Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LJX93_RS25185 Genome accession   NZ_CP085704
Coordinates   5565425..5566693 (-) Length   422 a.a.
NCBI ID   WP_227696514.1    Uniprot ID   -
Organism   Pseudomonas sp. HN2-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5560425..5571693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJX93_RS25170 (LJX93_25170) - 5562067..5563692 (-) 1626 WP_033702003.1 SPOR domain-containing protein -
  LJX93_RS25175 (LJX93_25175) aroB 5563708..5564805 (-) 1098 WP_047582202.1 3-dehydroquinate synthase -
  LJX93_RS25180 (LJX93_25180) aroK 5564902..5565420 (-) 519 WP_033702005.1 shikimate kinase AroK -
  LJX93_RS25185 (LJX93_25185) pilQ 5565425..5566693 (-) 1269 WP_227696514.1 type IV pilus secretin PilQ Machinery gene
  LJX93_RS25190 (LJX93_25190) - 5566617..5567615 (-) 999 WP_227696515.1 pilus assembly protein PilP -
  LJX93_RS25195 (LJX93_25195) - 5567630..5568166 (-) 537 WP_033702008.1 PilN domain-containing protein -
  LJX93_RS25200 (LJX93_25200) pilM 5568163..5569026 (-) 864 WP_227696516.1 type IV pilus biogenesis protein PilM -
  LJX93_RS25205 (LJX93_25205) - 5569208..5571658 (+) 2451 WP_371856397.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46054.97 Da        Isoelectric Point: 5.0904

>NTDB_id=619379 LJX93_RS25185 WP_227696514.1 5565425..5566693(-) (pilQ) [Pseudomonas sp. HN2-3]
MKKLWKWMVAWVLAIPLASAMPYRGEPLSLNFQDVQVRAVLQVLADYAGVNLVASDAVQGNITLRLQDVPWDQALDLVLR
SKGLARREEGNVLLVAPAAELAGQSLDARMGQVLDAQLQPLQRELMPIHHAEASALAQLLLATLADDGILTGRGGLSVDA
RTNTLVAYQPADRLDELRQLVAQLDVPVRQVAIEARIVEASVDYEKSLGVRWGGPLYDERVRPGKELFVDLGVERAGSSV
GLGVLRGGILLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVSFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDVLQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=619379 LJX93_RS25185 WP_227696514.1 5565425..5566693(-) (pilQ) [Pseudomonas sp. HN2-3]
ATGAAGAAGCTCTGGAAATGGATGGTGGCGTGGGTCTTGGCGATCCCGCTGGCGTCGGCAATGCCCTATCGGGGTGAGCC
ACTGTCGCTGAACTTTCAGGATGTGCAGGTGCGCGCGGTGTTGCAGGTGCTGGCGGACTACGCCGGTGTGAACCTGGTCG
CCAGCGACGCGGTGCAGGGCAATATCACCCTGCGGCTTCAGGATGTGCCCTGGGACCAGGCGCTTGATCTGGTGCTGCGC
AGCAAGGGCCTGGCCCGTCGCGAAGAGGGTAATGTGCTGCTGGTGGCGCCAGCGGCCGAGCTTGCCGGGCAGTCGCTCGA
CGCCCGCATGGGCCAGGTGCTGGATGCCCAGTTGCAACCCCTGCAGCGTGAGCTGATGCCGATCCATCACGCCGAAGCTT
CAGCGCTCGCGCAACTGCTGCTGGCGACACTGGCGGACGACGGCATCCTGACCGGGCGAGGCGGTTTGAGCGTCGATGCG
CGCACCAACACCCTGGTGGCCTACCAACCCGCCGATCGCCTGGACGAGCTACGCCAACTGGTGGCGCAGCTGGATGTGCC
GGTGCGCCAGGTGGCGATCGAGGCGCGTATTGTCGAGGCCAGCGTCGATTACGAAAAGAGCCTAGGGGTGCGTTGGGGTG
GGCCTTTGTACGATGAGCGCGTGCGGCCAGGCAAGGAGCTGTTCGTCGACCTTGGCGTGGAGCGTGCCGGCTCCAGTGTG
GGCCTGGGTGTGCTGCGCGGGGGCATCTTGCTCGACCTTGAACTCAGTGCCATGGAGAAGAGCGGCAATGGCGAAATCAT
CTCGCAGCCGAAGGTGGTCACGGCCGACAAGGAAACCGCCAGGATCCTCAAGGGCACTGAAGTGCCTTACCAGGAAACCA
GCAAGAGCGGCGCCACTTCGGTGTCGTTTCGCGAGGCTTCGCTGTCGCTCGAAGTCACCCCGCAAATCACCCCGGACAAC
AAGGTCATCATGACCGTCAGGGTGACCAAGGACGAGCCTGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAA
GAACGAGGTCAATGCCAAGGTTCGCGTGGCCGACGGCGAGACGATCGTGATCGGTGGCGTCTACTCGACCACGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGTATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.404

100

0.614
  pilQ Acinetobacter baumannii D1279779

42.266

100

0.46
  pilQ Acinetobacter baumannii strain A118

42.266

100

0.46
  comQ Acinetobacter baylyi ADP1

42.667

100

0.455
  pilQ Legionella pneumophila strain ERS1305867

42.823

99.052

0.424
  pilQ Legionella pneumophila strain Lp02

42.823

99.052

0.424
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.043

99.763

0.419
  pilQ Vibrio cholerae strain A1552

42.043

99.763

0.419
  pilQ Vibrio campbellii strain DS40M4

39.815

100

0.408