Detailed information NTG0628060

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LOY67_RS10930
• Genome accession: NZ_CP087203
• Coordinates: 2445236..2446351 (+)
• Length: 371 a.a.
• NCBI ID: WP_265067168.1
• Uniprot ID: -
• Organism: Pseudomonas sp. B21-056
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2440236..2451351

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOY67_RS10915 (LOY67_10910)	-	2441207..2442124 (+)	918	WP_265067166.1	sugar kinase	-
LOY67_RS10920 (LOY67_10915)	-	2442255..2442530 (-)	276	WP_003182808.1	peptidylprolyl isomerase	-
LOY67_RS10925 (LOY67_10920)	-	2442575..2445112 (-)	2538	WP_265067167.1	PAS domain-containing protein	-
LOY67_RS10930 (LOY67_10925)	pilU	2445236..2446351 (+)	1116	WP_265067168.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LOY67_RS10935 (LOY67_10930)	-	2446522..2448477 (-)	1956	WP_265067169.1	acetoacetate--CoA ligase	-
LOY67_RS10940 (LOY67_10935)	-	2448695..2449465 (-)	771	WP_265067748.1	3-hydroxybutyrate dehydrogenase	-
LOY67_RS10945 (LOY67_10940)	-	2449479..2450870 (-)	1392	WP_265067170.1	GntP family permease	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 41163.11 Da
• Isoelectric Point: 5.6945

>NTDB_id=628060 LOY67_RS10930 WP_265067168.1 2445236..2446351(+) (pilU) [Pseudomonas sp. B21-056]
MEIDPLLRILASQEGSDLYLSTGAPPCAKFEGVLKPLGNQVFKVGDIAGLAESLMDAEQRLEFDRELEMNLAISLSGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPKVLLETIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRSQLLSDLGNNLQAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDQAL
FNLVVEGVLDEEEALKNADSANNLRLRLKLYAESGAAPAADPAAGEWGLMD

Nucleotide

• Length: 1116 bp

>NTDB_id=628060 LOY67_RS10930 WP_265067168.1 2445236..2446351(+) (pilU) [Pseudomonas sp. B21-056]
ATGGAAATCGATCCCTTGTTACGCATCCTGGCCAGCCAGGAAGGCTCCGATCTTTACCTGTCTACCGGGGCGCCGCCGTG
TGCCAAGTTCGAGGGCGTGCTCAAGCCGTTGGGCAACCAGGTGTTCAAGGTCGGTGACATCGCCGGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAACTGGAAATGAACCTGGCGATTTCGTTGTCGGGTGTCGGGCGA
TTCCGGGTCAATATCTTCAAGCAGCGCAACGACGTGTCCATGGTGATTCGCAACGTCAAGCTGGACATCCCGCGCTTCGA
GGACTTGAAGCTGCCCAAGGTGCTGCTGGAGACCATCATGCAGAAGCAGGGGCTGATGCTGTTCGTCGGCGCGACGGGCT
CAGGCAAGTCCACCTCCCTGGCGGCATTGATCGATTACCGCAATCGCAACAGCAGCGGTCATATCATCACCATCGAGGAC
CCGGTGGAGTATATCCACCGGCACAAGAAGTCGATCATCAACCAGCGGGAAGTCGGTGTCGACACCCGCAGTTTTCATGC
AGCCCTGAAGAACACCCTGCGCCAGGCACCGGATGTGGTGCTGATCGGCGAAATTCGCGACCGGGAAACCATGGAACACG
CCCTGGCCTTTGCCGACACCGGGCACCTGGTGATCTCCACCTTGCATGCCCATAACGCCAATCAGGCGTTGGACCGGGTG
ATCAACTTCTTCCCGGAAGAACGCCGGTCGCAGTTGCTGAGCGATCTGGGCAACAACCTCCAGGCCTTCGTGTCCCAGCG
CCTGGTACGCACCCGTACCGGCCAGCGCCGCGCCGCGGTGGAGGTCATGCTTGGCTCGCCCACGGTGGCCGACCTGATCC
GGCGCAACGAGCTGGGTGAACTCAAGGGCATCATGGAGAAGTCGGAAGAACTGGGCATGCAGACCTTCGACCAGGCCCTG
TTCAACCTGGTGGTGGAAGGGGTGCTGGATGAGGAAGAAGCCCTGAAGAACGCCGATTCGGCGAACAACCTGCGGTTGCG
GTTGAAGTTGTATGCCGAGTCGGGCGCCGCGCCTGCGGCCGATCCGGCGGCGGGGGAGTGGGGGTTGATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (58-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.338	95.687	0.539
pilU	Acinetobacter baylyi ADP1	51.821	96.226	0.499
pilU	Vibrio cholerae strain A1552	51.714	94.34	0.488
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.237	98.922	0.388
pilT	Vibrio cholerae strain A1552	40.841	89.757	0.367
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.841	89.757	0.367
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.722	0.367
pilT	Pseudomonas aeruginosa PAK	39.244	92.722	0.364