Detailed information NTG0627926

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LOY50_RS10950
• Genome accession: NZ_CP087198
• Coordinates: 2454096..2455214 (+)
• Length: 372 a.a.
• NCBI ID: WP_258690831.1
• Uniprot ID: -
• Organism: Pseudomonas sp. B21-010
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2449096..2460214

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOY50_RS10935 (LOY50_10905)	-	2450095..2451012 (+)	918	WP_258690829.1	sugar kinase	-
LOY50_RS10940 (LOY50_10910)	-	2451102..2451377 (-)	276	WP_003182808.1	peptidylprolyl isomerase	-
LOY50_RS10945 (LOY50_10915)	-	2451426..2453963 (-)	2538	WP_258690830.1	PAS domain-containing sensor histidine kinase	-
LOY50_RS10950 (LOY50_10920)	pilU	2454096..2455214 (+)	1119	WP_258690831.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LOY50_RS10955 (LOY50_10925)	-	2455333..2457288 (-)	1956	WP_258690832.1	acetoacetate--CoA ligase	-
LOY50_RS10960 (LOY50_10930)	hbdH	2457447..2458223 (-)	777	WP_063321984.1	3-hydroxybutyrate dehydrogenase	-
LOY50_RS10965 (LOY50_10935)	-	2458231..2459622 (-)	1392	WP_253473456.1	GntP family permease	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41396.45 Da
• Isoelectric Point: 6.3540

>NTDB_id=627926 LOY50_RS10950 WP_258690831.1 2454096..2455214(+) (pilU) [Pseudomonas sp. B21-010]
MEIDPLLRILASQNGSDLYMSTGAPPCARFEGVIKPLGNEAFKVGDIARLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPRVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLIDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDHAL
FNLVVEGAIDEEEALKNADSANNLRLRLKLHSESGAAAPPVDPAAGEWGLMD

Nucleotide

• Length: 1119 bp

>NTDB_id=627926 LOY50_RS10950 WP_258690831.1 2454096..2455214(+) (pilU) [Pseudomonas sp. B21-010]
ATGGAAATCGATCCGTTGTTGCGAATCCTGGCGAGCCAGAATGGTTCCGACCTTTACATGTCCACGGGTGCGCCACCGTG
CGCGCGCTTCGAGGGGGTGATCAAGCCCTTGGGTAACGAGGCGTTCAAGGTCGGCGACATTGCCAGGCTCGCCGAGTCTC
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAGTTGGAAATGAACCTGGCGATCTCCCTGGCCGGTGTCGGGCGG
TTCCGGGTCAATATCTTCAAGCAGCGCAATGACGTGTCCATGGTGATTCGCAACGTCAAGCTGGACATCCCGCGCTTCGA
AGACCTGAAACTGCCTCGAGTGCTTTTAGACACCATCATGCAGAAACAGGGGCTGATGCTGTTCGTCGGTGCGACAGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGCAATCGCAACAGCAGCGGCCACATCATCACCATCGAGGAC
CCGGTGGAGTATATCCATCGGCACAAGAAATCCATCATCAATCAACGGGAAGTCGGTGTCGACACCCGCAGTTTCCATGC
AGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAAATCCGCGACCGGGAAACCATGGAGCATG
CCTTGGCGTTTGCCGACACCGGGCACCTGGTGATTTCCACCTTGCATGCCCATAACGCCAACCAGGCCCTGGACCGGGTG
ATCAATTTCTTTCCGGAAGAACGCCGGCCGCAGTTGCTCATTGACCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
CCTGGTGCGTACCCGCACCGGCCAGCGACGAGCGGCGGTGGAGGTCATGCTGGGCTCGCCGACGGTGGCCGACCTGATCC
GGCGCAACGAGCTCGGCGAGCTCAAGGGCATCATGGAGAAGTCAGAGGAACTGGGGATGCAAACCTTTGATCACGCGCTG
TTCAACCTGGTGGTCGAAGGTGCCATCGATGAGGAAGAAGCGCTGAAGAATGCCGACTCGGCGAACAACCTGCGGTTGCG
GTTGAAGTTGCATAGCGAGTCGGGGGCGGCGGCGCCACCGGTCGATCCGGCGGCGGGTGAGTGGGGGTTGATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.901	95.43	0.543
pilU	Acinetobacter baylyi ADP1	51.648	97.849	0.505
pilU	Vibrio cholerae strain A1552	52.571	94.086	0.495
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.525	98.387	0.379
pilT	Vibrio cholerae strain A1552	40.841	89.516	0.366
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.841	89.516	0.366
pilT	Pseudomonas stutzeri DSM 10701	39.244	92.473	0.363
pilT	Pseudomonas aeruginosa PAK	38.953	92.473	0.36