Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY24_RS26915 Genome accession   NZ_CP087183
Coordinates   6021163..6022431 (-) Length   422 a.a.
NCBI ID   WP_258654267.1    Uniprot ID   -
Organism   Pseudomonas putida strain B21-029     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6016163..6027431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY24_RS26900 (LOY24_26875) - 6017825..6019429 (-) 1605 WP_258654265.1 SPOR domain-containing protein -
  LOY24_RS26905 (LOY24_26880) aroB 6019445..6020542 (-) 1098 WP_258654266.1 3-dehydroquinate synthase -
  LOY24_RS26910 (LOY24_26885) aroK 6020640..6021158 (-) 519 WP_024717571.1 shikimate kinase AroK -
  LOY24_RS26915 (LOY24_26890) pilQ 6021163..6022431 (-) 1269 WP_258654267.1 type IV pilus secretin PilQ Machinery gene
  LOY24_RS26920 (LOY24_26895) - 6022355..6023356 (-) 1002 WP_258657789.1 pilus assembly protein PilP -
  LOY24_RS26925 (LOY24_26900) - 6023368..6023904 (-) 537 WP_258654268.1 PilN domain-containing protein -
  LOY24_RS26930 (LOY24_26905) pilM 6023901..6024782 (-) 882 WP_258654269.1 type IV pilus biogenesis protein PilM -
  LOY24_RS26935 (LOY24_26910) - 6024973..6027426 (+) 2454 WP_258654270.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45975.76 Da        Isoelectric Point: 4.8332

>NTDB_id=627534 LOY24_RS26915 WP_258654267.1 6021163..6022431(-) (pilQ) [Pseudomonas putida strain B21-029]
MKRVMKWMLAALLPIPLVWATPYQGEPLSLNFQDVEVRSVLQVLADYAGVNLVASDDVQGNVTLRLQDVPWDQALDLVLR
SKGLARQQEGNVLLVAPAAAFAPQSFDAHPGLLPDAQLQPLLRELIPIHHANAADLVELLMTSLADDSTLAVRGSLGVDE
RTNTLVAHQSAERLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGENLRPGKELFIDLGVERAGSSV
GLGLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDN
KVIMAVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFLGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=627534 LOY24_RS26915 WP_258654267.1 6021163..6022431(-) (pilQ) [Pseudomonas putida strain B21-029]
ATGAAGAGAGTGATGAAATGGATGTTGGCAGCCCTGCTGCCGATCCCGCTGGTGTGGGCGACGCCTTACCAGGGTGAACC
CCTGTCGTTGAATTTCCAGGATGTGGAGGTGCGTTCGGTGCTGCAGGTGCTGGCCGATTATGCGGGCGTCAACCTGGTTG
CCAGCGATGACGTGCAGGGCAACGTGACCTTGCGGTTGCAAGACGTGCCCTGGGACCAGGCGCTTGACCTGGTGCTGCGC
AGCAAGGGCCTGGCTCGGCAGCAAGAGGGCAATGTGCTGCTGGTGGCGCCTGCGGCAGCGTTTGCCCCGCAGTCGTTCGA
TGCCCATCCGGGCCTGTTGCCGGATGCGCAATTGCAGCCGTTACTCCGTGAATTGATACCGATCCACCATGCCAACGCTG
CCGATCTGGTTGAGCTGTTGATGACCAGCCTGGCGGATGACAGCACACTCGCCGTTCGTGGCAGCCTGGGTGTCGATGAG
CGCACCAACACCTTGGTGGCGCACCAGTCCGCCGAGCGCCTGGCCGAATTGCGGCAACTGGTTGCGCAGCTGGATGTGCC
GGTGCGCCAGGTGGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTACGAAAAAAGCCTGGGGGTGCGCTGGGGCG
GGCCGTTGTACGGTGAAAACCTGCGCCCGGGCAAGGAACTGTTCATCGACCTGGGCGTGGAACGGGCGGGTAGCAGCGTC
GGCCTGGGACTGCTGCGCGGCGACGTGCTGCTCGACCTGGAGCTCAGTGCCATGGAAAAAAGCGGCAACGGCGAAATCAT
CTCGCAGCCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAAGGCACCGAGGTGCCTTACCAGGAAACCA
GCAAGAGCGGCGCCACCTCGGTCGCCTTTCGCGAAGCCTCGCTGTCGCTGGAGGTGACCCCGCAAATTACACCGGACAAC
AAGGTGATCATGGCGGTCAGGGTGACCAAGGATGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAA
GAACGAGGTGAACGCCAAGGTTCGCGTGGCGGACGGTGAAACGATCGTGATCGGTGGCGTGTACTCGACTTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTAGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(50-97)

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.77

100

0.611
  comQ Acinetobacter baylyi ADP1

42.444

100

0.453
  pilQ Acinetobacter baumannii D1279779

40.948

100

0.45
  pilQ Acinetobacter baumannii strain A118

40.948

100

0.45
  pilQ Legionella pneumophila strain ERS1305867

42.105

99.052

0.417
  pilQ Legionella pneumophila strain Lp02

42.105

99.052

0.417
  pilQ Vibrio campbellii strain DS40M4

41.038

100

0.412
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.587

98.578

0.41
  pilQ Vibrio cholerae strain A1552

41.587

98.578

0.41