Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N5855_RS22630 Genome accession   NZ_CP104629
Coordinates   4733152..4734537 (-) Length   461 a.a.
NCBI ID   WP_040234494.1    Uniprot ID   -
Organism   Escherichia coli strain AHM21C2872I     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4728152..4739537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5855_RS22595 (N5855_22545) mutT 4728483..4728872 (+) 390 WP_000736013.1 8-oxo-dGTP diphosphatase MutT -
  N5855_RS22600 (N5855_22550) yacG 4729087..4729284 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  N5855_RS22605 (N5855_22555) zapD 4729294..4730037 (-) 744 WP_001194734.1 cell division protein ZapD -
  N5855_RS22610 (N5855_22560) coaE 4730037..4730657 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  N5855_RS22615 (N5855_22565) - 4730682..4730726 (+) 45 WP_120795372.1 protein YacM -
  N5855_RS22620 (N5855_22570) guaC 4730882..4731925 (+) 1044 WP_001217338.1 GMP reductase -
  N5855_RS22625 (N5855_22575) hofC 4731960..4733162 (-) 1203 WP_000157234.1 protein transport protein HofC -
  N5855_RS22630 (N5855_22580) pilB 4733152..4734537 (-) 1386 WP_040234494.1 type II secretion system protein GspE Machinery gene
  N5855_RS22635 (N5855_22585) pilA 4734547..4734987 (-) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  N5855_RS22640 (N5855_22590) nadC 4735190..4736083 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5855_RS22645 (N5855_22595) ampD 4736171..4736722 (+) 552 WP_000923720.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5855_RS22650 (N5855_22600) ampE 4736719..4737573 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  N5855_RS22655 (N5855_22605) aroP 4737616..4738989 (-) 1374 WP_000969912.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50579.38 Da        Isoelectric Point: 6.7135

>NTDB_id=631933 N5855_RS22630 WP_040234494.1 4733152..4734537(-) (pilB) [Escherichia coli strain AHM21C2872I]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGKAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=631933 N5855_RS22630 WP_040234494.1 4733152..4734537(-) (pilB) [Escherichia coli strain AHM21C2872I]
ATGAATATTCCACAGCTCACGGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAAAGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CGCAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATTCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATTATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGCCAGCAAGGGGAGCCCATCCATATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGTGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.598

100

0.412
  pilB Vibrio campbellii strain DS40M4

48.32

83.948

0.406
  pilB Vibrio cholerae strain A1552

46.465

85.9

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.632

83.731

0.39
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

44.162

85.466

0.377
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.831

88.72

0.362