Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HIFGL_RS08810 Genome accession   NC_022356
Coordinates   1826939..1828333 (+) Length   464 a.a.
NCBI ID   WP_021035704.1    Uniprot ID   -
Organism   Haemophilus influenzae KR494     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1821939..1833333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIFGL_RS08785 (HifGL_001767) rsmE 1821954..1822691 (-) 738 WP_041175381.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HIFGL_RS08790 (HifGL_001768) lnt 1822741..1824270 (-) 1530 WP_021035699.1 apolipoprotein N-acyltransferase -
  HIFGL_RS08795 (HifGL_001769) corC 1824293..1825192 (-) 900 WP_021035700.1 CNNM family magnesium/cobalt transport protein CorC -
  HIFGL_RS08800 (HifGL_001771) ampD 1825824..1826378 (-) 555 WP_021035702.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HIFGL_RS08805 (HifGL_001773) pilA 1826493..1826942 (+) 450 WP_021035703.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HIFGL_RS08810 (HifGL_001774) pilB 1826939..1828333 (+) 1395 WP_021035704.1 GspE/PulE family protein Machinery gene
  HIFGL_RS08815 (HifGL_001775) pilC 1828330..1829550 (+) 1221 WP_021035705.1 type II secretion system F family protein Machinery gene
  HIFGL_RS08820 (HifGL_001776) pilD 1829547..1830239 (+) 693 WP_041175383.1 prepilin peptidase Machinery gene
  HIFGL_RS08825 (HifGL_001777) rho 1830293..1831555 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  HIFGL_RS08830 (HifGL_001778) metJ 1831803..1832120 (+) 318 WP_021035707.1 met regulon transcriptional regulator MetJ -
  HIFGL_RS08835 (HifGL_001779) cueR 1832134..1832520 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  HIFGL_RS08840 (HifGL_001780) - 1832597..1832800 (+) 204 Protein_1715 heavy-metal-associated domain-containing protein -
  HIFGL_RS08845 (HifGL_001781) - 1832878..1833084 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52922.35 Da        Isoelectric Point: 5.6301

>NTDB_id=62178 HIFGL_RS08810 WP_021035704.1 1826939..1828333(+) (pilB) [Haemophilus influenzae KR494]
MTSYALLHTQRVIAQNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
TFLRQDPDIIMLSEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCNQGYQGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVNQGMTDNKELERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=62178 HIFGL_RS08810 WP_021035704.1 1826939..1828333(+) (pilB) [Haemophilus influenzae KR494]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAATTGCTCAAAATGGCGAGGTATTTACGATCTCGCCAGATTTATG
GGAACGTAATCAGCAACAACAGTCTCTGCTCTTACGTTATTTTGCGTTACCACTCAAAGAAGAAAATAATCGTCTTTGGT
TAGGTGTCGATTCCCTTTCTAATCTTTCCGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAGACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAATTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAGCCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACACGCACTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTGGATGGCATTATTCAAAGCCAAATTAACCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
ACTTTCTTACGCCAAGATCCCGATATTATTATGCTAAGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACCGGGCATTTAGTACTCTCAACTTTACATACCAATGATGCGATATCTGCCATTTCTCGATTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAACGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCAATCAAGGTTATCAAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAATCAAGGAATGACGGATAATA
AAGAACTTGAACGTGTGCTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

95.905

100

0.959
  pilB Haemophilus influenzae Rd KW20

95.896

99.784

0.957
  pilB Glaesserella parasuis strain SC1401

56.798

98.276

0.558
  pilB Vibrio cholerae strain A1552

40.779

100

0.429
  pilB Vibrio campbellii strain DS40M4

40.286

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.418
  pilB Legionella pneumophila strain ERS1305867

38.945

100

0.414
  pilB Acinetobacter baylyi ADP1

37.702

100

0.403
  pilB Acinetobacter baumannii D1279779

38.462

100

0.399
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.752

100

0.371
  pilF Neisseria gonorrhoeae MS11

36.134

100

0.371
  pilF Thermus thermophilus HB27

35.404

100

0.369

Multiple sequence alignment