Detailed information NTG0630377

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LO769_RS10975
• Genome accession: NZ_CP087600
• Coordinates: 2158708..2159154 (-)
• Length: 148 a.a.
• NCBI ID: WP_029344525.1
• Uniprot ID: A0AAP4JUI5
• Organism: Lactococcus lactis subsp. lactis strain IBB109
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2153708..2164154

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LO769_RS10940 (LO769_10935)	-	2153801..2154178 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
LO769_RS10945 (LO769_10940)	-	2154382..2155248 (+)	867	WP_153927515.1	RluA family pseudouridine synthase	-
LO769_RS10950 (LO769_10945)	-	2155287..2156096 (-)	810	WP_014570791.1	metal ABC transporter permease	-
LO769_RS10955 (LO769_10950)	-	2156089..2156826 (-)	738	WP_058221575.1	metal ABC transporter ATP-binding protein	-
LO769_RS10960 (LO769_10955)	-	2157003..2157845 (-)	843	WP_003129990.1	metal ABC transporter substrate-binding protein	-
LO769_RS10965 (LO769_10960)	-	2157842..2158279 (-)	438	WP_003129992.1	zinc-dependent MarR family transcriptional regulator	-
LO769_RS10970 (LO769_10965)	comGG	2158385..2158669 (-)	285	WP_003129993.1	competence type IV pilus minor pilin ComGG	Machinery gene
LO769_RS10975 (LO769_10970)	comGF	2158708..2159154 (-)	447	WP_029344525.1	competence type IV pilus minor pilin ComGF	Machinery gene
LO769_RS10980 (LO769_10975)	comGE	2159117..2159413 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
LO769_RS10985 (LO769_10980)	comGD	2159385..2159816 (-)	432	WP_080585155.1	competence type IV pilus minor pilin ComGD	Machinery gene
LO769_RS10990 (LO769_10985)	comGC	2159776..2160159 (-)	384	WP_081040865.1	competence type IV pilus major pilin ComGC	Machinery gene
LO769_RS10995 (LO769_10990)	comGB	2160173..2161246 (-)	1074	WP_014570824.1	competence type IV pilus assembly protein ComGB	Machinery gene
LO769_RS11000 (LO769_10995)	comGA	2161140..2162079 (-)	940	Protein_2136	competence type IV pilus ATPase ComGA	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=630377 LO769_RS10975 WP_029344525.1 2158708..2159154(-) (comGF) [Lactococcus lactis subsp. lactis strain IBB109]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=630377 LO769_RS10975 WP_029344525.1 2158708..2159154(-) (comGF) [Lactococcus lactis subsp. lactis strain IBB109]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	93.919	0.716
comYF	Streptococcus mutans UA140	48.227	95.27	0.459
comYF	Streptococcus mutans UA159	47.518	95.27	0.453
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385