Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LF296_RS16775 Genome accession   NZ_CP085083
Coordinates   3570866..3572578 (+) Length   570 a.a.
NCBI ID   WP_004774090.1    Uniprot ID   N9Q1F4
Organism   Acinetobacter vivianii strain KJ-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3565866..3577578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LF296_RS16740 (LF296_16740) nusA 3566687..3568171 (-) 1485 WP_004774096.1 transcription termination factor NusA -
  LF296_RS16745 (LF296_16745) rimP 3568209..3568733 (-) 525 WP_004774094.1 ribosome maturation factor RimP -
  LF296_RS16765 (LF296_16765) secG 3569361..3569690 (-) 330 WP_005255466.1 preprotein translocase subunit SecG -
  LF296_RS16770 (LF296_16770) tpiA 3569703..3570494 (-) 792 WP_272654995.1 triose-phosphate isomerase -
  LF296_RS16775 (LF296_16775) pilB 3570866..3572578 (+) 1713 WP_004774090.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LF296_RS16780 (LF296_16780) pilC 3572603..3573829 (+) 1227 WP_004774089.1 type II secretion system F family protein Machinery gene
  LF296_RS16785 (LF296_16785) pilD 3573835..3574695 (+) 861 WP_047431455.1 prepilin peptidase Machinery gene
  LF296_RS16790 (LF296_16790) coaE 3574697..3575302 (+) 606 WP_047431458.1 dephospho-CoA kinase -
  LF296_RS16795 (LF296_16795) - 3575299..3576210 (-) 912 WP_272654996.1 DMT family transporter -
  LF296_RS16800 (LF296_16800) rlmB 3576242..3576988 (-) 747 WP_004774084.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LF296_RS16805 (LF296_16805) - 3577182..3577508 (-) 327 WP_004774082.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63175.43 Da        Isoelectric Point: 5.1411

>NTDB_id=615128 LF296_RS16775 WP_004774090.1 3570866..3572578(+) (pilB) [Acinetobacter vivianii strain KJ-1]
MSGLHTSPKFSGFIRRLVEDSHLSAATMQSAIDAAKRAQQDIVAHLIEHHRLSPLTIAETIAAEFGEPLFDLAVYDTALL
PRDLADNKLIQKHRVAPLLQRGQILYVATSNPTNIEALDAIRFNTKLNIEPIIVEHTKLEKLIEQQFGDSGSFDFGDSED
FDLDIDVSQDPAKEEDDAPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQMATRLAS
RLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKTPIDIPKQSLLELGF
TEQDMADPDFKIFQPVGCSECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASEQAGFSNLRRSGLRKVMQGITS
LQEINRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=615128 LF296_RS16775 WP_004774090.1 3570866..3572578(+) (pilB) [Acinetobacter vivianii strain KJ-1]
ATGTCAGGATTACATACGTCTCCAAAGTTTTCGGGGTTTATTCGACGGTTAGTTGAAGACAGTCATCTGTCTGCGGCAAC
CATGCAATCAGCGATTGATGCTGCTAAAAGAGCACAACAAGACATCGTCGCACACTTAATTGAACATCATCGTTTATCGC
CGCTTACCATTGCTGAAACCATTGCAGCCGAGTTTGGCGAGCCTCTATTTGATCTGGCAGTCTACGATACTGCCTTGCTG
CCGCGTGATCTGGCTGACAACAAACTGATTCAGAAACATAGAGTCGCTCCCCTGCTGCAACGAGGCCAGATTCTATACGT
TGCCACCAGTAACCCGACCAATATCGAAGCTCTGGATGCAATTCGCTTTAATACCAAGCTCAATATCGAACCGATCATTG
TTGAACACACTAAATTAGAAAAACTGATTGAACAGCAATTTGGGGATTCGGGGAGTTTTGACTTTGGTGATAGCGAAGAT
TTTGATCTGGATATTGATGTAAGTCAGGATCCAGCAAAAGAAGAAGACGATGCCCCACAAGGCGATGAGTCACCAATTGT
TAAATATATCAATAAATTACTGATTGATGCGATTCGCATGGGTGCATCCGATTTACATTTTGAACCTTATGAAAAAATGT
ATCGGGTTCGCTATCGCGTTGATGGTGTACTCCGTCAAATTGCCAATCCTCCTTTACAAATGGCAACCCGTTTAGCGTCT
CGTTTAAAAGTTATGTCGCAAATGGACATTTCAGAAAAACGGATGCCTCAAGATGGTCGTATTAAATTAAAGCTCTCTAA
AACCAAAGCCATCGACTTCCGTGTGAACTCACTGCCTACGCTATTTGGTGAGAAGCTGGTGCTGCGTATCCTTGATCCAT
CCAGCGCCATGCTCGGAATCGAAGCACTCGGTTACGAAGATGAGCAAAAAGCTTTATTCATGGAAGCGCTGGATAAGCCT
CAAGGTATGCTTCTGATCACGGGCCCAACGGGTTCTGGTAAAACGGTCTCGTTATATACAGGCCTCAATATTTTAAATAC
TGAAGATACCAATATTTCCACGGCCGAAGATCCTGTCGAAATCAATCTGGAAGGGATTAATCAGGTCAACGTCAATCCTA
AAACTGGCCTCACCTTCTCTGCTGCATTGAAAGCCTTCTTACGCCAGGATCCAGACATCATCATGGTGGGTGAGATTCGA
GATCTGGAAACAGCAGAAATTGCCATTAAAGCTGCCCAAACAGGCCATATGGTAATGTCGACACTGCATACCAACAGTGC
ACCTGAAACCTTGACCCGTTTACGTAATATGGGCGTACCGTCCTTCAATATTGCCACTTCAGTGAATCTGGTGATTGCAC
AACGCTTGGCACGTCGCTTATGCTCTCAGTGTAAGACACCGATCGACATACCCAAACAAAGCCTTTTAGAGCTTGGCTTT
ACCGAACAGGATATGGCTGATCCTGATTTCAAAATTTTTCAGCCTGTGGGTTGTTCTGAATGTCGTGAAGGCTACAAAGG
CCGCGTCGGTGTTTATGAAGTCATGAAAGTCACGCCAGAAATTTCTAGAATTATTATGGAAGACGGCAATGCCTTACAAA
TTGCAGCCGCATCAGAACAAGCAGGTTTTAGTAATTTACGTCGTTCAGGTTTAAGAAAGGTGATGCAAGGAATTACCTCT
TTGCAGGAAATTAACCGCGTGACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9Q1F4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

84.063

100

0.842
  pilB Acinetobacter baylyi ADP1

76.789

100

0.772
  pilB Legionella pneumophila strain ERS1305867

53.918

94.035

0.507
  pilF Neisseria gonorrhoeae MS11

49.378

98.772

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

51.931

90.877

0.472
  pilB Vibrio cholerae strain A1552

48.551

96.842

0.47
  pilB Vibrio campbellii strain DS40M4

50.579

90.877

0.46
  pilF Thermus thermophilus HB27

37.857

98.246

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.171

93.158

0.365
  pilB/pilB1 Synechocystis sp. PCC 6803

33.713

100

0.363