Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LPS07_RS17125 Genome accession   NZ_CP087716
Coordinates   3556396..3558108 (+) Length   570 a.a.
NCBI ID   WP_230445083.1    Uniprot ID   -
Organism   Acinetobacter pittii strain AP2044     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3551396..3563108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPS07_RS17090 (LPS07_17090) nusA 3552285..3553769 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  LPS07_RS17095 (LPS07_17095) rimP 3553807..3554331 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  LPS07_RS17115 (LPS07_17115) secG 3554969..3555298 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  LPS07_RS17120 (LPS07_17120) tpiA 3555311..3556105 (-) 795 WP_032063691.1 triose-phosphate isomerase -
  LPS07_RS17125 (LPS07_17125) pilB 3556396..3558108 (+) 1713 WP_230445083.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LPS07_RS17130 (LPS07_17130) pilC 3558135..3559361 (+) 1227 WP_002114869.1 type II secretion system F family protein Machinery gene
  LPS07_RS17135 (LPS07_17135) pilD 3559361..3560221 (+) 861 WP_032070754.1 prepilin peptidase Machinery gene
  LPS07_RS17140 (LPS07_17140) coaE 3560223..3560819 (+) 597 WP_005065869.1 dephospho-CoA kinase -
  LPS07_RS17145 (LPS07_17145) - 3560816..3561730 (-) 915 WP_032063689.1 DMT family transporter -
  LPS07_RS17150 (LPS07_17150) rlmB 3561766..3562515 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LPS07_RS17155 (LPS07_17155) - 3562618..3562944 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63515.87 Da        Isoelectric Point: 5.0271

>NTDB_id=631223 LPS07_RS17125 WP_230445083.1 3556396..3558108(+) (pilB) [Acinetobacter pittii strain AP2044]
MSVLNTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TDQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=631223 LPS07_RS17125 WP_230445083.1 3556396..3558108(+) (pilB) [Acinetobacter pittii strain AP2044]
ATGTCAGTCTTAAATACATCTCCAAAATTTTCGGGGTTTTTTCGGCGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCAGAAACAATTTCTATTGAATTTGCGGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTGGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCAACCAGCAACCCGAGTAATATTGAAGCGATTGATGCCATTCGCTTTAATAGTAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCGCAGCAATTTACCGAAGAAAGTAGCTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCCCCCTCACAAGAGGATGAGGAAGAAACGCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCAATTCGTATGGGGGCTTCAGACTTACATTTTGAACCATACGAGAAATCAT
ATCGGGTCCGTTACCGAGTCGATGGCGTGTTGCGTCAGATTGCAAATCCACCTTTGCAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGAGTGCCTCAAGACGGTCGTATCAAGCTTAAGTTATCAAA
AACTAAAGCAATTGATTTTCGTGTGAACTCACTTCCAACCCTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCTT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGTTCGAATATTTCTACCGCCGAAGATCCAGTTGAAATTAACCTTGAAGGAATTAATCAGGTTAACGTCAACCCAA
AAGTAGGTCTGACTTTTTCTGCTGCACTTAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTAATGTCCACACTGCATACCAATAGTGC
GCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGACCAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTAGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTACGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTAACTTCC
TTACAAGAAGTTAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.912

100

0.949
  pilB Acinetobacter baylyi ADP1

78.07

100

0.781
  pilB Legionella pneumophila strain ERS1305867

50.783

100

0.512
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.321

92.982

0.477
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

36.655

98.596

0.361