGAAGGCGTGCGCGACCTGCGCAAGTCCGGGCTTCTCAAGGTCAAGCAGGGTATA
ACCTCGCTCGAAGAAGTCCTGGCGACGACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-148)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A351A623

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.076

100

0.565
  pilB Acinetobacter baylyi ADP1

54.704

100

0.549
  pilB Legionella pneumophila strain ERS1305867

52.632

99.65

0.524
  pilF Neisseria gonorrhoeae MS11

51.839

99.825

0.517
  pilB Vibrio cholerae strain A1552

51.758

89.51

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

49.146

92.133

0.453
  pilB Vibrio campbellii strain DS40M4

49.049

91.958

0.451
  pilF Thermus thermophilus HB27

38.246

99.65

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.385

90.909

0.367
  pilB/pilB1 Synechocystis sp. PCC 6803

40.504

90.21

0.365