Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KM317_RS05895 Genome accession   NZ_CP086333
Coordinates   1415633..1416763 (+) Length   376 a.a.
NCBI ID   WP_009599961.1    Uniprot ID   A0A0K2ZME5
Organism   Xanthomonas translucens pv. arrhenatheri strain LMG 727     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1410633..1421763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM317_RS05870 (KM317_05870) - 1410872..1411327 (-) 456 WP_053834938.1 DUF4426 domain-containing protein -
  KM317_RS05875 (KM317_05875) proC 1411342..1412184 (-) 843 WP_053834937.1 pyrroline-5-carboxylate reductase -
  KM317_RS05880 (KM317_05880) - 1412335..1413363 (-) 1029 WP_053834994.1 hypothetical protein -
  KM317_RS05885 (KM317_05885) - 1413661..1414311 (-) 651 WP_053834936.1 YggS family pyridoxal phosphate-dependent enzyme -
  KM317_RS05890 (KM317_05890) pilT 1414435..1415472 (+) 1038 WP_003471666.1 type IV pilus twitching motility protein PilT Machinery gene
  KM317_RS05895 (KM317_05895) pilU 1415633..1416763 (+) 1131 WP_009599961.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KM317_RS05900 (KM317_05900) - 1417018..1417557 (-) 540 WP_053834935.1 DNA-3-methyladenine glycosylase I -
  KM317_RS05905 (KM317_05905) - 1417573..1419381 (-) 1809 WP_053834993.1 DUF4153 domain-containing protein -
  KM317_RS05910 (KM317_05910) - 1419489..1420055 (+) 567 WP_053834934.1 YqgE/AlgH family protein -
  KM317_RS05915 (KM317_05915) ruvX 1420048..1420545 (+) 498 WP_053834933.1 Holliday junction resolvase RuvX -
  KM317_RS05920 (KM317_05920) - 1420565..1421512 (+) 948 WP_053834932.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41958.30 Da        Isoelectric Point: 6.8811

>NTDB_id=624353 KM317_RS05895 WP_009599961.1 1415633..1416763(+) (pilU) [Xanthomonas translucens pv. arrhenatheri strain LMG 727]
MSTIDFTSFLKLMAHQKASDLFITAGMPPAIKVHGKITPITQTPLTAQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNMRGVVAQQLIPTPDGKSRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=624353 KM317_RS05895 WP_009599961.1 1415633..1416763(+) (pilU) [Xanthomonas translucens pv. arrhenatheri strain LMG 727]
ATGAGCACCATCGACTTCACCTCATTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCGCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCGCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCTGGG
GTCGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGTATCCC
CACCGTCGACGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCGATCTCCTTCGCCGAGACCGGCCACCTGGTGTTGTGCACCCTGCATGCCAACAACGCCAATCAGGCGATG
GACCGGATCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACTTGTCGCTGAACATGCGCGGCGTGGT
CGCCCAGCAGCTGATCCCGACCCCCGACGGCAAGAGCCGCCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAATCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCCACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCAGGACCCTGGCGCAGGGCATGGACGGGGTGGAAATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K2ZME5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.583

98.138

0.644
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.571

93.085

0.396
  pilT Pseudomonas aeruginosa PAK

40.708

90.16

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367