Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LL925_RS07435 Genome accession   NZ_CP086195
Coordinates   1545013..1546143 (+) Length   376 a.a.
NCBI ID   WP_011407963.1    Uniprot ID   A0A0K0GNA2
Organism   Xanthomonas oryzae strain JS158-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1540013..1551143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL925_RS07415 (LL925_07405) - 1540512..1541831 (-) 1320 Protein_1408 IS701 family transposase -
  LL925_RS07420 (LL925_07410) proC 1541902..1542762 (-) 861 WP_011407961.1 pyrroline-5-carboxylate reductase -
  LL925_RS07425 (LL925_07415) - 1542806..1543498 (-) 693 WP_011407962.1 YggS family pyridoxal phosphate-dependent enzyme -
  LL925_RS07430 (LL925_07420) pilT 1543862..1544899 (+) 1038 WP_011258220.1 type IV pilus twitching motility protein PilT Machinery gene
  LL925_RS07435 (LL925_07425) pilU 1545013..1546143 (+) 1131 WP_011407963.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LL925_RS07440 (LL925_07430) - 1546415..1547089 (+) 675 WP_027703695.1 YitT family protein -
  LL925_RS22895 - 1547424..1547676 (-) 253 Protein_1414 DUF72 domain-containing protein -
  LL925_RS07450 (LL925_07440) - 1547713..1548285 (-) 573 WP_011407966.1 DNA-3-methyladenine glycosylase I -
  LL925_RS07455 (LL925_07445) - 1548282..1548716 (-) 435 WP_027703694.1 hypothetical protein -
  LL925_RS07460 (LL925_07450) - 1548745..1549479 (-) 735 WP_011407968.1 hypothetical protein -
  LL925_RS07465 (LL925_07455) - 1549555..1550121 (+) 567 WP_011258225.1 YqgE/AlgH family protein -
  LL925_RS07470 (LL925_07460) ruvX 1550114..1550581 (+) 468 WP_011407969.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41968.23 Da        Isoelectric Point: 7.1973

>NTDB_id=623529 LL925_RS07435 WP_011407963.1 1545013..1546143(+) (pilU) [Xanthomonas oryzae strain JS158-2]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLSPVIKTLAMTKRGIVIFVGATGAGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPKDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGMDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=623529 LL925_RS07435 WP_011407963.1 1545013..1546143(+) (pilU) [Xanthomonas oryzae strain JS158-2]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGAATGGCAAGATCAGCCCGATTACCCAGACGCCGCTGACTGCGCAGCAGAGCCGCGATCTGG
TGTTGAACGTGATGACTCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTTTCAGGG
GTGGGGCGTTTTCGTGTGAGCTGTTTCTACCAGCGTAATCAGGTCGGTATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGTCTCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCGTCATCTTCGTCGGCG
CCACCGGTGCCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAATCAGGCGATG
GACCGCATCATCAACTTCTTCCCCAAAGACCGCCGCAATCAGCTGCTGATGGACCTGTCGCTCAATCTCAAGGGCGTGGT
GGCGCAGCAGCTGATTCCGACGCCGGATGGCCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAAATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACTTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCTGGCGAAATCAGCTACGAAGACGCATTGCGCTACGCCGACTCGCAGAACGA
AGTGCGCTTGCGCATCAAGCTCTCGCAGGGGGGCGATGCCAAGACCTTGTCGCAGGGCATGGATGGTGTGGAGATTTCCG
AAGTTCGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(83-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K0GNA2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.457

94.947

0.394
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364