Detailed information NTG0622438

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LL046_RS09085
• Genome accession: NZ_CP086088
• Coordinates: 1814743..1815390 (-)
• Length: 215 a.a.
• NCBI ID: WP_017864913.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain EIP13A
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1809743..1820390

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL046_RS09060 (LL046_09040)	atpF	1809812..1810318 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LL046_RS09065 (LL046_09045)	atpB	1810333..1811046 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LL046_RS09070 (LL046_09050)	-	1811091..1811306 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LL046_RS09075 (LL046_09055)	-	1811491..1812267 (-)	777	WP_017864911.1	alpha/beta hydrolase family protein	-
LL046_RS09080 (LL046_09060)	comEC	1812552..1814762 (-)	2211	WP_058210776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LL046_RS09085 (LL046_09065)	comEA	1814743..1815390 (-)	648	WP_017864913.1	ComEA family DNA-binding protein	Machinery gene
LL046_RS09090 (LL046_09070)	-	1815450..1816811 (-)	1362	WP_017864914.1	ABC transporter permease	-
LL046_RS09095 (LL046_09075)	-	1816808..1817740 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LL046_RS09100 (LL046_09080)	-	1817846..1818244 (-)	399	WP_023188528.1	hypothetical protein	-
LL046_RS09105 (LL046_09085)	-	1818348..1818911 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LL046_RS09110 (LL046_09090)	-	1819084..1820262 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23598.73 Da
• Isoelectric Point: 5.0013

>NTDB_id=622438 LL046_RS09085 WP_017864913.1 1814743..1815390(-) (comEA) [Lactococcus lactis subsp. lactis strain EIP13A]
MDKILEKVKEYWKMIVLVVCGLIAGGIFHVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=622438 LL046_RS09085 WP_017864913.1 1814743..1815390(-) (comEA) [Lactococcus lactis subsp. lactis strain EIP13A]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTCACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	38.696	100	0.414
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367