Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   N288_RS06730 Genome accession   NC_022524
Coordinates   1299317..1303081 (+) Length   1254 a.a.
NCBI ID   WP_009794015.1    Uniprot ID   U5L6B6
Organism   Bacillus infantis NRRL B-14911     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1294317..1308081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N288_RS06720 (N288_07205) - 1294340..1295467 (-) 1128 WP_009794013.1 MFS transporter -
  N288_RS06725 (N288_07210) addB 1295833..1299324 (+) 3492 WP_009794014.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  N288_RS06730 (N288_07215) addA 1299317..1303081 (+) 3765 WP_009794015.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  N288_RS06735 (N288_07220) - 1303159..1303461 (+) 303 WP_009794016.1 HNH endonuclease -
  N288_RS06740 (N288_07225) - 1303506..1303724 (-) 219 WP_009794017.1 spore germination protein -
  N288_RS06745 (N288_07230) - 1303769..1304146 (-) 378 WP_009794018.1 spore germination protein GerPE -
  N288_RS06750 (N288_07235) - 1304151..1304327 (-) 177 WP_009794019.1 hypothetical protein -
  N288_RS06755 (N288_07240) - 1304324..1304941 (-) 618 WP_022543592.1 spore germination protein GerPC -
  N288_RS06760 (N288_07245) - 1304978..1305208 (-) 231 WP_009794021.1 spore germination protein GerPB -
  N288_RS06765 (N288_07250) - 1305223..1305444 (-) 222 WP_009794022.1 spore germination protein -
  N288_RS06770 (N288_07255) - 1305608..1307200 (+) 1593 WP_022543593.1 long-chain-fatty-acid--CoA ligase -

Sequence

Protein


Download         Length: 1254 a.a.        Molecular weight: 142667.96 Da        Isoelectric Point: 4.9411

>NTDB_id=62491 N288_RS06730 WP_009794015.1 1299317..1303081(+) (addA) [Bacillus infantis NRRL B-14911]
MAKAIIPPKPEGAAWTDDQWKAIMASGQDILVAAAAGSGKTAVLVERIINKIVSREEPVNVDELLVVTFTNASAAEMRHR
IGEALEKAINRDPQSAHLRKQLSLLSRASISTLHSFCLEVIRKYYYLIDIDPGFRIADETEGQLLRDEVLEELFEEEYGK
ENNEDFYRLVDAFTSDRSDTALQDIIRELYDFARSNPAPDQYLDKISDMYEVSSDAKLEELPFMSSVLFDIGLQLEGARQ
LLENALELAKTPGGPAPRAENYMDDIRAVETLIAANNDSWSSLNEAMQLWSFGRAKNCKGDEYDPDLVKQAQKYRDKAKK
MIQDLQAEFFSRRPESFIRDIGEMKPHIDVLVRLVRSFSEKFNSVKSEKGLVDFADLEHFCLEILSDPSARKEGAILPSE
PALTYRDHFREVLVDEYQDTNMVQEAILQLVTKEGEETGNLFMVGDVKQSIYRFRLAEPNLFLGKYTRFTVDGEKTGLKI
DLARNFRSRKEVLDGTNFLFKQVMGKKVGEIDYDQAAELVKGASYPEDLPYPVELHLIDLDGQEKAESGEADETDAGYDL
EDLEQSQHEARLMAEKIKEMLSSGKKVYEPKSGAYRPLMYRDIVILLRSMTWAPQIMEEFKKQGIPIYANLSSGYFEATE
ITIMMSLLKVIDNPHQDIPLASVLRSPIVGLNEEELAQIRIADKKGSYYEAMKAFCETKKSGDLYDKVKPFFLDIAEWRS
MARKGGLSELIWQLYRDTHFYDFAGGMPGGKQRQANLRALYDRARQYEETSFRGLFRFLRFIERMRERGDDLGAARALGE
QEDVVRMMTIHSSKGLEFPVVFAAGLARQFNTMDLKKTYLLDKEYGFAAKYVNPEKRISYPSLPQLAFKRKKKMEMLAEE
MRVLYVALTRAKEHLFLVASVKNADKKLAGWQQGAKKDSWLLDEYDRAAASSYLDWIGSALIRHRDGEPLRSGQEDAAAG
SQGEIALHPSSWSVEVRQAAVTPAQDEDSDEAGEQWIDSVYEGKPVPAESPFKEKIKNSLSWQYSYPSAASHKSKQSVSE
MKRQSEVKDEESGSDLVRNLKKPILNRPRFMQEKSMTPAERGTAMHMVMQHADLSNPVTEESIERQLAEMKDRELLTDEQ
VQVIDPALIAGFFQSPLGQRMRKAARIQREVPFNLALPARTAYPDWKGTEEPVLVQGIIDCIFEDEHGTVLLDYKSDSIH
GRYTGGFEEAMPVLKRRYDVQISLYAKAYEAITKDPVDERYLFFFDGGHILKLE

Nucleotide


Download         Length: 3765 bp        

>NTDB_id=62491 N288_RS06730 WP_009794015.1 1299317..1303081(+) (addA) [Bacillus infantis NRRL B-14911]
ATGGCTAAAGCTATTATTCCTCCAAAGCCGGAGGGGGCAGCCTGGACAGATGACCAGTGGAAGGCAATCATGGCTTCGGG
CCAGGATATCCTTGTGGCTGCAGCTGCAGGCTCCGGAAAAACGGCAGTGCTGGTTGAAAGGATCATCAATAAGATCGTAT
CCAGGGAAGAGCCGGTCAATGTGGATGAGCTCCTCGTCGTGACCTTTACGAATGCGTCTGCCGCAGAGATGAGGCACAGG
ATCGGGGAGGCACTTGAGAAGGCGATAAACAGGGATCCGCAGTCTGCGCATTTGCGCAAGCAGCTCAGCCTGCTGAGCAG
GGCTTCGATTTCCACTCTTCATTCATTCTGCCTTGAAGTGATCCGGAAATATTATTATCTGATCGATATCGATCCCGGCT
TCCGCATTGCCGATGAGACAGAGGGGCAGCTCCTCAGGGATGAAGTCCTTGAAGAACTGTTCGAAGAAGAATACGGCAAG
GAAAACAATGAAGACTTTTACCGGCTGGTCGATGCTTTCACGAGCGACAGGAGTGACACAGCCCTCCAGGATATCATCAG
GGAACTGTACGATTTTGCACGGTCCAATCCGGCACCAGATCAATATCTCGATAAAATCTCGGACATGTACGAAGTCAGCA
GTGATGCAAAACTGGAAGAGCTTCCGTTCATGTCAAGCGTCTTGTTTGATATCGGACTGCAGCTTGAGGGAGCAAGGCAG
CTGCTGGAAAATGCGCTTGAGCTTGCCAAAACGCCTGGCGGGCCTGCTCCAAGGGCAGAGAATTATATGGACGATATCAG
GGCAGTTGAGACCTTGATTGCCGCAAACAATGATTCCTGGTCCAGTTTAAATGAAGCCATGCAGCTTTGGAGCTTTGGCC
GTGCGAAAAATTGCAAAGGCGATGAATATGATCCCGATCTGGTCAAGCAGGCCCAAAAGTATAGAGATAAGGCAAAGAAA
ATGATCCAGGATCTGCAGGCGGAATTCTTTTCGCGCAGGCCGGAAAGCTTCATCCGGGATATTGGGGAGATGAAGCCGCA
TATCGATGTACTCGTCCGGCTCGTCCGGTCCTTTTCAGAGAAATTCAACAGTGTGAAAAGTGAGAAGGGACTTGTTGACT
TTGCTGATCTGGAGCATTTTTGCCTGGAAATCCTTTCTGATCCTTCTGCAAGGAAGGAGGGAGCCATCCTGCCTTCAGAG
CCAGCTCTTACTTACCGGGACCATTTCAGGGAAGTGCTTGTTGATGAATACCAGGACACCAACATGGTCCAGGAAGCAAT
CCTGCAGCTCGTGACGAAGGAGGGGGAAGAGACTGGCAATCTGTTTATGGTTGGAGACGTGAAGCAATCCATTTACCGGT
TCAGGCTGGCAGAACCCAATCTGTTCCTCGGCAAATATACTCGCTTTACTGTGGACGGAGAGAAAACAGGATTAAAGATC
GACCTTGCAAGAAACTTCCGCAGCAGAAAGGAAGTGCTTGACGGGACCAACTTCCTTTTCAAACAGGTGATGGGAAAAAA
AGTAGGGGAAATCGATTATGACCAGGCTGCCGAGCTTGTAAAGGGAGCTTCGTATCCTGAAGACCTTCCGTATCCTGTTG
AGCTTCATCTGATCGACCTGGACGGACAGGAGAAGGCCGAAAGCGGGGAAGCAGATGAAACGGATGCAGGGTATGACCTT
GAGGATCTCGAGCAATCACAGCATGAAGCAAGGCTGATGGCTGAGAAAATCAAAGAAATGCTGTCATCCGGGAAAAAAGT
GTATGAACCGAAATCGGGAGCATATCGTCCTTTAATGTACCGCGATATTGTCATCCTTCTAAGGTCGATGACATGGGCTC
CGCAGATTATGGAGGAATTCAAAAAGCAGGGAATTCCGATTTATGCAAATCTGTCTTCAGGCTATTTTGAAGCTACCGAG
ATCACCATTATGATGAGCCTTTTAAAAGTGATAGACAATCCGCATCAGGATATCCCGCTTGCCTCTGTTTTGCGGTCTCC
GATAGTCGGCCTTAACGAAGAAGAGCTTGCCCAGATCCGCATCGCCGATAAAAAGGGTTCCTACTATGAAGCGATGAAGG
CATTCTGTGAAACAAAGAAAAGCGGGGATCTGTATGATAAAGTAAAGCCGTTTTTCCTGGATATTGCGGAATGGAGATCG
ATGGCGAGGAAAGGCGGTCTTTCTGAATTGATCTGGCAGCTGTACCGGGACACGCATTTCTATGACTTTGCCGGAGGGAT
GCCCGGCGGCAAGCAAAGACAGGCTAATCTCCGGGCACTGTATGACCGGGCACGCCAATATGAGGAAACTTCCTTCAGGG
GCCTTTTCCGCTTCCTGCGTTTCATTGAACGGATGAGGGAACGGGGCGATGACCTTGGTGCAGCGCGCGCACTCGGCGAA
CAGGAGGATGTGGTGAGGATGATGACCATCCACAGCAGCAAGGGGCTTGAATTTCCTGTCGTCTTTGCTGCAGGCCTGGC
AAGGCAGTTCAATACGATGGATTTGAAAAAAACCTATCTGCTTGATAAGGAATACGGATTTGCGGCCAAATATGTGAATC
CGGAGAAGCGGATTTCCTATCCATCCCTGCCACAGCTTGCTTTTAAAAGGAAAAAGAAGATGGAAATGCTGGCAGAGGAA
ATGCGCGTTCTGTATGTAGCACTGACTCGTGCCAAAGAACATCTTTTTCTTGTGGCAAGCGTGAAAAATGCGGACAAAAA
ACTGGCCGGCTGGCAGCAGGGTGCCAAAAAAGACAGCTGGCTGCTGGATGAATATGACCGGGCGGCTGCTTCCAGCTATC
TTGATTGGATCGGCTCCGCACTTATCCGCCACCGGGACGGGGAGCCACTGAGAAGCGGGCAGGAGGATGCCGCAGCAGGA
AGCCAGGGAGAAATCGCTTTACATCCTTCATCCTGGAGCGTTGAGGTCAGGCAGGCAGCCGTCACTCCTGCTCAGGATGA
GGACAGTGATGAAGCCGGGGAGCAGTGGATTGACAGCGTATATGAAGGCAAACCGGTCCCGGCTGAGTCCCCTTTTAAAG
AAAAAATAAAGAATTCACTTTCCTGGCAGTACAGCTACCCTTCCGCAGCTTCCCATAAGTCAAAGCAATCTGTCTCAGAA
ATGAAGCGGCAGAGTGAAGTGAAGGATGAAGAGAGCGGCAGCGATCTCGTCAGGAATCTGAAAAAGCCGATCCTGAACCG
GCCAAGGTTCATGCAGGAGAAATCAATGACACCTGCCGAAAGGGGCACGGCGATGCATATGGTCATGCAGCATGCAGACC
TTTCCAATCCGGTGACAGAAGAGTCCATTGAGAGACAACTGGCGGAGATGAAAGATCGGGAGCTTCTCACGGATGAGCAA
GTCCAGGTGATAGACCCGGCCCTCATCGCGGGATTCTTCCAATCGCCTCTTGGCCAGAGGATGAGAAAGGCTGCAAGGAT
CCAGCGGGAGGTTCCTTTCAACCTGGCATTGCCGGCAAGAACCGCCTACCCGGACTGGAAGGGCACAGAAGAACCGGTGC
TTGTCCAGGGGATCATTGACTGCATTTTCGAGGATGAGCACGGTACAGTCCTGCTTGATTATAAGTCTGACAGCATCCAT
GGGCGCTACACGGGCGGATTTGAGGAGGCAATGCCGGTCCTCAAAAGACGGTATGATGTTCAGATAAGCCTGTATGCAAA
AGCTTATGAGGCTATTACTAAGGACCCTGTTGATGAAAGGTATCTATTCTTTTTTGACGGAGGCCATATTTTAAAGCTGG
AATAG

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(480-899)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(15-469)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1073-1243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U5L6B6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

55.885

99.601

0.557

Multiple sequence alignment