Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M5S90_RS20110 Genome accession   NZ_CP103633
Coordinates   4187881..4188321 (+) Length   146 a.a.
NCBI ID   WP_020233150.1    Uniprot ID   -
Organism   Escherichia coli strain 2900     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4182881..4193321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5S90_RS20090 (M5S90_20090) aroP 4183879..4185252 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  M5S90_RS20095 (M5S90_20095) ampE 4185295..4186149 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  M5S90_RS20100 (M5S90_20100) ampD 4186146..4186697 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M5S90_RS20105 (M5S90_20105) nadC 4186785..4187678 (+) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M5S90_RS20110 (M5S90_20110) pilA 4187881..4188321 (+) 441 WP_020233150.1 prepilin peptidase-dependent pilin Machinery gene
  M5S90_RS20115 (M5S90_20115) pilB 4188331..4189716 (+) 1386 WP_001025167.1 type II secretion system protein GspE Machinery gene
  M5S90_RS20120 (M5S90_20120) hofC 4189706..4190908 (+) 1203 WP_021522995.1 protein transport protein HofC -
  M5S90_RS20125 (M5S90_20125) guaC 4190943..4191986 (-) 1044 WP_001217337.1 GMP reductase -
  M5S90_RS20130 (M5S90_20130) - 4192142..4192186 (-) 45 WP_120795372.1 protein YacM -
  M5S90_RS20135 (M5S90_20135) coaE 4192211..4192831 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=623903 M5S90_RS20110 WP_020233150.1 4187881..4188321(+) (pilA) [Escherichia coli strain 2900]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTNMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=623903 M5S90_RS20110 WP_020233150.1 4187881..4188321(+) (pilA) [Escherichia coli strain 2900]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCAACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACTACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCAAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363