Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HIFGL_RS08805 Genome accession   NC_022356
Coordinates   1826493..1826942 (+) Length   149 a.a.
NCBI ID   WP_021035703.1    Uniprot ID   -
Organism   Haemophilus influenzae KR494     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1821493..1831942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIFGL_RS08785 (HifGL_001767) rsmE 1821954..1822691 (-) 738 WP_041175381.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HIFGL_RS08790 (HifGL_001768) lnt 1822741..1824270 (-) 1530 WP_021035699.1 apolipoprotein N-acyltransferase -
  HIFGL_RS08795 (HifGL_001769) corC 1824293..1825192 (-) 900 WP_021035700.1 CNNM family magnesium/cobalt transport protein CorC -
  HIFGL_RS08800 (HifGL_001771) ampD 1825824..1826378 (-) 555 WP_021035702.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HIFGL_RS08805 (HifGL_001773) pilA 1826493..1826942 (+) 450 WP_021035703.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HIFGL_RS08810 (HifGL_001774) pilB 1826939..1828333 (+) 1395 WP_021035704.1 GspE/PulE family protein Machinery gene
  HIFGL_RS08815 (HifGL_001775) pilC 1828330..1829550 (+) 1221 WP_021035705.1 type II secretion system F family protein Machinery gene
  HIFGL_RS08820 (HifGL_001776) pilD 1829547..1830239 (+) 693 WP_041175383.1 prepilin peptidase Machinery gene
  HIFGL_RS08825 (HifGL_001777) rho 1830293..1831555 (-) 1263 WP_005648966.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15478.84 Da        Isoelectric Point: 8.4623

>NTDB_id=62177 HIFGL_RS08805 WP_021035703.1 1826493..1826942(+) (pilA) [Haemophilus influenzae KR494]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTSCTGGKNGIA
ADIKTAKGYVASVITQSGGITVKGNGTLANIEYILQATGNAAAGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=62177 HIFGL_RS08805 WP_021035703.1 1826493..1826942(+) (pilA) [Haemophilus influenzae KR494]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGTTTTACATTAATCGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACCATCGCAATTCCTTCTTATCAAAATTATACCAAAAAAGCTGCGGTATCCGAATTACTGCAAGCGTCTGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAACAACAAGCTGTACAGGGGGAAAAAATGGTATTGCA
GCGGATATAAAGACAGCAAAAGGCTATGTAGCCTCAGTTATCACTCAATCAGGTGGCATTACAGTAAAAGGGAATGGCAC
ATTGGCAAATATAGAATATATTTTGCAAGCTACAGGTAATGCTGCAGCAGGTGTAACTTGGACAACAACCTGCAAAGGAA
CGGATGCCTCTTTATTTCCTGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

97.315

100

0.973
  pilA Haemophilus influenzae 86-028NP

93.289

100

0.933
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila str. Paris

41.727

93.289

0.389
  pilA Vibrio campbellii strain DS40M4

40.426

94.631

0.383

Multiple sequence alignment