Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOY68_RS27310 Genome accession   NZ_CP087205
Coordinates   6101179..6102879 (+) Length   566 a.a.
NCBI ID   WP_265040953.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-053     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6096179..6107879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY68_RS27290 (LOY68_27265) - 6096391..6099333 (+) 2943 WP_265040949.1 DUF748 domain-containing protein -
  LOY68_RS27295 (LOY68_27270) - 6099333..6099629 (+) 297 WP_265040950.1 DUF2845 domain-containing protein -
  LOY68_RS27300 (LOY68_27275) - 6099873..6100220 (-) 348 WP_163911782.1 BON domain-containing protein -
  LOY68_RS27305 (LOY68_27280) - 6100552..6100953 (-) 402 WP_265040952.1 pilin -
  LOY68_RS27310 (LOY68_27285) pilB 6101179..6102879 (+) 1701 WP_265040953.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY68_RS27315 (LOY68_27290) pilC 6102882..6104099 (+) 1218 WP_265040954.1 type II secretion system F family protein Machinery gene
  LOY68_RS27320 (LOY68_27295) pilD 6104101..6104973 (+) 873 WP_265040955.1 prepilin peptidase Machinery gene
  LOY68_RS27325 (LOY68_27300) coaE 6104970..6105593 (+) 624 WP_265040957.1 dephospho-CoA kinase -
  LOY68_RS27330 (LOY68_27305) yacG 6105590..6105790 (+) 201 WP_054051315.1 DNA gyrase inhibitor YacG -
  LOY68_RS27335 (LOY68_27310) - 6105813..6106028 (-) 216 WP_054051316.1 hypothetical protein -
  LOY68_RS27340 (LOY68_27315) - 6106092..6106781 (-) 690 WP_054051318.1 energy-coupling factor ABC transporter permease -
  LOY68_RS27345 (LOY68_27320) - 6106916..6107542 (+) 627 WP_008154887.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62690.87 Da        Isoelectric Point: 6.1334

>NTDB_id=628126 LOY68_RS27310 WP_265040953.1 6101179..6102879(+) (pilB) [Pseudomonas sp. B21-053]
MNDIALSGLAKQLVLAELLTEKSAQLAYQQAQRNRISLVSYLVQNKLAKSREVAEIASEHFGMALLDLHCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGVSDPTNHQAINDIQFSTGLSTEAILVEDDKLSDAIEKFFDSHATGLEEMADVDLDG
LDIETVDDRNHDSIPGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKALFMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFSQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVTLIIAQRLARKLCSHCKAPIDIPREALIKEGFPE
ERIGSFTIYEPVGCDHCNHGYKGRIGIYEVVKNTPDLQRLIMAEGNSLEIEIQMRKDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=628126 LOY68_RS27310 WP_265040953.1 6101179..6102879(+) (pilB) [Pseudomonas sp. B21-053]
ATGAACGACATCGCCCTTAGCGGTCTGGCCAAGCAATTAGTGCTGGCCGAACTGCTCACTGAAAAAAGCGCGCAACTGGC
GTATCAGCAAGCCCAGCGCAACCGGATTTCACTGGTCAGCTACCTGGTGCAGAACAAACTGGCGAAGAGCCGTGAAGTGG
CTGAGATTGCCTCGGAACATTTCGGCATGGCCCTGCTGGACCTCCACTGTCTGGACAAAGAGACCCAACCCAAGGGTCTG
GTCAGCGAGAAGCTGGTCCGCCAGCACCACGCCCTGCCCCTCTGGCGACGTGGCAATAAACTGTTTGTGGGGGTTTCCGA
CCCAACCAACCACCAGGCGATCAACGATATCCAGTTCAGCACAGGGCTGAGCACCGAAGCCATCCTGGTAGAGGACGACA
AGCTCAGCGACGCCATCGAAAAGTTCTTCGACAGCCACGCCACCGGCCTGGAAGAGATGGCCGATGTGGACCTCGATGGC
CTGGACATCGAGACCGTCGACGATAGAAACCACGACTCCATCCCCGGGCAAGACGCCGATGACGCGCCCGTGGTGCGCTT
CGTCCACAAGATGCTGCTTGATGCGATCAAGAGCGGCTCTTCCGACTTGCACTTCGAACCTTACGAAAAAACCTATCGGG
TACGGATGCGCACCGACGGCATCCTGCGCGAAGTCGCCAAGCCGCCGATTCAACTGGCCGGGCGGATTGCCGCCCGGCTC
AAGGTGATGGCCAGCCTCGACATTTCCGAAAGACGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAA
GTCAATCGACTTTCGGGTCAACACCTTGCCGACGCTGTGGGGCGAGAAAGTGGTGATTCGGATCCTCGACCCGTCCAGCG
CCCAGATGGGCATCGACGCCCTCGGTTACGAGCCCGACCAGAAAGCCCTGTTCATGGCCGCCCTCAAGCAGCCACAAGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAAACCGTGTCGCTCTACACCGGGCTGAATATTCTCAATACCGTGGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAAG
GGCTGGATTTCTCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTT
GAAACCGCGGAAATCGCGATCAAGGCCGCGCAGACCGGGCACTTGGTGCTGTCGACCCTGCACACCAACAGCGCTGCGGA
AACCCTGACCCGCCTGCATAACATGGGCATCCCGGGGTTCAACATCGCCACCTCGGTCACCCTGATCATCGCCCAGCGCC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGGCGCCGATCGACATCCCCCGCGAGGCGCTGATCAAGGAAGGCTTCCCTGAG
GAACGCATCGGCTCATTCACGATCTATGAGCCCGTCGGTTGCGATCACTGCAACCACGGCTACAAAGGGCGAATCGGGAT
TTATGAAGTGGTGAAGAACACGCCAGACCTGCAACGGCTGATCATGGCCGAAGGCAACTCCCTGGAAATCGAGATCCAGA
TGCGCAAGGACGGCTTCAACGACCTGCGCACTTCCGGGCTGATCAAGGCCATGCAAGGCATTACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.761

99.823

0.587
  pilB Acinetobacter baumannii D1279779

58.333

99.647

0.581
  pilB Legionella pneumophila strain ERS1305867

56.714

100

0.567
  pilB Vibrio cholerae strain A1552

50.973

99.823

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

50.817

97.35

0.495
  pilB Vibrio campbellii strain DS40M4

48.947

100

0.493
  pilF Neisseria gonorrhoeae MS11

49.201

99.47

0.489
  pilB/pilB1 Synechocystis sp. PCC 6803

37.05

98.233

0.364
  pilF Thermus thermophilus HB27

36.042

100

0.36