Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   LMH63_RS04385 Genome accession   NZ_CP086615
Coordinates   954153..955382 (-) Length   409 a.a.
NCBI ID   WP_109675914.1    Uniprot ID   A0A2U2N7Q2
Organism   Spiribacter halobius strain E85     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 949153..960382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH63_RS04360 (LMH63_04360) - 950055..951047 (+) 993 WP_229332725.1 Nudix family hydrolase -
  LMH63_RS04365 (LMH63_04365) yacG 951577..951774 (-) 198 WP_109675922.1 DNA gyrase inhibitor YacG -
  LMH63_RS04370 (LMH63_04370) zapD 951777..952565 (-) 789 WP_109675920.1 cell division protein ZapD -
  LMH63_RS04375 (LMH63_04375) coaE 952632..953228 (-) 597 WP_109675918.1 dephospho-CoA kinase -
  LMH63_RS04380 (LMH63_04380) pilD 953232..954101 (-) 870 WP_109675916.1 A24 family peptidase Machinery gene
  LMH63_RS04385 (LMH63_04385) pilC 954153..955382 (-) 1230 WP_109675914.1 type II secretion system F family protein Machinery gene
  LMH63_RS04390 (LMH63_04390) pilB 955396..957114 (-) 1719 WP_109675912.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LMH63_RS04395 (LMH63_04395) - 957324..959120 (-) 1797 WP_109675910.1 EAL domain-containing protein -
  LMH63_RS04400 (LMH63_04400) - 959276..959554 (-) 279 WP_109676149.1 HU family DNA-binding protein -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44629.30 Da        Isoelectric Point: 10.0805

>NTDB_id=625713 LMH63_RS04385 WP_109675914.1 954153..955382(-) (pilC) [Spiribacter halobius strain E85]
MAEKAAKLASFTWEGVDRQGRKVKGDSQAENPSRLRLTLRQQGIAAQKIRRKSSLSQLLEGKRSKKITPGDIAVFTRQLS
TMLNSGVPLVQAFDIIGRGHENPSMRKLVMAIKDDVESGTALSEALGKHPLYFDELVCNLVNAGEQAGVLDTLLDKIATY
KEKTESIKKKIRKALFYPTAVLVVAFAVTAILLIFVVPQFESLFRGFGADLPAFTRMVLNLSDFFRAWWWAIFGGLIGGV
VLFLEAKKRSRRFAHFLDRVALRIPVVGSILRKAAIARFARTLSTMFAAGVPLVEALGSVAGATGNAVYGEAVRGMQDEV
ATGQQLQWSVEQTKLFPHMVVQMIAIGEEAGSLDAMLAKVADFYEEEVDNAIDSLSSLLEPLIMAVLGVLVGGLVIAMYL
PIFKLGSVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=625713 LMH63_RS04385 WP_109675914.1 954153..955382(-) (pilC) [Spiribacter halobius strain E85]
ATGGCCGAGAAAGCAGCGAAGCTCGCAAGCTTCACCTGGGAGGGCGTCGACCGTCAGGGTCGCAAGGTCAAGGGCGACAG
CCAGGCGGAGAATCCGTCCCGGCTGCGCCTGACGCTGCGCCAGCAGGGCATCGCCGCGCAGAAGATCCGCCGCAAGTCGA
GCCTCTCGCAGCTGCTCGAGGGCAAGCGCAGCAAGAAGATCACCCCCGGCGACATCGCCGTGTTCACCCGCCAGCTCTCC
ACCATGCTGAACTCCGGCGTGCCGCTGGTGCAGGCCTTCGACATCATCGGACGGGGCCACGAGAACCCGTCCATGCGCAA
GCTGGTGATGGCGATCAAGGACGACGTCGAGTCCGGCACCGCGCTCTCCGAGGCCCTCGGCAAGCATCCGCTCTACTTTG
ACGAGCTGGTCTGCAACCTGGTCAACGCCGGCGAGCAGGCGGGCGTGCTGGACACGCTGCTGGACAAGATCGCGACCTAC
AAGGAAAAAACCGAGTCGATCAAGAAGAAGATCCGCAAGGCCCTGTTCTACCCCACCGCCGTGCTGGTGGTGGCGTTCGC
GGTAACGGCCATCCTGCTGATCTTCGTCGTGCCGCAGTTCGAGAGCCTGTTCCGCGGCTTCGGCGCCGACCTGCCGGCCT
TCACCCGGATGGTGCTGAATCTCTCGGACTTCTTCCGCGCCTGGTGGTGGGCGATCTTCGGCGGGCTGATCGGCGGGGTG
GTGCTCTTCCTAGAGGCGAAGAAACGCTCCCGGCGCTTCGCTCACTTTCTCGACCGGGTGGCCCTGCGCATCCCGGTGGT
GGGCAGCATCCTGCGCAAGGCGGCCATTGCCCGCTTCGCCCGCACCCTCTCCACCATGTTCGCCGCCGGCGTGCCGCTGG
TGGAGGCGCTGGGCTCGGTAGCCGGCGCCACGGGCAACGCCGTGTACGGCGAGGCCGTGCGGGGCATGCAGGACGAGGTC
GCCACCGGCCAGCAGCTGCAGTGGTCGGTGGAGCAGACCAAGCTCTTCCCGCACATGGTGGTGCAGATGATCGCCATCGG
CGAGGAGGCCGGTTCCCTCGACGCCATGCTTGCCAAGGTCGCCGACTTCTACGAGGAGGAGGTGGACAACGCCATCGACA
GCCTGAGCAGCCTGCTGGAGCCGTTGATCATGGCGGTGCTCGGCGTGCTCGTGGGCGGCCTGGTCATCGCCATGTACCTG
CCGATCTTCAAGCTCGGCTCGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U2N7Q2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

59.902

100

0.599
  pilC Pseudomonas stutzeri DSM 10701

58.191

100

0.582
  pilC Acinetobacter baylyi ADP1

54.015

100

0.543
  pilC Acinetobacter baumannii D1279779

54.3

99.511

0.54
  pilG Neisseria meningitidis 44/76-A

47.291

99.267

0.469
  pilG Neisseria gonorrhoeae MS11

46.798

99.267

0.465
  pilC Vibrio campbellii strain DS40M4

42.995

100

0.435
  pilC Vibrio cholerae strain A1552

40.931

99.756

0.408
  pilC Thermus thermophilus HB27

39.13

100

0.396