Detailed information NTG0627089

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LOY52_RS12255
• Genome accession: NZ_CP087166
• Coordinates: 2748644..2749756 (+)
• Length: 370 a.a.
• NCBI ID: WP_258679860.1
• Uniprot ID: -
• Organism: Pseudomonas sp. B21-051
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2743644..2754756

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOY52_RS12240 (LOY52_12220)	-	2744602..2745537 (+)	936	WP_258679644.1	sugar kinase	-
LOY52_RS12245 (LOY52_12225)	-	2745646..2745921 (-)	276	WP_003225447.1	peptidylprolyl isomerase	-
LOY52_RS12250 (LOY52_12230)	-	2745961..2748510 (-)	2550	WP_258679645.1	PAS domain-containing sensor histidine kinase	-
LOY52_RS12255 (LOY52_12235)	pilU	2748644..2749756 (+)	1113	WP_258679860.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LOY52_RS12260 (LOY52_12240)	-	2749867..2751822 (-)	1956	WP_258679646.1	acetoacetate--CoA ligase	-
LOY52_RS12265 (LOY52_12245)	hbdH	2751990..2752763 (-)	774	WP_258679647.1	3-hydroxybutyrate dehydrogenase	-
LOY52_RS12270 (LOY52_12250)	-	2752771..2754162 (-)	1392	WP_258679648.1	GntP family permease	-

Sequence

Protein

• Length: 370 a.a.
• Molecular weight: 41140.99 Da
• Isoelectric Point: 6.6255

>NTDB_id=627089 LOY52_RS12255 WP_258679860.1 2748644..2749756(+) (pilU) [Pseudomonas sp. B21-051]
MEIDALLLRLSSQHGSDLFLSTGAPPSARIDGVLTPFSERPFKPGEVETIANSLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHQRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALTFADTGHLVLSTLHAHNANQALDRI
VNLFPEERRPQLLHALGNNLKAFVSQRLVRTLDGQRRAAVEVMLGTPTIADLIRRNELGELKGIMEKSGEVGMQTFDAAL
YGLVVEGVISEDEALKHADSQNNLRLRLKLHAEAGPAPAPSSSGEWGLMD

Nucleotide

• Length: 1113 bp

>NTDB_id=627089 LOY52_RS12255 WP_258679860.1 2748644..2749756(+) (pilU) [Pseudomonas sp. B21-051]
ATGGAAATCGATGCACTGTTGCTCCGTCTGTCGAGCCAGCACGGCTCTGACCTGTTCCTGTCCACGGGAGCGCCACCCAG
TGCGCGCATCGATGGCGTGCTGACGCCGTTCAGCGAGCGGCCGTTCAAGCCCGGCGAGGTCGAGACCATCGCCAACTCCC
TGATGGACGCCGAGCAACGGCGCGAGTTCGATCGGGATCTGGAAATGAACCTGGCAATTTCCCGTACGGGCATCGGGCGT
TTTCGGGTGAACATCTTCAAGCAACGCAATGATGTGTCAATCGTGATCCGCAACGTCAAACTCGATATCCCGCGCTTCGA
AGACCTGAAACTGCCGCCGGTACTGCTGGAAACGGTAATGCTCAAACAAGGGCTGATCCTGTTCGTTGGTGCCACCGACT
CCGGCAAATCCACCTCGCTGGCCGCATTGATCGATCACCGCAATCGCCACAGCAGCGGGCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGACACCAGCGCTCGATCATCAATCAACGCGAAGTCGGCGTCGATACCCGCAGTTTCCATGC
GGCGCTGAAAAACACCCTGCGTCAGGCCCCGGACGTGGTGCTGATCGGCGAGATCCGCGACCGCGAAACCATGGAGCATG
CGCTGACATTTGCCGACACCGGGCACCTGGTGCTGTCGACGCTGCATGCGCACAATGCCAATCAGGCGCTGGACCGTATC
GTCAATCTGTTCCCCGAAGAGCGCCGCCCGCAGTTGCTGCATGCGCTGGGCAACAATCTCAAGGCGTTCGTCTCGCAACG
GCTGGTGCGTACGCTGGACGGCCAGCGCCGGGCGGCGGTCGAGGTGATGCTCGGCACGCCGACCATCGCCGACCTGATCC
GCCGCAACGAGTTGGGCGAACTCAAGGGCATCATGGAGAAGTCCGGCGAGGTCGGCATGCAGACATTCGACGCGGCGCTG
TACGGGCTGGTGGTGGAGGGCGTGATCAGTGAGGACGAAGCGCTCAAACATGCCGATTCGCAAAATAATCTGCGCCTGCG
TTTGAAGCTGCACGCCGAGGCGGGCCCGGCGCCCGCGCCTTCATCATCAGGCGAGTGGGGCCTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (68-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.62	95.946	0.543
pilU	Acinetobacter baylyi ADP1	53.221	96.486	0.514
pilU	Vibrio cholerae strain A1552	52.857	94.595	0.5
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.018	90.27	0.37
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	39.595	93.514	0.37
pilT	Vibrio cholerae strain A1552	39.595	93.514	0.37
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.973	0.368
pilT	Pseudomonas aeruginosa PAK	39.244	92.973	0.365