Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M5S90_RS20115 Genome accession   NZ_CP103633
Coordinates   4188331..4189716 (+) Length   461 a.a.
NCBI ID   WP_001025167.1    Uniprot ID   -
Organism   Escherichia coli strain 2900     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4183331..4194716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5S90_RS20090 (M5S90_20090) aroP 4183879..4185252 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  M5S90_RS20095 (M5S90_20095) ampE 4185295..4186149 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  M5S90_RS20100 (M5S90_20100) ampD 4186146..4186697 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M5S90_RS20105 (M5S90_20105) nadC 4186785..4187678 (+) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M5S90_RS20110 (M5S90_20110) pilA 4187881..4188321 (+) 441 WP_020233150.1 prepilin peptidase-dependent pilin Machinery gene
  M5S90_RS20115 (M5S90_20115) pilB 4188331..4189716 (+) 1386 WP_001025167.1 type II secretion system protein GspE Machinery gene
  M5S90_RS20120 (M5S90_20120) hofC 4189706..4190908 (+) 1203 WP_021522995.1 protein transport protein HofC -
  M5S90_RS20125 (M5S90_20125) guaC 4190943..4191986 (-) 1044 WP_001217337.1 GMP reductase -
  M5S90_RS20130 (M5S90_20130) - 4192142..4192186 (-) 45 WP_120795372.1 protein YacM -
  M5S90_RS20135 (M5S90_20135) coaE 4192211..4192831 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  M5S90_RS20140 (M5S90_20140) zapD 4192831..4193574 (+) 744 WP_001194736.1 cell division protein ZapD -
  M5S90_RS20145 (M5S90_20145) yacG 4193584..4193781 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  M5S90_RS20150 (M5S90_20150) mutT 4193983..4194381 (-) 399 WP_000736024.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50655.35 Da        Isoelectric Point: 6.6256

>NTDB_id=623904 M5S90_RS20115 WP_001025167.1 4188331..4189716(+) (pilB) [Escherichia coli strain 2900]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRTALFE
VLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=623904 M5S90_RS20115 WP_001025167.1 4188331..4189716(+) (pilB) [Escherichia coli strain 2900]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTATTCATGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATACGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGGAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCCCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCGCTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTACCCATAACACCGATCATACGCCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACACACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.469

100

0.416
  pilB Legionella pneumophila strain ERS1305867

49.74

83.297

0.414
  pilB Haemophilus influenzae 86-028NP

39.915

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.187

83.731

0.403
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Vibrio cholerae strain A1552

46.41

84.599

0.393
  pilB Acinetobacter baylyi ADP1

45.22

83.948

0.38
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilB Haemophilus influenzae Rd KW20

43.928

83.948

0.369
  pilB Acinetobacter baumannii D1279779

43.864

83.08

0.364
  pilF Thermus thermophilus HB27

41.032

88.286

0.362