Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M5R25_RS20050 Genome accession   NZ_CP103657
Coordinates   4152389..4153774 (+) Length   461 a.a.
NCBI ID   WP_001025174.1    Uniprot ID   -
Organism   Escherichia coli strain 3150     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4147389..4158774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5R25_RS20025 (M5R25_20025) aroP 4147937..4149310 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  M5R25_RS20030 (M5R25_20030) ampE 4149353..4150207 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  M5R25_RS20035 (M5R25_20035) ampD 4150204..4150755 (-) 552 WP_022645179.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M5R25_RS20040 (M5R25_20040) nadC 4150843..4151736 (+) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M5R25_RS20045 (M5R25_20045) pilA 4151939..4152379 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  M5R25_RS20050 (M5R25_20050) pilB 4152389..4153774 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  M5R25_RS20055 (M5R25_20055) hofC 4153764..4154966 (+) 1203 WP_000157236.1 protein transport protein HofC -
  M5R25_RS20060 (M5R25_20060) guaC 4155001..4156044 (-) 1044 WP_001217338.1 GMP reductase -
  M5R25_RS20065 (M5R25_20065) - 4156200..4156244 (-) 45 WP_120795372.1 protein YacM -
  M5R25_RS20070 (M5R25_20070) coaE 4156269..4156889 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  M5R25_RS20075 (M5R25_20075) zapD 4156889..4157632 (+) 744 WP_001194734.1 cell division protein ZapD -
  M5R25_RS20080 (M5R25_20080) yacG 4157642..4157839 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  M5R25_RS20085 (M5R25_20085) mutT 4157939..4158328 (-) 390 WP_000736013.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50480.22 Da        Isoelectric Point: 6.5385

>NTDB_id=624074 M5R25_RS20050 WP_001025174.1 4152389..4153774(+) (pilB) [Escherichia coli strain 3150]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLARTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=624074 M5R25_RS20050 WP_001025174.1 4152389..4153774(+) (pilB) [Escherichia coli strain 3150]
ATGAATATTCCACAGCTCACGGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAGCTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGTGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Legionella pneumophila strain ERS1305867

49.738

82.863

0.412
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.062

83.948

0.403
  pilB Vibrio cholerae strain A1552

46.465

85.9

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.373

83.731

0.388
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.655

85.466

0.373
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilF Thermus thermophilus HB27

40.587

88.72

0.36