Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LCH97_RS09690 Genome accession   NZ_CP085530
Coordinates   2039697..2041409 (-) Length   570 a.a.
NCBI ID   WP_370630685.1    Uniprot ID   -
Organism   Vogesella sp. XCS3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2034697..2046409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH97_RS09660 (LCH97_09660) - 2034710..2035948 (+) 1239 WP_227301542.1 N-acetylmuramoyl-L-alanine amidase -
  LCH97_RS09665 (LCH97_09665) yacG 2036024..2036209 (-) 186 WP_081600596.1 DNA gyrase inhibitor YacG -
  LCH97_RS09670 (LCH97_09670) zapD 2036212..2036997 (-) 786 WP_227301543.1 cell division protein ZapD -
  LCH97_RS09675 (LCH97_09675) coaE 2036994..2037611 (-) 618 WP_227301544.1 dephospho-CoA kinase -
  LCH97_RS09680 (LCH97_09680) pilD 2037604..2038458 (-) 855 WP_227301545.1 A24 family peptidase Machinery gene
  LCH97_RS09685 (LCH97_09685) pilC 2038458..2039678 (-) 1221 WP_227301546.1 type II secretion system F family protein Machinery gene
  LCH97_RS09690 (LCH97_09690) pilF 2039697..2041409 (-) 1713 WP_370630685.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LCH97_RS09695 (LCH97_09695) - 2041560..2042537 (-) 978 WP_227301547.1 porin -
  LCH97_RS09700 (LCH97_09700) - 2042843..2044249 (+) 1407 WP_227301548.1 deoxyribodipyrimidine photo-lyase -
  LCH97_RS09705 (LCH97_09705) - 2044353..2044739 (-) 387 WP_227301549.1 ComEA family DNA-binding protein -
  LCH97_RS09710 (LCH97_09710) - 2044807..2045514 (-) 708 WP_227301550.1 inner membrane protein YpjD -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 61900.28 Da        Isoelectric Point: 5.4314

>NTDB_id=618859 LCH97_RS09690 WP_370630685.1 2039697..2041409(-) (pilF) [Vogesella sp. XCS3]
MSMDAASISGLGRALVQSEHLLVQDAESIQRQAQASGISFVEQLVLSKKLTPLAIAKFACSMFGLPLLDLSTVSSDSLPK
GVVDDELVAGKRLLPLYKRGTRLFVGTSDPTQTAAFHAITFKTGLTVDVVVVEDDKLGKLVGRYSENAAVAAFNSMAADE
FGELEIADPDTEAVPDGMPDVDDAPVVKFVNKILLDAINGGASDIHFEPYERFYRVRYRIDGNLKEIVQPPLSIKEKIAS
RIKVVSRMDISEKRVPQDGRLKLKISSTKAVDFRVSTLPTLYGEKIVMRILDASAASLDIEQLGFEPSQKALLLRAIERP
YGMVLVTGPTGSGKTVSLYTCLNILNKPDTNISTAEDPVEINLPGINQVNVNERAGLTFASALRSFLRQDPDVIMVGEIR
DFETADISIKAAQTGHMVFSTLHTNNAPATLTRLLNMGVAPFNIASSVLLIMAQRLARKLCNHCKKPVDIPHEALLRAGF
AEEELDGSWQPYGPVGCDECRGSGYKGRTGIYEVMPITDAMVRLIMSDGNAIDIADLAKKEGMVDLRHSGLLKVKRGVTS
LTEIEAVTNE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=618859 LCH97_RS09690 WP_370630685.1 2039697..2041409(-) (pilF) [Vogesella sp. XCS3]
ATGTCTATGGACGCTGCTTCGATTTCCGGTCTCGGTCGTGCTTTGGTGCAAAGCGAACATCTGTTGGTGCAAGACGCAGA
AAGCATCCAGCGTCAGGCCCAGGCATCTGGCATTTCATTCGTAGAGCAATTGGTGCTGAGCAAAAAGCTTACGCCGCTGG
CGATTGCCAAGTTTGCCTGCAGCATGTTTGGTTTACCTTTGCTGGACTTGTCTACGGTCAGTAGTGACAGCCTCCCCAAA
GGCGTGGTGGACGACGAATTGGTGGCGGGTAAGCGCTTATTGCCATTGTATAAACGTGGTACGCGCTTGTTTGTCGGGAC
GTCTGATCCGACACAAACTGCGGCGTTTCACGCCATTACCTTCAAGACTGGTCTGACTGTAGATGTGGTGGTGGTCGAGG
ACGACAAGCTAGGCAAGCTGGTTGGCCGCTACAGCGAGAATGCCGCCGTTGCCGCATTTAACAGTATGGCGGCGGACGAG
TTTGGCGAGCTGGAAATCGCCGACCCGGATACCGAGGCGGTACCGGATGGCATGCCGGATGTAGATGATGCGCCGGTGGT
GAAGTTTGTAAACAAGATCCTGCTGGATGCGATTAATGGTGGTGCCTCGGATATCCATTTCGAACCGTACGAGCGCTTTT
ACCGCGTGCGCTACCGTATCGATGGCAATCTGAAAGAGATTGTGCAGCCACCATTATCGATCAAAGAAAAGATTGCCTCG
CGAATCAAGGTGGTCTCGCGCATGGATATCTCGGAAAAGCGTGTGCCGCAGGATGGCCGCCTGAAACTGAAAATATCCAG
TACCAAGGCTGTGGACTTCCGGGTCAGCACGCTACCTACGCTATATGGCGAGAAAATCGTGATGCGTATCCTGGATGCGT
CTGCCGCCTCGCTGGATATCGAACAACTAGGGTTTGAACCCAGCCAGAAGGCACTGTTACTACGAGCCATTGAGCGGCCG
TATGGCATGGTGCTGGTAACCGGGCCCACCGGTAGCGGTAAAACGGTTTCCTTGTACACCTGCCTGAATATCCTGAATAA
ACCGGATACCAATATTTCTACCGCGGAAGACCCGGTGGAAATCAACTTGCCGGGCATCAACCAGGTTAACGTCAACGAGC
GTGCGGGCCTGACGTTTGCCTCGGCTTTGCGTTCGTTTCTGCGTCAGGATCCGGACGTGATCATGGTGGGTGAGATTCGC
GACTTCGAGACCGCGGATATTTCTATCAAGGCAGCCCAGACCGGCCACATGGTGTTTTCTACGCTGCACACCAATAATGC
ACCAGCGACGCTGACCCGTTTGTTGAATATGGGCGTAGCACCGTTCAACATTGCCAGCTCGGTGCTGCTGATCATGGCGC
AGCGTCTGGCGCGCAAGCTGTGTAACCACTGCAAAAAGCCGGTGGATATCCCGCATGAGGCGCTACTGCGTGCTGGCTTT
GCCGAAGAAGAACTGGACGGTAGCTGGCAGCCTTATGGCCCGGTGGGGTGCGACGAATGCCGTGGTTCCGGCTATAAAGG
CCGTACCGGTATTTATGAGGTAATGCCTATTACTGATGCCATGGTGCGTTTGATCATGTCGGACGGTAACGCGATAGACA
TTGCCGACCTTGCCAAGAAAGAGGGCATGGTGGATCTGCGTCACTCTGGTTTGTTGAAGGTAAAGCGGGGCGTTACCTCG
TTGACCGAAATTGAAGCCGTTACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(62-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

55.83

99.298

0.554
  pilB Acinetobacter baumannii D1279779

54.561

100

0.546
  pilB Acinetobacter baylyi ADP1

54.255

98.947

0.537
  pilB Legionella pneumophila strain ERS1305867

52.705

100

0.53
  pilB Vibrio parahaemolyticus RIMD 2210633

48.673

99.123

0.482
  pilB Vibrio campbellii strain DS40M4

48.319

99.123

0.479
  pilB Vibrio cholerae strain A1552

48.925

97.895

0.479
  pilB/pilB1 Synechocystis sp. PCC 6803

36.731

100

0.398
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.89

95.439

0.381
  pilF Thermus thermophilus HB27

39.344

96.316

0.379