Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5O79_RS11200 Genome accession   NZ_CP104516
Coordinates   2193019..2193459 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain E4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2188019..2198459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O79_RS11180 (N5O79_11180) aroP 2189017..2190390 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  N5O79_RS11185 (N5O79_11185) ampE 2190433..2191287 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  N5O79_RS11190 (N5O79_11190) ampD 2191284..2191835 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5O79_RS11195 (N5O79_11195) nadC 2191923..2192816 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5O79_RS11200 (N5O79_11200) pilA 2193019..2193459 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  N5O79_RS11205 (N5O79_11205) pilB 2193493..2194854 (+) 1362 WP_261307162.1 type II secretion system protein GspE Machinery gene
  N5O79_RS11210 (N5O79_11210) hofC 2194844..2196046 (+) 1203 WP_000157266.1 protein transport protein HofC -
  N5O79_RS11215 (N5O79_11215) guaC 2196081..2197124 (-) 1044 WP_001217335.1 GMP reductase -
  N5O79_RS11220 (N5O79_11220) - 2197280..2197324 (-) 45 WP_120795372.1 protein YacM -
  N5O79_RS11225 (N5O79_11225) coaE 2197349..2197969 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=630164 N5O79_RS11200 WP_000360904.1 2193019..2193459(+) (pilA) [Escherichia coli strain E4]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=630164 N5O79_RS11200 WP_000360904.1 2193019..2193459(+) (pilA) [Escherichia coli strain E4]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363