Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY26_RS25125 Genome accession   NZ_CP087169
Coordinates   5566219..5567487 (-) Length   422 a.a.
NCBI ID   WP_258714217.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-047     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5561219..5572487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY26_RS25110 (LOY26_25085) - 5562861..5564486 (-) 1626 WP_033702003.1 SPOR domain-containing protein -
  LOY26_RS25115 (LOY26_25090) aroB 5564502..5565599 (-) 1098 WP_047582202.1 3-dehydroquinate synthase -
  LOY26_RS25120 (LOY26_25095) aroK 5565696..5566214 (-) 519 WP_033702005.1 shikimate kinase AroK -
  LOY26_RS25125 (LOY26_25100) pilQ 5566219..5567487 (-) 1269 WP_258714217.1 type IV pilus secretin PilQ Machinery gene
  LOY26_RS25130 (LOY26_25105) - 5567411..5568409 (-) 999 WP_186652916.1 pilus assembly protein PilP -
  LOY26_RS25135 (LOY26_25110) - 5568424..5568960 (-) 537 WP_186652918.1 PilN domain-containing protein -
  LOY26_RS25140 (LOY26_25115) pilM 5568957..5569820 (-) 864 WP_186652920.1 type IV pilus biogenesis protein PilM -
  LOY26_RS25145 (LOY26_25120) - 5570002..5572452 (+) 2451 WP_371856397.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46010.90 Da        Isoelectric Point: 5.2074

>NTDB_id=627189 LOY26_RS25125 WP_258714217.1 5566219..5567487(-) (pilQ) [Pseudomonas sp. B21-047]
MKKLWKWMVAWVLAIPLASATPYRGEPLSLNFQDVQVRAVLQVLADYAGVNLVASDAVQGNITLRLQDVPWDQALDLVLR
SKGLARREEGNVLLVAPAAELAGQSLDARMGQVLDAQLQPLQRELMPIHHAEASALAQLLLATLADDGILTGRGSLSVDV
RTNTLVAYQPADRLDELRQLVAQLDVPVRQVAIEARIVEASVDYEKSLGVRWGGPLYGDRVRPGKELFVDLGVERAGSSV
GLGVLRGGILLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVSFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDVLQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=627189 LOY26_RS25125 WP_258714217.1 5566219..5567487(-) (pilQ) [Pseudomonas sp. B21-047]
ATGAAGAAGCTCTGGAAATGGATGGTGGCGTGGGTCTTGGCGATCCCGCTAGCGTCGGCAACGCCCTACCGGGGTGAGCC
GCTGTCGCTGAACTTTCAGGATGTGCAGGTGCGCGCGGTGTTGCAGGTGCTGGCGGACTACGCCGGTGTGAACCTGGTCG
CCAGCGACGCGGTGCAGGGCAATATCACCCTGCGGCTTCAGGATGTGCCCTGGGACCAGGCGCTTGATCTGGTGCTGCGT
AGCAAGGGCCTGGCCCGTCGCGAAGAGGGTAATGTGCTGCTGGTGGCGCCTGCGGCCGAGCTTGCCGGGCAGTCGCTCGA
CGCCCGCATGGGCCAGGTGCTGGATGCCCAGTTGCAACCCCTGCAGCGTGAGCTGATGCCGATCCATCACGCCGAAGCTT
CAGCGCTCGCGCAACTGCTGCTGGCGACACTGGCGGACGACGGCATCCTGACCGGGCGAGGCAGTTTGAGCGTCGATGTG
CGCACCAACACCCTGGTGGCCTACCAACCCGCCGATCGCCTTGACGAGCTGCGCCAACTGGTGGCGCAGCTGGATGTGCC
GGTGCGCCAGGTGGCGATCGAGGCGCGTATTGTCGAGGCCAGCGTCGATTACGAAAAGAGCCTGGGGGTGCGTTGGGGTG
GGCCTTTGTACGGTGACCGCGTGCGGCCAGGCAAGGAGCTGTTCGTCGACCTTGGCGTGGAGCGTGCCGGCTCCAGTGTG
GGCCTGGGTGTGCTGCGCGGGGGCATCTTGCTCGACCTTGAACTCAGTGCCATGGAGAAGAGCGGCAATGGCGAAATCAT
CTCGCAGCCGAAGGTGGTCACGGCCGACAAGGAAACCGCCAGGATCCTCAAGGGCACTGAAGTGCCCTACCAGGAAACCA
GCAAGAGCGGCGCCACTTCGGTGTCGTTTCGCGAGGCTTCGCTGTCGCTCGAAGTCACCCCGCAAATCACCCCGGACAAC
AAGGTCATCATGACCGTCAGGGTAACCAAGGACGAGCCTGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAA
GAACGAGGTCAATGCCAAGGTTCGCGTGGCCGACGGCGAGACGATCGTGATCGGTGGTGTCTACTCGACCACGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGTATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.862

100

0.618
  pilQ Acinetobacter baumannii D1279779

42.082

100

0.46
  pilQ Acinetobacter baumannii strain A118

42.082

100

0.46
  comQ Acinetobacter baylyi ADP1

42.667

100

0.455
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.548

98.578

0.419
  pilQ Vibrio cholerae strain A1552

42.548

98.578

0.419
  pilQ Legionella pneumophila strain Lp02

42.344

99.052

0.419
  pilQ Legionella pneumophila strain ERS1305867

42.344

99.052

0.419
  pilQ Vibrio campbellii strain DS40M4

39.583

100

0.405