Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N646_RS07795 Genome accession   NC_022349
Coordinates   1734544..1736229 (+) Length   561 a.a.
NCBI ID   WP_017820394.1    Uniprot ID   -
Organism   Vibrio alginolyticus NBRC 15630 = ATCC 17749     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1729544..1741229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N646_RS07775 (N646_1613) pdhR 1731128..1731895 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  N646_RS07780 (N646_1614) ampD 1732301..1732852 (-) 552 WP_005386614.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N646_RS07785 (N646_1615) nadC 1732945..1733832 (+) 888 WP_017820396.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N646_RS07790 (N646_1616) - 1734094..1734531 (+) 438 WP_017820395.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  N646_RS07795 (N646_1617) pilB 1734544..1736229 (+) 1686 WP_017820394.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N646_RS07800 (N646_1618) pilC 1736253..1737476 (+) 1224 WP_017820393.1 type II secretion system F family protein Machinery gene
  N646_RS07805 (N646_1619) pilD 1737541..1738410 (+) 870 WP_017635919.1 A24 family peptidase Machinery gene
  N646_RS07810 (N646_1620) coaE 1738411..1739025 (+) 615 WP_017635920.1 dephospho-CoA kinase -
  N646_RS07815 (N646_1621) zapD 1739053..1739793 (+) 741 WP_017820392.1 cell division protein ZapD -
  N646_RS07820 (N646_1622) yacG 1739952..1740146 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  N646_RS07825 (N646_1623) rplS 1740611..1740964 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62225.10 Da        Isoelectric Point: 5.8463

>NTDB_id=62025 N646_RS07795 WP_017820394.1 1734544..1736229(+) (pilB) [Vibrio alginolyticus NBRC 15630 = ATCC 17749]
MLTKLSTVLRQKGLLTFSQEESLIEQVKASGISMPEALLSSGFFTSSELADHLSSLFGLNQSELSQYEYASLCQQLGLRE
LITRHNALPLQRTSSTLLLAVADPTNQQAEDDFRFATGLQVELVLADFRQLSAAIRRLYGRSLNHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKKSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=62025 N646_RS07795 WP_017820394.1 1734544..1736229(+) (pilB) [Vibrio alginolyticus NBRC 15630 = ATCC 17749]
ATGCTAACCAAACTCTCAACGGTTCTTCGCCAAAAAGGGTTACTAACATTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCGGAAGCTTTACTCAGTTCTGGATTCTTCACGTCAAGTGAGCTCGCTGATCACTTAA
GTTCTCTCTTTGGTTTAAATCAGTCTGAGTTATCTCAGTATGAGTACGCTTCGCTTTGTCAGCAACTTGGTCTTAGGGAA
TTAATCACACGGCATAACGCACTCCCACTCCAGCGTACGTCTTCAACGTTATTACTCGCCGTTGCCGACCCCACCAACCA
ACAAGCCGAAGATGACTTCCGCTTTGCCACTGGGTTACAGGTTGAACTAGTCTTGGCGGATTTTCGTCAACTCAGCGCGG
CAATTCGACGCTTGTATGGTCGTTCATTGAATCATGAGAAGTCTGGGTTAAAAGAGATCAACCAAGAAGAGTTAGCCAAC
CTGGTTGATGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATCAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGCGTGCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAACTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTACCGACTCTATTCGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAC
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACAGGGAGCGGTAAAACCGTTTCTCTATACACTGGGCTAAGTATCCTCAATAAGCCAGAAATCAA
CATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCGGATGTGGTAATGGTCGGTGAGATTCGTGACTTAGATACC
GCCGAAATCGCTATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTACATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCCTCTCTTAGTTTGATCATTGCCCAGCGCTTGGCCC
GTAAACTGTGCTCGCACTGTAAAAAATCACAAGAGCTTACCGTTCAGCTACAACATCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGTAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGCGCCTCGGTGCATGAACTAGAAAAACTCGCCATCGCAAACGGGA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

95.187

100

0.952
  pilB Vibrio campbellii strain DS40M4

90.196

100

0.902
  pilB Vibrio cholerae strain A1552

74.199

100

0.743
  pilB Acinetobacter baumannii D1279779

51.036

94.652

0.483
  pilB Legionella pneumophila strain ERS1305867

49.353

96.435

0.476
  pilB Acinetobacter baylyi ADP1

50.097

92.157

0.462
  pilF Neisseria gonorrhoeae MS11

44.504

100

0.447
  pilB/pilB1 Synechocystis sp. PCC 6803

37.374

100

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.508

100

0.369
  pilF Thermus thermophilus HB27

36.121

100

0.362

Multiple sequence alignment