Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY33_RS24265 Genome accession   NZ_CP087178
Coordinates   5327233..5328504 (-) Length   423 a.a.
NCBI ID   WP_408980710.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-036     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5322233..5333504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY33_RS24250 (LOY33_24220) - 5323900..5325495 (-) 1596 WP_258660327.1 AAA family ATPase -
  LOY33_RS24255 (LOY33_24225) aroB 5325512..5326609 (-) 1098 WP_043859938.1 3-dehydroquinate synthase -
  LOY33_RS24260 (LOY33_24230) aroK 5326710..5327228 (-) 519 WP_043859939.1 shikimate kinase AroK -
  LOY33_RS24265 (LOY33_24235) pilQ 5327233..5328504 (-) 1272 WP_408980710.1 type IV pilus secretin PilQ Machinery gene
  LOY33_RS24270 (LOY33_24240) - 5328529..5329434 (-) 906 WP_258660331.1 pilus assembly protein PilP -
  LOY33_RS24275 (LOY33_24245) - 5329431..5329961 (-) 531 WP_168186835.1 PilN domain-containing protein -
  LOY33_RS24280 (LOY33_24250) pilM 5329958..5330809 (-) 852 WP_258660333.1 type IV pilus biogenesis protein PilM -
  LOY33_RS24285 (LOY33_24255) - 5330984..5333416 (+) 2433 WP_196932068.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 45070.72 Da        Isoelectric Point: 4.9740

>NTDB_id=627417 LOY33_RS24265 WP_408980710.1 5327233..5328504(-) (pilQ) [Pseudomonas sp. B21-036]
MFSLCAVLMVALPVAGAGLGAEGAGYGGEPLSLSFQDVEVRSVLKVLADFTGVNLVASDAVQGKVTLRLHEVPWEQALDL
VLRSKGLGRRQEGNVLLVAPLAELAGHDTQARAAQAPPTLVRELLTVSHARAGDIVQLFQAMVENDDPGAITVDERTNTI
IASQPSARLADLRRLLAQLDVPVRQVMIEARIVEANVDYEKSLGVRWGGAVQLGGGLRAGGLAADGLPFVDLGVERATSG
IGLGLVRDSVLLDLELSAMEKTGNGEIISQPKVVTADKETARILKGTEVPYQESGANGTTSTTFREASLSLEVTPQITAD
NRVIMTVKVTKDEPDYLNALNSVPPIRKNEVNAKVQVGDGETIVIGGVYATVQSNVVDKVPFFGDVPYVGRLFRRAVIQE
KKSELLVFLTPRIMSDRAIAVSR

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=627417 LOY33_RS24265 WP_408980710.1 5327233..5328504(-) (pilQ) [Pseudomonas sp. B21-036]
ATGTTCAGCCTGTGTGCGGTACTGATGGTGGCGCTGCCGGTAGCAGGTGCAGGCCTTGGGGCCGAGGGGGCAGGGTATGG
CGGCGAGCCGCTGTCGTTGAGTTTCCAGGATGTCGAGGTGCGTTCGGTGCTCAAGGTGCTGGCCGACTTCACCGGGGTCA
ACCTGGTGGCCAGTGATGCAGTACAGGGCAAGGTGACCCTGCGCCTGCACGAGGTGCCCTGGGAACAGGCACTGGACCTG
GTGCTGCGCAGCAAGGGCCTGGGTCGGCGTCAGGAGGGCAACGTGCTGCTGGTGGCGCCTTTGGCCGAACTGGCCGGGCA
CGATACCCAGGCCCGTGCGGCGCAGGCCCCGCCAACGCTGGTGCGCGAACTGCTCACGGTCAGCCATGCGCGGGCCGGGG
ACATCGTGCAGCTGTTCCAGGCCATGGTCGAGAACGACGACCCCGGCGCGATCACGGTCGACGAGCGCACCAACACCATC
ATTGCCAGCCAGCCGTCGGCACGCCTGGCCGACCTGCGGCGGCTGCTCGCGCAGCTCGATGTACCGGTTCGCCAGGTGAT
GATCGAGGCGCGCATCGTCGAGGCCAATGTCGATTACGAAAAAAGCCTGGGGGTGCGCTGGGGCGGGGCGGTGCAACTGG
GCGGCGGCCTGCGCGCAGGCGGGTTGGCCGCCGACGGGCTGCCGTTCGTCGACCTGGGGGTGGAACGGGCCACCTCGGGT
ATCGGCCTGGGGTTGGTGCGCGACAGTGTGCTGCTCGACCTGGAGCTCAGCGCGATGGAAAAGACCGGCAATGGCGAAAT
CATCTCCCAGCCCAAGGTGGTCACCGCCGACAAGGAAACCGCGCGCATTCTCAAGGGTACCGAGGTGCCGTATCAGGAGT
CGGGCGCCAACGGCACTACGTCCACCACCTTTCGCGAGGCCTCGCTGTCGCTGGAGGTGACCCCGCAGATCACGGCCGAC
AACCGGGTGATCATGACGGTCAAGGTCACCAAGGACGAGCCGGACTACCTCAATGCCCTCAACAGCGTGCCGCCGATCCG
CAAGAACGAGGTGAATGCCAAGGTTCAGGTGGGGGATGGCGAGACCATCGTGATCGGCGGGGTGTATGCCACCGTGCAAA
GTAATGTTGTCGACAAGGTGCCATTTTTCGGCGATGTGCCGTATGTTGGGCGGCTGTTTCGTCGCGCTGTCATCCAAGAG
AAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCGGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(54-100)

Bacterial type II and III secretion system protein (Secretin)

(258-414)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-185)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.817

100

0.619
  pilQ Acinetobacter baumannii D1279779

41.215

100

0.449
  pilQ Acinetobacter baumannii strain A118

41.215

100

0.449
  comQ Acinetobacter baylyi ADP1

40.94

100

0.433
  pilQ Legionella pneumophila strain ERS1305867

40.385

98.345

0.397
  pilQ Legionella pneumophila strain Lp02

40.385

98.345

0.397
  pilQ Vibrio campbellii strain DS40M4

39.059

100

0.392
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.713

98.818

0.392
  pilQ Vibrio cholerae strain A1552

39.713

98.818

0.392