Detailed information NTG0630381

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: LO769_RS10995
• Genome accession: NZ_CP087600
• Coordinates: 2160173..2161246 (-)
• Length: 357 a.a.
• NCBI ID: WP_014570824.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain IBB109
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2155173..2166246

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LO769_RS10950 (LO769_10945)	-	2155287..2156096 (-)	810	WP_014570791.1	metal ABC transporter permease	-
LO769_RS10955 (LO769_10950)	-	2156089..2156826 (-)	738	WP_058221575.1	metal ABC transporter ATP-binding protein	-
LO769_RS10960 (LO769_10955)	-	2157003..2157845 (-)	843	WP_003129990.1	metal ABC transporter substrate-binding protein	-
LO769_RS10965 (LO769_10960)	-	2157842..2158279 (-)	438	WP_003129992.1	zinc-dependent MarR family transcriptional regulator	-
LO769_RS10970 (LO769_10965)	comGG	2158385..2158669 (-)	285	WP_003129993.1	competence type IV pilus minor pilin ComGG	Machinery gene
LO769_RS10975 (LO769_10970)	comGF	2158708..2159154 (-)	447	WP_029344525.1	competence type IV pilus minor pilin ComGF	Machinery gene
LO769_RS10980 (LO769_10975)	comGE	2159117..2159413 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
LO769_RS10985 (LO769_10980)	comGD	2159385..2159816 (-)	432	WP_080585155.1	competence type IV pilus minor pilin ComGD	Machinery gene
LO769_RS10990 (LO769_10985)	comGC	2159776..2160159 (-)	384	WP_081040865.1	competence type IV pilus major pilin ComGC	Machinery gene
LO769_RS10995 (LO769_10990)	comGB	2160173..2161246 (-)	1074	WP_014570824.1	competence type IV pilus assembly protein ComGB	Machinery gene
LO769_RS11000 (LO769_10995)	comGA	2161140..2162079 (-)	940	Protein_2136	competence type IV pilus ATPase ComGA	-

Sequence

Protein

• Length: 357 a.a.
• Molecular weight: 40366.59 Da
• Isoelectric Point: 10.4062

>NTDB_id=630381 LO769_RS10995 WP_014570824.1 2160173..2161246(-) (comGB) [Lactococcus lactis subsp. lactis strain IBB109]
MSLGHQKIGIKKLISYLQMDISHLLKPRGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKKFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide

• Length: 1074 bp

>NTDB_id=630381 LO769_RS10995 WP_014570824.1 2160173..2161246(-) (comGB) [Lactococcus lactis subsp. lactis strain IBB109]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAGAGGGAAAAAATTAAGGCTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAAAATTTACATTGAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTGGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACCTTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACATTTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (41-159)

• Type II secretion system (T2SS), protein F (T2SSF) (227-348)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	82.698	95.518	0.79
comYB	Streptococcus gordonii str. Challis substr. CH1	53.666	95.518	0.513
comYB	Streptococcus mutans UA140	53.372	95.518	0.51
comYB	Streptococcus mutans UA159	53.079	95.518	0.507
comGB/cglB	Streptococcus pneumoniae TIGR4	51.343	93.838	0.482
comGB/cglB	Streptococcus pneumoniae D39	51.343	93.838	0.482
comGB/cglB	Streptococcus pneumoniae R6	51.343	93.838	0.482
comGB/cglB	Streptococcus pneumoniae Rx1	51.343	93.838	0.482
comGB/cglB	Streptococcus mitis SK321	51.045	93.838	0.479
comGB/cglB	Streptococcus mitis NCTC 12261	50.149	93.838	0.471