Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LP089_RS04710 Genome accession   NZ_CP087863
Coordinates   969154..970332 (+) Length   392 a.a.
NCBI ID   WP_115370245.1    Uniprot ID   -
Organism   Moraxella bovis strain SAM57926     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 964154..975332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LP089_RS04685 (LP089_04660) - 964897..965280 (+) 384 WP_264676962.1 hypothetical protein -
  LP089_RS04690 (LP089_04665) nuoC 965727..967553 (+) 1827 WP_264678400.1 NADH-quinone oxidoreductase subunit C/D -
  LP089_RS04695 (LP089_04670) - 968195..968428 (+) 234 WP_264676961.1 hypothetical protein -
  LP089_RS04700 (LP089_04675) - 968421..968570 (+) 150 WP_264676960.1 hypothetical protein -
  LP089_RS04705 (LP089_04680) - 968574..968819 (+) 246 WP_264676959.1 hypothetical protein -
  LP089_RS04710 (LP089_04685) pilU 969154..970332 (+) 1179 WP_115370245.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LP089_RS04715 (LP089_04690) - 970346..971092 (+) 747 WP_264676958.1 hypothetical protein -
  LP089_RS04720 (LP089_04695) - 971114..971398 (+) 285 WP_115370144.1 hypothetical protein -
  LP089_RS04725 (LP089_04700) - 971398..971811 (+) 414 WP_115370143.1 type II toxin-antitoxin system VapC family toxin -
  LP089_RS04730 (LP089_04705) nuoE 971940..972449 (+) 510 WP_078273087.1 NADH-quinone oxidoreductase subunit NuoE -
  LP089_RS04735 (LP089_04710) nuoF 972446..973864 (+) 1419 WP_264676957.1 NADH-quinone oxidoreductase subunit NuoF -

Sequence

Protein


Download         Length: 392 a.a.        Molecular weight: 44361.78 Da        Isoelectric Point: 6.3251

>NTDB_id=631928 LP089_RS04710 WP_115370245.1 969154..970332(+) (pilU) [Moraxella bovis strain SAM57926]
MRFAEETLNEAKGMMYNMLTKVVEIGASDLFITAEFPPSVKHQGLMKPLGKQVLSADKTKLFAYSIMNDYQRDEFEREME
CNFAINVPNVSRFRVNVFIQQQQVGMVIRTIAAEIPNFDKLRLPPQLRDVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRN
ENSAGHIITVEDPVEYVHKHKKSMITHREVGVDTHSWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTL
HANNANQTLDRIINFFPEERRQQLLMDLSSNMKAIISQRLIRTEDGKGRRAAVEIMLNTRLVADLILKGEMHELKAIMTK
SREVGMQTFDQALFDLYDEGAISYDEAIRNADSPNELRLQIKLKGTRRDGQNEVASSLSLHADVEEADDKKE

Nucleotide


Download         Length: 1179 bp        

>NTDB_id=631928 LP089_RS04710 WP_115370245.1 969154..970332(+) (pilU) [Moraxella bovis strain SAM57926]
ATGCGATTTGCCGAAGAAACCCTAAACGAAGCCAAAGGTATGATGTATAATATGCTCACCAAAGTTGTTGAGATTGGGGC
ATCGGATTTATTTATCACGGCAGAATTTCCGCCGAGCGTCAAACATCAAGGCTTGATGAAACCGCTTGGCAAGCAGGTAT
TAAGTGCCGACAAAACCAAACTGTTTGCGTACAGTATCATGAATGACTATCAGCGTGATGAATTTGAACGTGAAATGGAA
TGTAACTTTGCCATTAACGTGCCAAATGTGAGTCGTTTTCGTGTGAATGTCTTTATCCAACAGCAACAGGTTGGTATGGT
GATTCGTACGATTGCGGCTGAGATTCCAAACTTTGACAAATTAAGATTGCCGCCCCAACTGCGTGATGTCATCATGGAAA
AGCGTGGTTTGGTGCTGGTCGTGGGCGGTACGGGGTCTGGTAAGTCCACGAGCCTTGCGGCAATGATTGATTATCGTAAC
GAAAACAGTGCAGGGCATATCATCACGGTGGAAGACCCTGTGGAATATGTGCATAAGCACAAAAAATCAATGATTACGCA
CCGTGAAGTGGGCGTAGACACGCATTCTTGGCACCACGCTCTAAAAAACACGCTAAGGCAAGCCCCTGATGTGATTTTGA
TTGGCGAGATACGAGATACCGAGACCATGGAGCACGCCATTGCCTTTGCTGAGACGGGGCATTTGTGCTTGGGCACGCTC
CACGCCAACAACGCCAACCAAACGCTTGACCGTATCATCAACTTTTTCCCAGAAGAACGCCGTCAGCAGTTATTGATGGA
TTTGTCCAGCAATATGAAAGCGATTATTTCTCAACGCCTAATCCGTACCGAAGACGGCAAGGGGCGGCGGGCGGCGGTGG
AGATCATGCTTAACACCCGCCTTGTGGCAGATTTGATTTTAAAGGGTGAAATGCACGAGTTAAAAGCGATTATGACAAAA
AGCCGTGAAGTGGGTATGCAGACCTTTGACCAAGCGTTGTTTGACCTATATGATGAAGGGGCTATCTCATATGATGAAGC
GATTCGTAATGCCGACTCGCCTAATGAGCTACGCTTACAAATCAAACTCAAAGGTACTCGCCGTGATGGACAAAATGAAG
TGGCAAGCTCATTATCACTTCACGCTGATGTCGAAGAGGCTGATGATAAAAAGGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(54-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.258

94.898

0.543
  pilU Acinetobacter baylyi ADP1

57.471

88.776

0.51
  pilU Vibrio cholerae strain A1552

54.142

86.224

0.467
  pilT Acinetobacter baumannii strain A118

43.027

85.969

0.37
  pilT Acinetobacter baylyi ADP1

43.027

85.969

0.37
  pilT Acinetobacter nosocomialis M2

43.027

85.969

0.37
  pilT Acinetobacter baumannii D1279779

43.027

85.969

0.37
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.522

86.99

0.37