Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   N5P33_RS25445 Genome accession   NZ_CP104720
Coordinates   5485457..5486080 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain NY4593     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5480457..5491080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5P33_RS25425 (N5P33_25425) aroB 5481052..5482158 (-) 1107 WP_028681386.1 3-dehydroquinate synthase -
  N5P33_RS25430 (N5P33_25430) aroK 5482208..5482726 (-) 519 WP_003095833.1 shikimate kinase AroK -
  N5P33_RS25435 (N5P33_25435) pilQ 5482738..5484882 (-) 2145 WP_261734948.1 type 4a pilus secretin PilQ Machinery gene
  N5P33_RS25440 (N5P33_25440) comL/pilP 5484936..5485460 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  N5P33_RS25445 (N5P33_25445) pilO 5485457..5486080 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  N5P33_RS25450 (N5P33_25450) pilN 5486077..5486673 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  N5P33_RS25455 (N5P33_25455) pilM 5486673..5487737 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  N5P33_RS25460 (N5P33_25460) - 5487922..5490393 (+) 2472 WP_023089702.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=632498 N5P33_RS25445 WP_003103268.1 5485457..5486080(-) (pilO) [Pseudomonas aeruginosa strain NY4593]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=632498 N5P33_RS25445 WP_003103268.1 5485457..5486080(-) (pilO) [Pseudomonas aeruginosa strain NY4593]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401