Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LOY27_RS12340 Genome accession   NZ_CP087171
Coordinates   2755517..2756626 (+) Length   369 a.a.
NCBI ID   WP_130900060.1    Uniprot ID   -
Organism   Pseudomonas atacamensis strain B21-045     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2750517..2761626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY27_RS12325 (LOY27_12295) - 2751502..2752425 (+) 924 WP_258711589.1 sugar kinase -
  LOY27_RS12330 (LOY27_12300) - 2752542..2752817 (-) 276 WP_007912322.1 peptidylprolyl isomerase -
  LOY27_RS12335 (LOY27_12305) - 2752858..2755407 (-) 2550 WP_258711590.1 PAS domain-containing protein -
  LOY27_RS12340 (LOY27_12310) pilU 2755517..2756626 (+) 1110 WP_130900060.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LOY27_RS12345 (LOY27_12315) - 2756805..2760458 (+) 3654 WP_258711591.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40826.84 Da        Isoelectric Point: 6.9861

>NTDB_id=627226 LOY27_RS12340 WP_130900060.1 2755517..2756626(+) (pilU) [Pseudomonas atacamensis strain B21-045]
MEIDALLSILSQKNGSDLFLSTGAAPSARIDGVLTALSDQPFKPGETAAIATSLMDAEQRREFDRDLEMNLAISRTGVGR
FRVNIFKQRNDVSIVIRNVKVDIPRFEDLKLPPVLLETVMLKRGLILFVGATSSGKSTSLAALIDHRNRHSAGHIVTIED
PIEYIHRHKRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALSFADTGHLVLSTLHATNANQALDRI
INMFPEEKREQLLQTLGNNLKACVSQRLLRTVDGQRRAAVEVLLGTPTVADALRRNELAELKSIMEKSEDSGMQTFDAAL
LQLVLARVITVEEALKNADSINNLRLGIKLKLDSPINEKASTGDWGLVE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=627226 LOY27_RS12340 WP_130900060.1 2755517..2756626(+) (pilU) [Pseudomonas atacamensis strain B21-045]
ATGGAAATCGATGCACTGTTGTCAATATTGTCGCAAAAAAACGGCTCGGATCTGTTCCTCTCTACCGGCGCAGCGCCGAG
CGCGCGCATTGATGGCGTGCTGACAGCGCTCAGCGATCAGCCATTCAAACCCGGCGAAACCGCGGCTATCGCCACCTCCC
TGATGGACGCCGAGCAGCGCCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCAATCTCCCGAACGGGCGTCGGGCGG
TTCCGGGTGAACATTTTCAAGCAGCGCAACGACGTTTCGATCGTGATCCGCAACGTCAAGGTCGACATCCCGCGATTCGA
AGACCTGAAGCTGCCGCCGGTGCTGCTGGAAACGGTGATGCTCAAACGTGGCCTGATTCTTTTTGTCGGCGCGACCAGCT
CAGGCAAGTCGACTTCACTGGCGGCACTCATCGATCACCGCAACCGTCACAGTGCCGGACACATTGTGACGATCGAAGAC
CCGATCGAATATATCCATCGGCACAAGCGCTCGATCATCAATCAGCGTGAAGTCGGCGTAGATACCCGAAGCTTTCATGC
GGCTTTGAAGAACACTCTGCGCCAGGCTCCGGATGTTGTATTGATTGGTGAGATCCGTGACCGCGAAACCATGGAGCATG
CACTGTCATTCGCTGATACCGGCCATCTTGTGTTGTCGACGCTGCATGCGACCAACGCCAATCAGGCGCTGGATCGAATC
ATCAATATGTTTCCCGAGGAGAAGCGCGAACAGCTTTTGCAGACATTGGGCAACAACCTCAAAGCCTGTGTTTCGCAGCG
TCTGCTCCGCACTGTGGACGGCCAGCGCAGGGCGGCCGTGGAAGTGTTATTAGGTACGCCGACTGTTGCTGATGCGCTCC
GTCGCAATGAGTTGGCTGAGTTGAAAAGTATCATGGAGAAATCCGAAGACTCAGGCATGCAGACATTCGATGCTGCACTT
TTACAGCTAGTCCTTGCGCGTGTTATTACAGTAGAAGAAGCTTTGAAAAACGCGGATTCGATAAACAACTTACGGTTAGG
CATCAAGCTTAAACTTGATTCTCCAATCAACGAAAAAGCATCGACAGGGGATTGGGGGTTGGTGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.556

95.122

0.528
  pilU Acinetobacter baylyi ADP1

52.841

95.393

0.504
  pilU Vibrio cholerae strain A1552

51.136

95.393

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.298

91.87

0.379
  pilT Neisseria meningitidis 8013

39.296

92.412

0.363
  pilT Neisseria gonorrhoeae MS11

39.296

92.412

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.24

90.244

0.363
  pilT Vibrio cholerae strain A1552

40.24

90.244

0.363