Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   LMK00_RS02030 Genome accession   NZ_CP086395
Coordinates   421710..422351 (+) Length   213 a.a.
NCBI ID   WP_165711419.1    Uniprot ID   -
Organism   Lactococcus formosensis strain I4/6O     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416710..427351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMK00_RS02015 (LMK00_02015) sufB 417060..418457 (+) 1398 WP_165711421.1 Fe-S cluster assembly protein SufB -
  LMK00_RS02020 (LMK00_02020) - 418756..420312 (+) 1557 WP_252175605.1 ABC-F family ATP-binding cassette domain-containing protein -
  LMK00_RS02025 (LMK00_02025) - 420477..421652 (+) 1176 WP_096368625.1 SLC13 family permease -
  LMK00_RS02030 (LMK00_02030) comEA/celA/cilE 421710..422351 (+) 642 WP_165711419.1 helix-hairpin-helix domain-containing protein Machinery gene
  LMK00_RS02035 (LMK00_02035) comEC 422371..424581 (+) 2211 WP_252175606.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LMK00_RS02040 (LMK00_02040) - 424736..425512 (+) 777 WP_185767510.1 alpha/beta hydrolase family protein -
  LMK00_RS02045 (LMK00_02045) - 425708..425923 (+) 216 WP_003135074.1 F0F1 ATP synthase subunit C -
  LMK00_RS02050 (LMK00_02050) atpB 425978..426691 (+) 714 WP_252175607.1 F0F1 ATP synthase subunit A -
  LMK00_RS02055 (LMK00_02055) atpF 426705..427211 (+) 507 WP_017368854.1 F0F1 ATP synthase subunit B -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 23579.75 Da        Isoelectric Point: 5.1049

>NTDB_id=624737 LMK00_RS02030 WP_165711419.1 421710..422351(+) (comEA/celA/cilE) [Lactococcus formosensis strain I4/6O]
MKKLTEYINENLKTIVGVVLALTILGIFYWNKKVPDLPKKEEDLPEWVEGSKEKDGSEKKNTEKVDEDEIIVDVKGAVKK
PGIYKLSSKNRVSDAVASAGGFTEEAERKGINLAKKLQDEAEVYVPLRGEADTNHLLENTENTNKTAKKAKININTADLT
ELQQLSGVGAKKAQDILDYRSENGSFQTVEELTKVSGFGPKTLEKLKEAITVD

Nucleotide


Download         Length: 642 bp        

>NTDB_id=624737 LMK00_RS02030 WP_165711419.1 421710..422351(+) (comEA/celA/cilE) [Lactococcus formosensis strain I4/6O]
ATGAAAAAACTAACAGAATATATAAATGAAAATTTAAAAACTATTGTTGGAGTTGTTTTAGCATTGACTATTTTAGGTAT
ATTTTACTGGAATAAAAAAGTTCCTGATCTGCCTAAGAAAGAAGAAGATTTACCTGAGTGGGTAGAGGGAAGCAAGGAAA
AAGACGGGTCAGAGAAGAAAAATACTGAAAAGGTAGATGAGGATGAAATCATTGTTGATGTTAAAGGCGCTGTTAAAAAA
CCTGGTATCTACAAATTGTCTTCAAAAAACCGTGTATCCGATGCAGTAGCAAGTGCTGGTGGTTTTACGGAAGAAGCGGA
GAGAAAAGGAATAAATCTGGCAAAAAAGTTGCAAGATGAAGCTGAAGTTTATGTTCCTTTGCGAGGGGAAGCAGATACAA
ATCACTTGTTAGAAAACACAGAAAATACCAACAAAACCGCCAAAAAAGCAAAAATTAATATTAATACAGCAGATTTAACT
GAATTGCAGCAACTTAGTGGTGTGGGAGCAAAAAAAGCACAAGATATACTGGATTACCGTTCTGAAAATGGTTCCTTTCA
GACAGTGGAAGAATTGACTAAGGTGAGTGGATTTGGCCCTAAAACATTAGAAAAATTGAAAGAAGCAATCACAGTAGATT
AG

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(149-211)

SLBB domain (SLBB)

(71-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus mitis SK321

47.032

100

0.484
  comEA/celA/cilE Streptococcus mitis NCTC 12261

45.662

100

0.469
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

44.749

100

0.46
  comEA/celA/cilE Streptococcus pneumoniae Rx1

43.836

100

0.451
  comEA/celA/cilE Streptococcus pneumoniae D39

43.836

100

0.451
  comEA/celA/cilE Streptococcus pneumoniae R6

43.836

100

0.451
  comEA Lactococcus lactis subsp. cremoris KW2

43.243

100

0.451
  comEA Latilactobacillus sakei subsp. sakei 23K

36.937

100

0.385
  comEA Staphylococcus aureus MW2

35.616

100

0.366