Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5855_RS22635 Genome accession   NZ_CP104629
Coordinates   4734547..4734987 (-) Length   146 a.a.
NCBI ID   WP_000360901.1    Uniprot ID   -
Organism   Escherichia coli strain AHM21C2872I     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4729547..4739987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5855_RS22610 (N5855_22560) coaE 4730037..4730657 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  N5855_RS22615 (N5855_22565) - 4730682..4730726 (+) 45 WP_120795372.1 protein YacM -
  N5855_RS22620 (N5855_22570) guaC 4730882..4731925 (+) 1044 WP_001217338.1 GMP reductase -
  N5855_RS22625 (N5855_22575) hofC 4731960..4733162 (-) 1203 WP_000157234.1 protein transport protein HofC -
  N5855_RS22630 (N5855_22580) pilB 4733152..4734537 (-) 1386 WP_040234494.1 type II secretion system protein GspE Machinery gene
  N5855_RS22635 (N5855_22585) pilA 4734547..4734987 (-) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  N5855_RS22640 (N5855_22590) nadC 4735190..4736083 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5855_RS22645 (N5855_22595) ampD 4736171..4736722 (+) 552 WP_000923720.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5855_RS22650 (N5855_22600) ampE 4736719..4737573 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  N5855_RS22655 (N5855_22605) aroP 4737616..4738989 (-) 1374 WP_000969912.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15648.78 Da        Isoelectric Point: 4.3938

>NTDB_id=631934 N5855_RS22635 WP_000360901.1 4734547..4734987(-) (pilA) [Escherichia coli strain AHM21C2872I]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQNDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=631934 N5855_RS22635 WP_000360901.1 4734547..4734987(-) (pilA) [Escherichia coli strain AHM21C2872I]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAATGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363