Detailed information NTG0628843

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LO004_RS15435
• Genome accession: NZ_CP087331
• Coordinates: 3226529..3227647 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain LMG994
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3221529..3232647

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LO004_RS15425	-	3224645..3225337 (-)	693	WP_001108515.1	YggS family pyridoxal phosphate-dependent enzyme	-
LO004_RS15430	pilT	3225464..3226501 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
LO004_RS15435	pilU	3226529..3227647 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LO004_RS15440	fur	3227717..3228154 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
LO004_RS15445	-	3228266..3228664 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
LO004_RS15450	-	3228719..3229042 (-)	324	WP_000442581.1	RnfH family protein	-
LO004_RS15455	-	3229042..3230109 (-)	1068	WP_010590411.1	membrane protein	-
LO004_RS15460	-	3230262..3230717 (-)	456	WP_000782978.1	bacteriohemerythrin	-
LO004_RS15465	-	3230970..3231818 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=628843 LO004_RS15435 WP_000347036.1 3226529..3227647(+) (pilU) [Acinetobacter baumannii strain LMG994]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=628843 LO004_RS15435 WP_000347036.1 3226529..3227647(+) (pilU) [Acinetobacter baumannii strain LMG994]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACCGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAGGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392