Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N5O79_RS11205 Genome accession   NZ_CP104516
Coordinates   2193493..2194854 (+) Length   453 a.a.
NCBI ID   WP_261307162.1    Uniprot ID   -
Organism   Escherichia coli strain E4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2188493..2199854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O79_RS11180 (N5O79_11180) aroP 2189017..2190390 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  N5O79_RS11185 (N5O79_11185) ampE 2190433..2191287 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  N5O79_RS11190 (N5O79_11190) ampD 2191284..2191835 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5O79_RS11195 (N5O79_11195) nadC 2191923..2192816 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5O79_RS11200 (N5O79_11200) pilA 2193019..2193459 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  N5O79_RS11205 (N5O79_11205) pilB 2193493..2194854 (+) 1362 WP_261307162.1 type II secretion system protein GspE Machinery gene
  N5O79_RS11210 (N5O79_11210) hofC 2194844..2196046 (+) 1203 WP_000157266.1 protein transport protein HofC -
  N5O79_RS11215 (N5O79_11215) guaC 2196081..2197124 (-) 1044 WP_001217335.1 GMP reductase -
  N5O79_RS11220 (N5O79_11220) - 2197280..2197324 (-) 45 WP_120795372.1 protein YacM -
  N5O79_RS11225 (N5O79_11225) coaE 2197349..2197969 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  N5O79_RS11230 (N5O79_11230) zapD 2197969..2198712 (+) 744 WP_001194734.1 cell division protein ZapD -
  N5O79_RS11235 (N5O79_11235) yacG 2198722..2198919 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  N5O79_RS11240 (N5O79_11240) mutT 2199135..2199524 (-) 390 WP_000736007.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49764.32 Da        Isoelectric Point: 6.6612

>NTDB_id=630165 N5O79_RS11205 WP_261307162.1 2193493..2194854(+) (pilB) [Escherichia coli strain E4]
MCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRT
LQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVELAGNAVSFR
IATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLNTADINICS
VEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRL
QQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFEVLPITPVI
RQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=630165 N5O79_RS11205 WP_261307162.1 2193493..2194854(+) (pilB) [Escherichia coli strain E4]
CTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGTTGCGGTAGTCGATGCACCTTCGCA
TGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCTGGACGCGCCAACAAATGGAAGGTC
ACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCCAAAGCAGAGTTGCTAACTCGAACG
TTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAATGCCTACCGCATCCGCTTGCGTAT
CGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAACCGCCAGATTAAAAGTGCTGGGAA
ACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTGGCAGGAAACGCCGTCTCATTTCGT
ATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCAGGTGGGTCAGGCACTGGATGTCAA
CACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAACCACAGGGACTGGTGCTGGTAACTG
GCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAATACCGCTGACATTAATATTTGTAGC
GTCGAAGATCCAGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCCGCGTGCCGGACTCACCTTTCAGGG
CGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCCGCGATGGCGAAACAGCAGAGATCG
CTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCCACCTGCGAAACGCTGGTACGTTTA
CAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGCCCAGCGTCTGGTACGCAAACTTTG
CCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGCCATCGCCGCTGCCCCACTGGCAGG
CACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAAGTTCTGCCCATAACGCCGGTCATT
CGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACAGGCGGGTATGCGTACGCTTTTTGA
AAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCGTACTGGGGATGCCGCATGGCGAGT
AA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(1-60)

Type II/IV secretion system protein (T2SSE)

(80-345)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

84.768

0.424
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.419
  pilB Acinetobacter baylyi ADP1

39.957

100

0.413
  pilB Vibrio campbellii strain DS40M4

48.187

85.21

0.411
  pilB Vibrio cholerae strain A1552

46.667

86.093

0.402
  pilB Vibrio parahaemolyticus RIMD 2210633

46.154

86.093

0.397
  pilB Haemophilus influenzae 86-028NP

45.22

85.43

0.386
  pilB Acinetobacter baumannii D1279779

43.655

86.976

0.38
  pilB Haemophilus influenzae Rd KW20

44.444

85.43

0.38
  pilF Neisseria gonorrhoeae MS11

44.416

84.989

0.377
  pilF Thermus thermophilus HB27

40.342

90.287

0.364