Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   LMK00_RS02035 Genome accession   NZ_CP086395
Coordinates   422371..424581 (+) Length   736 a.a.
NCBI ID   WP_252175606.1    Uniprot ID   A0A9Q8Y2X0
Organism   Lactococcus formosensis strain I4/6O     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417371..429581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMK00_RS02020 (LMK00_02020) - 418756..420312 (+) 1557 WP_252175605.1 ABC-F family ATP-binding cassette domain-containing protein -
  LMK00_RS02025 (LMK00_02025) - 420477..421652 (+) 1176 WP_096368625.1 SLC13 family permease -
  LMK00_RS02030 (LMK00_02030) comEA/celA/cilE 421710..422351 (+) 642 WP_165711419.1 helix-hairpin-helix domain-containing protein Machinery gene
  LMK00_RS02035 (LMK00_02035) comEC 422371..424581 (+) 2211 WP_252175606.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LMK00_RS02040 (LMK00_02040) - 424736..425512 (+) 777 WP_185767510.1 alpha/beta hydrolase family protein -
  LMK00_RS02045 (LMK00_02045) - 425708..425923 (+) 216 WP_003135074.1 F0F1 ATP synthase subunit C -
  LMK00_RS02050 (LMK00_02050) atpB 425978..426691 (+) 714 WP_252175607.1 F0F1 ATP synthase subunit A -
  LMK00_RS02055 (LMK00_02055) atpF 426705..427211 (+) 507 WP_017368854.1 F0F1 ATP synthase subunit B -
  LMK00_RS02060 (LMK00_02060) - 427226..427753 (+) 528 WP_017368855.1 F0F1 ATP synthase subunit delta -
  LMK00_RS02065 (LMK00_02065) atpA 427819..429327 (+) 1509 WP_004259170.1 F0F1 ATP synthase subunit alpha -

Sequence

Protein


Download         Length: 736 a.a.        Molecular weight: 84712.73 Da        Isoelectric Point: 9.9010

>NTDB_id=624738 LMK00_RS02035 WP_252175606.1 422371..424581(+) (comEC) [Lactococcus formosensis strain I4/6O]
MKQKFSLIYLVYLLVLVYFLIFSFSWPIIVFSLFSIIVALYRQYYGLLALLLCFSLFLHMVKKEDELQENKQPAKISKIT
PYMDTLQVNGSRLSFRGEVDGRDYQAFYTLSSEKEKEYFKNLAVKGTLYLEGTLEIPEGQRNFTGFDYRKYLRTQGIYRL
LEIEKIHGFEQTYEFDLRLIRRRAILWTQEHFPAPMSSYMTGLLFGWLDKDFEEMEELYTSLGIIHLFALSGMQVNFFIE
LLRNVLLRLGLSRKLVFFIQIPFSIFYAFLTGLSLSILRALLQKNIPFKNPSDRFSITFLLLIVFSPYFLLTTGGQLTML
YSSLITFISRKFKEVKGLKKSLLDSTVLAVGVLPLLVFHFHSFQPLSIILTLLFSMIFDFLLLPGLLFVFLLSLLGINLT
LLNPFFVVLESVVKLVGNLFDKPIVFGKPDVIFLLLLFILSGLILDFYKKRNWNLVIILVLFCIFFLVKNPKHPSITMVD
VGQGDSILLQDSWNSKNILIDTGGRLKIRTDEHWREGSYKSNAERTLLPYLRARGVSKIDALIVTHPDEDHIGDLKSVAQ
KVPIKNIYVSASALEKQIFVKKLSSLKRQIHIVQEGDKIPIFNSYLRFLTTGGEGKSDNDNSLVTFGEFYGKKFLFTGDL
EEEGEAKLQKGYPHLEVDILKVGHHGSKTSTKEGFLEAIKPKIALISAGKNNRYKHPSQETLHQLTARGIKIYRTDQQGA
IRLLYKKGSWHIQTVK

Nucleotide


Download         Length: 2211 bp        

>NTDB_id=624738 LMK00_RS02035 WP_252175606.1 422371..424581(+) (comEC) [Lactococcus formosensis strain I4/6O]
ATGAAACAAAAATTTTCGCTTATCTATTTAGTTTATCTCTTGGTTTTGGTTTATTTTCTTATTTTTAGTTTTTCGTGGCC
TATTATTGTTTTTAGCCTTTTCAGTATTATTGTTGCTTTATATCGTCAATATTATGGTTTGTTAGCTCTTTTACTATGCT
TTAGCTTGTTCTTACACATGGTTAAAAAAGAAGATGAATTGCAAGAAAATAAACAACCAGCAAAGATTTCTAAAATCACA
CCCTACATGGATACTCTTCAAGTGAATGGAAGTAGGCTCAGTTTTAGAGGTGAAGTAGATGGAAGAGATTATCAAGCTTT
TTACACGTTGTCTTCTGAAAAAGAGAAAGAATACTTTAAAAATCTTGCCGTAAAGGGGACATTATATCTTGAAGGTACTT
TGGAGATTCCAGAGGGACAGCGAAACTTTACTGGCTTTGATTATAGAAAGTATTTAAGAACTCAAGGTATTTATCGACTT
CTCGAAATAGAAAAAATTCATGGCTTTGAACAAACATATGAATTTGATTTACGTCTTATACGTAGAAGAGCTATCCTTTG
GACACAGGAACATTTTCCAGCACCGATGTCCTCCTATATGACAGGACTTCTGTTTGGTTGGCTAGATAAAGACTTTGAAG
AAATGGAGGAGCTCTACACTAGTCTCGGTATTATCCATTTATTTGCTTTGTCAGGTATGCAAGTGAATTTTTTTATTGAA
CTCCTTCGAAATGTTTTACTAAGACTAGGCTTATCACGAAAGTTAGTCTTTTTTATCCAGATTCCCTTTTCGATATTTTA
TGCATTTCTTACAGGCTTATCTCTTTCAATCTTACGTGCGCTTCTACAAAAAAATATCCCTTTTAAAAATCCAAGCGATA
GATTTTCTATCACTTTTCTTCTTTTAATAGTGTTCAGTCCATATTTTCTACTGACTACAGGGGGACAATTGACGATGCTC
TATTCTTCCTTAATCACGTTCATATCGCGAAAATTCAAAGAAGTGAAAGGATTAAAAAAGTCTTTACTTGATTCTACTGT
TTTAGCTGTAGGAGTCCTTCCGCTTTTGGTTTTTCATTTCCATAGTTTTCAGCCATTATCGATAATATTGACTTTACTAT
TCAGTATGATCTTTGATTTTTTACTCTTACCTGGTTTGCTTTTTGTTTTTCTCCTTTCTTTACTTGGTATTAATCTGACA
TTGCTTAATCCTTTTTTTGTTGTTTTAGAATCAGTAGTAAAGCTGGTTGGGAATTTATTCGACAAACCAATTGTGTTTGG
AAAACCAGATGTTATATTTTTACTTTTACTTTTTATTCTGAGTGGACTTATACTGGATTTTTATAAAAAAAGAAATTGGA
ATTTAGTCATAATACTTGTTCTTTTCTGTATTTTTTTTCTGGTTAAGAATCCTAAACACCCAAGTATTACAATGGTGGAT
GTGGGACAAGGAGACAGTATTCTCTTGCAGGACAGCTGGAACAGTAAAAATATCCTAATAGATACTGGCGGTAGATTAAA
GATAAGAACTGATGAGCACTGGAGGGAAGGAAGCTATAAAAGTAATGCGGAGCGTACACTTCTCCCTTATCTGAGAGCGC
GTGGAGTGAGTAAGATAGATGCCCTGATAGTGACACATCCAGATGAGGATCATATTGGAGACTTGAAGAGTGTAGCTCAG
AAAGTACCAATAAAAAATATTTATGTCTCAGCTAGTGCATTAGAGAAGCAAATTTTTGTGAAAAAACTAAGCAGCTTAAA
ACGTCAGATTCACATTGTGCAAGAGGGAGACAAGATTCCCATATTTAACAGCTATCTCCGATTTTTAACGACAGGAGGTG
AAGGAAAAAGCGATAATGATAATTCTTTAGTGACATTTGGTGAATTTTATGGGAAGAAATTTCTTTTTACAGGTGACTTA
GAAGAAGAGGGAGAGGCTAAACTCCAGAAAGGATATCCACATTTAGAGGTTGATATCCTAAAAGTTGGACATCACGGCAG
TAAGACTTCTACAAAGGAAGGTTTTTTAGAAGCAATTAAGCCTAAGATTGCTCTTATTTCTGCTGGAAAAAATAATCGAT
ATAAACATCCGAGTCAAGAGACGCTTCACCAATTAACTGCACGAGGAATAAAAATTTATCGGACAGACCAGCAAGGAGCG
ATCCGTCTCCTATATAAAAAAGGCAGCTGGCACATTCAAACAGTAAAGTAA

Domains

Predicted by InterproScan.

Metallo-beta-lactamase superfamily (Lactamase_B)

(481-689)

Competence protein (Competence)

(203-445)

Domain of unknown function (DUF4131) (DUF4131)

(21-166)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Lactococcus lactis subsp. cremoris KW2

51.978

99.592

0.518
  comEC/celB Streptococcus mitis SK321

45.71

100

0.463
  comEC/celB Streptococcus mitis NCTC 12261

44.295

100

0.448
  comEC/celB Streptococcus pneumoniae TIGR4

43.817

100

0.443
  comEC/celB Streptococcus pneumoniae Rx1

43.683

100

0.442
  comEC/celB Streptococcus pneumoniae D39

43.683

100

0.442
  comEC/celB Streptococcus pneumoniae R6

43.683

100

0.442