Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   VAPA_RS21640 Genome accession   NC_022247
Coordinates   4636634..4637863 (+) Length   409 a.a.
NCBI ID   WP_021008898.1    Uniprot ID   T1XGI2
Organism   Variovorax paradoxus B4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4631634..4642863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VAPA_RS21610 (VAPA_1c43730) cgtA 4631710..4632798 (-) 1089 WP_021008895.1 Obg family GTPase CgtA -
  VAPA_RS21615 (VAPA_1c43740) rpmA 4632869..4633126 (-) 258 WP_015866991.1 50S ribosomal protein L27 -
  VAPA_RS21620 (VAPA_1c43750) rplU 4633139..4633450 (-) 312 WP_015866992.1 50S ribosomal protein L21 -
  VAPA_RS21625 (VAPA_1c43760) - 4633639..4634631 (+) 993 WP_021008896.1 polyprenyl synthetase family protein -
  VAPA_RS21635 (VAPA_1c43780) pilB 4634871..4636604 (+) 1734 WP_021008897.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VAPA_RS21640 (VAPA_1c43790) pilC 4636634..4637863 (+) 1230 WP_021008898.1 type II secretion system F family protein Machinery gene
  VAPA_RS21645 (VAPA_1c43800) - 4637863..4638828 (+) 966 WP_021008899.1 prepilin peptidase -
  VAPA_RS21650 (VAPA_1c43810) coaE 4638839..4639444 (+) 606 WP_021008900.1 dephospho-CoA kinase -
  VAPA_RS21655 (VAPA_1c43820) - 4639523..4641016 (+) 1494 WP_021008901.1 MBOAT family O-acyltransferase -
  VAPA_RS21660 (VAPA_1c43830) - 4641023..4642042 (+) 1020 WP_021008902.1 hypothetical protein -
  VAPA_RS21665 (VAPA_1c43840) zapD 4642079..4642834 (+) 756 WP_021008903.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44734.36 Da        Isoelectric Point: 9.6463

>NTDB_id=61934 VAPA_RS21640 WP_021008898.1 4636634..4637863(+) (pilC) [Variovorax paradoxus B4]
MATVASTRTSNTLKEFVYEWEGKDRNGKLVRGELRAAGENQVQAALRRQGVLASKIKKRRMRSGKSIKPKDIAIFTRQLA
TMMKAGVPLLQSFDIVGRGNANPSVAKLLNDIRSDVETGTSLSAAFRKFPKYFDNLYCNLVEAGEAAGILEDLLDRLATY
MEKTEAIKSKIKSALMYPTSVVVVAFVVVAIIMIFVIPAFKQVFTSFGADLPAPTLFVMAMSEFFVSYWWLIFGVIGGGT
YFFLQAWKRNERVQRVMDRALLRVPIFGTLIEKSCVARWTRTLATMFAAGVPLVEALDSVGGASGNTVYGDATAKIQQEV
STGTSLTTAMTNVNLFPSMVIQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGVIIGGIVVSMYL
PIFKLGQVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=61934 VAPA_RS21640 WP_021008898.1 4636634..4637863(+) (pilC) [Variovorax paradoxus B4]
ATGGCAACAGTGGCATCCACCCGCACCTCGAACACGCTCAAGGAATTTGTCTACGAGTGGGAAGGCAAGGACCGCAACGG
GAAGCTGGTGCGCGGCGAGCTTCGGGCCGCCGGCGAGAACCAGGTGCAGGCCGCCCTGCGGCGCCAGGGCGTTCTGGCGT
CCAAGATCAAGAAGCGCCGCATGCGCTCGGGCAAGTCCATCAAGCCCAAGGACATTGCGATCTTCACGCGCCAGCTGGCA
ACAATGATGAAGGCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCGTGGGCCGCGGCAATGCCAACCCGAGCGTGGCCAA
GCTGCTCAACGACATCCGCAGCGACGTGGAGACCGGCACCTCGCTGTCGGCGGCCTTCCGCAAGTTTCCGAAGTATTTCG
ACAACCTCTACTGCAACCTGGTGGAGGCCGGCGAAGCGGCCGGTATCCTGGAAGACCTGCTGGACCGCCTGGCCACCTAC
ATGGAGAAGACCGAGGCCATCAAGTCGAAGATCAAGTCGGCGCTGATGTATCCCACCTCCGTGGTGGTGGTCGCGTTCGT
GGTGGTGGCCATCATCATGATCTTCGTGATTCCGGCCTTCAAGCAGGTGTTCACCTCCTTCGGCGCCGACCTGCCCGCAC
CCACGCTGTTCGTGATGGCCATGAGCGAATTCTTCGTCTCCTACTGGTGGCTGATCTTCGGCGTGATCGGCGGCGGCACC
TACTTTTTCCTGCAGGCCTGGAAGCGCAACGAACGCGTGCAGCGGGTCATGGACCGCGCGCTGCTGCGCGTGCCGATCTT
CGGCACGCTGATCGAGAAATCGTGCGTGGCCCGCTGGACCCGCACCCTTGCCACCATGTTTGCCGCCGGCGTTCCGCTGG
TCGAGGCACTCGACTCGGTGGGCGGCGCTTCGGGCAACACCGTCTACGGCGACGCCACGGCCAAGATCCAGCAGGAGGTC
TCGACCGGCACGAGCCTCACGACGGCCATGACCAACGTCAACCTGTTCCCCTCGATGGTGATCCAGATGACGGCCATCGG
CGAAGAGTCCGGCTCCATCGACCACATGCTCGGCAAGGCCGCCGACTTCTACGAGTCCGAGGTGGACGACATGGTGGCCG
GCCTCTCGAGCCTGATGGAGCCCATCATCATCGTGTTCCTGGGCGTGATCATCGGCGGCATCGTGGTGTCGATGTACCTG
CCCATCTTCAAGTTGGGCCAGGTCGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB T1XGI2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.75

97.8

0.526
  pilG Neisseria gonorrhoeae MS11

52.525

96.822

0.509
  pilG Neisseria meningitidis 44/76-A

52.273

96.822

0.506
  pilC Acinetobacter baylyi ADP1

51.759

97.311

0.504
  pilC Acinetobacter baumannii D1279779

49.622

97.066

0.482
  pilC Legionella pneumophila strain ERS1305867

48.883

98.533

0.482
  pilC Vibrio cholerae strain A1552

40.441

99.756

0.403
  pilC Thermus thermophilus HB27

38.663

100

0.396
  pilC Vibrio campbellii strain DS40M4

39.651

98.044

0.389

Multiple sequence alignment